LOCUS       Exported               12258 bp ds-DNA    circular SYN 10-DEC-2018
DEFINITION  Clone#4 sequence correct
            WTC11 iPSCs red after nucleofection.
ACCESSION   .
VERSION     .
KEYWORDS    pRT029_F8 CLYBL-CAG-DHFR-dCas9-BFP-KRAB-NLS-DHFR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2019 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..12258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4158..10888
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4164..10352
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4164..4637
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          9828..10352
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(395..1255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1273..2110
                     /note="PCR #A1 product"
     misc_feature    1273..2104
                     /label="C13-LA"
                     /note="C13-LA"
     misc_feature    2090..2445
                     /note="PCR product"
     misc_feature    2113..2161
                     /note="added back in primer"
     misc_feature    2113..2161
                     /note="PCR #A2"
     misc_feature    2113..2138
                     /note="Splice acceptor"
     misc_feature    2162..2164
                     /note="post-splice stop codon for CLYBL gene product"
     misc_signal     2165..2248
                     /note="SeqVer (AAV F)"
     misc_signal     2165..2185
                     /note="SeqVer (AAV F)"
     polyA_signal    2186..2410
                     /note="bGHpolyA"
                     /note="bovine growth hormone polyadenylation signal
                     Common feature added/modified by Mo Mandegar"
     enhancer        2416..2795
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2797..4044
                     /label="CAG"
                     /note="CAG"
                     /note="Common feature added by Mo Mandegar"
     intron          4052..4145
                     /label="Mini-Intron"
                     /note="Mini-Intron"
                     /note="Common feature added by Mo Mandegar"
     misc_feature    4146..4151
                     /note="SalI site/Kozak sequence"
     misc_feature    4152..4157
                     /note="NheI site added for cloning"
     misc_signal     complement(4164..4640)
                     /note="SeqVer (KRAB R)"
     misc_signal     4164..4640
                     /note="SeqVer (TRE F)"
                     /note="Common feature added by Mo Mandegar"
     misc_signal     4164..4640
                     /note="SeqVer 3/14/13"
     CDS             4164..4637
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /note="ecDHFR (R12Y, Y100I)"
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILER"
     misc_feature    4638..4640
                     /note="GSGSG Linker"
     misc_feature    4650..4661
                     /note="GSGGTSAT"
     misc_feature    4651..4655
                     /note="part of primer"
     misc_feature    4656..9827
                     /note="PCR product"
     misc_feature    4656..4676
                     /note="Feature 27"
     CDS             4662..9827
                     /codon_start=1
                     /label="dCas9-HA-NLS-BFP-KRAB"
                     /note="dCas9-HA-NLS-BFP-KRAB"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDAYPYDVPDYASLGSGSPKKKRKVEDPKKKRKVDG
                     IGSGSNGSSGSSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGG
                     PLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDT
                     SLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGG
                     SHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPS
                     KLGHKLNGGGGGMDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENY
                     KNLVSLGYQLTKPDVILRLEKGEEP"
     misc_feature    4662..8765
                     /note="dCas9"
     misc_feature    complement(8185..8934)
                     /note="QB4721"
     misc_feature    8769..8795
                     /label="HA"
                     /note="HA"
     misc_feature    8814..8834
                     /label="SV40 NLS"
                     /note="SV40 NLS"
     misc_feature    8841..8861
                     /note="SV40 NLS"
     misc_feature    8901..9596
                     /note="BFP"
     misc_feature    9597..9827
                     /note="KRAB"
     CDS             9828..9875
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9876..10352
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /note="ecDHFR"
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     misc_feature    10362..10888
                     /label="Rabbit beta-globin polyA signal"
                     /note="Rabbit beta-globin polyA signal"
     misc_signal     complement(10889..10891)
                     /note="SeqVer (SV40 F)"
     misc_signal     complement(10889..10891)
                     /note="SeqVer (WT AAV R)"
     misc_feature    10895..11690
                     /label="C13T1-RA"
                     /note="C13T1-RA"
     misc_recomb     11691..11846
                     /label="AAV 3' Arm"
                     /note="AAV 3' Arm"
                     /note="Common feature added by Mo Mandegar"
     rep_origin      complement(11894..224)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
       61 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      301 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      721 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      781 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      841 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      901 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      961 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1021 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1081 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1141 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1201 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1261 tcctttttca atagcaggag aatcatgcca atgggccaat atacattctg acccacagtt
     1321 tcataataaa ataaaatggt tgtggttgta agccactatg tttcagagtg gtttgttaca
     1381 cagcaataaa taactaatat agtaggcata ccatcaagtc caaagtaggt agagaagaat
     1441 gtaaatagca gagcaaaaca gcatgactgg tggctgggag gcttaaaact gggacaggat
     1501 cagagtcatg aaagaagtca aagaaatggt tcagaagtaa ggctgagact gacttacaaa
     1561 agctgaaagt ccctttaagt tggtgtttgg tgcattggca ggggcaggta tggtgactta
     1621 aaagagccat gctcaacaag atcaagcaca acacaatcac gggtcacccc agcagacctt
     1681 agcgagtcta gccatttctt tggtggtggt cacagtcatg cttcagccca gtttccactt
     1741 ggacaaatgg tacatatttt caatgagatg aaaattaaga tacaatccat gtgctcagag
     1801 agtgatcaca gctctgacta aacactgtgc cccaaagtgt tgaggaattg ggaaaaccta
     1861 gctgagttag tggtctcttt tctgttacaa taaagctcat aatgaaaatt agccttcttt
     1921 gttcttcccc aagtctttct ttctagacga aactactttc aactgtttta acttccttac
     1981 tgttaacttc catatttcta gatagtatgg tcagactgtt atttcttgac ttttaaatgt
     2041 aagatattat ctactgactt ccttctatgt aagatgagga ttgagctctc ttacccttct
     2101 cccagcggcc gcctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg
     2161 atagaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     2221 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2281 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2341 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2401 ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca attacggggt
     2461 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     2521 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     2581 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     2641 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     2701 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     2761 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
     2821 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
     2881 tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc
     2941 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     3001 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     3061 gcgcgcggcg ggcggggagt cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc
     3121 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     3181 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     3241 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     3301 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     3361 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     3421 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     3481 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     3541 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     3601 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     3661 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     3721 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     3781 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     3841 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     3901 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     3961 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     4021 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     4081 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     4141 agaatgtcga cgctagcgcc accatgatca gtctgattgc ggcgttagcg gtagattacg
     4201 ttatcggcat ggaaaacgcc atgccgtgga acctgcctgc cgatctcgcc tggtttaaac
     4261 gcaacacctt aaataaaccc gtgattatgg gccgccatac ctgggaatca atcggtcgtc
     4321 cgttgccagg acgcaaaaat attatcctca gcagtcaacc gagtacggac gatcgcgtaa
     4381 cgtgggtgaa gtcggtggat gaagccatcg cggcgtgtgg tgacgtacca gaaatcatgg
     4441 tgattggcgg cggtcgcgtt attgaacagt tcttgccaaa agcgcaaaaa ctgtatctga
     4501 cgcatatcga cgcagaagtg gaaggcgaca cccatttccc ggattacgag ccggatgact
     4561 gggaatcggt attcagcgaa ttccacgatg ctgatgcgca gaactctcac agctattgct
     4621 ttgagattct ggagcggggc tctggaggga ctagtgccac catggacaag aagtacagca
     4681 tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
     4741 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
     4801 tgatcggcgc cctgctgttc gacagcggag aaacagccga ggccacccgg ctgaagagaa
     4861 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
     4921 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
     4981 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
     5041 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
     5101 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
     5161 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
     5221 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
     5281 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg
     5341 cccagctgcc cggcgagaag aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg
     5401 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga
     5461 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg
     5521 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
     5581 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg
     5641 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt
     5701 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatc gatggcggag
     5761 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg
     5821 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca
     5881 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg
     5941 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc
     6001 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca
     6061 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgcca
     6121 gcgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg
     6181 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtacaacgag ctgaccaaag
     6241 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaaag
     6301 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg
     6361 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt
     6421 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc
     6481 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg
     6541 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag
     6601 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga
     6661 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg
     6721 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
     6781 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc
     6841 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc
     6901 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga
     6961 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
     7021 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
     7081 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg
     7141 aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct cagagctttc
     7201 tgaaggacga ctccatcgat aacaaagtgc tgactcggag cgacaagaac cggggcaaga
     7261 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cgccagctgc
     7321 tgaatgccaa gctgattacc cagaggaagt tcgacaatct gaccaaggcc gagagaggcg
     7381 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
     7441 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaacg
     7501 acaaactgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc
     7561 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg
     7621 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg
     7681 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
     7741 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca
     7801 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg
     7861 gcgaaacagg cgagatcgtg tgggataagg gccgggactt tgccaccgtg cggaaagtgc
     7921 tgtctatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
     7981 aagagtctat cctgcccaag aggaacagcg acaagctgat cgccagaaag aaggactggg
     8041 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg
     8101 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
     8161 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
     8221 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
     8281 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg
     8341 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
     8401 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaaa cactacctgg
     8461 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
     8521 tggacaaggt gctgagcgcc tacaacaagc acagagacaa gcctatcaga gagcaggccg
     8581 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact
     8641 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc
     8701 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
     8761 gcgacgccta tccctatgac gtgcccgatt atgccagcct gggcagcggc tcccccaaga
     8821 aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt ggacggcatt ggtagtggga
     8881 gcaacggcag cagcggatcc agcgagctga ttaaggagaa catgcacatg aagctgtaca
     8941 tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct
     9001 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     9061 tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg
     9121 gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     9181 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     9241 tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga
     9301 agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg
     9361 aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca
     9421 agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg
     9481 tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg
     9541 aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatggcg
     9601 gtggcggagg gatggatgct aagtcactaa ctgcctggtc ccggacactg gtgaccttca
     9661 aggatgtatt tgtggacttc accagggagg agtggaagct gctggacact gctcagcaga
     9721 tcgtgtacag aaatgtgatg ctggagaact ataagaacct ggtttccttg ggttatcagc
     9781 ttactaagcc agatgtgatc ctccggttgg agaagggaga agagcccaaa aggccggcgg
     9841 ccacgaaaaa ggccggccag gcaaaaaaga aaaagatcag tctgattgcg gcgttagcgg
     9901 tagatcacgt tatcggcatg gaaaccgtca tgccgtggaa cctgcctgcc gatctcgcct
     9961 ggtttaaacg caacacctta aataaacccg tgattatggg ccgccatacc tgggaatcaa
    10021 tcggtcgtcc gttgccagga cgcaaaaata ttatcctcag cagtcaaccg agtacggacg
    10081 atcgcgtaac gtgggtgaag tcggtggatg aagccatcgc ggcgtgtggt gacgtaccag
    10141 aaatcatggt tattggcggc ggtcgcgttt atgaacagtt cttgccaaaa gcgcaaaaac
    10201 tgtatctgac gcatatcgac gcagaagtgg aaggcgacac ccatttcccg gattacgagc
    10261 cggatgactg ggaatcggta ttcagcgaat tccacgatgc tgatgcgcag aactctcaca
    10321 gctattgctt tgagattctg gagcggcgat aaacgcgtga attcactcct caggtgcagg
    10381 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
    10441 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
    10501 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
    10561 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
    10621 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
    10681 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
    10741 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
    10801 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
    10861 tagctgtccc tcttctctta tggagatcga tatctcctca tccttccaac ataaatatat
    10921 tttgggatta tatcaacatt caatgttact taaagtgacc ttgtaaatat tttcacaact
    10981 gagccatgtt tgatttgtat acttatgttt actttactgt ttttcctgaa gttaataatt
    11041 gccttgaatt tatttatttc tttaaaaatg tttcattact caggactgta gtttacatta
    11101 cgattctttg tgttatacag ttgatgggtt tcttttcttt cttaatttct ttaaaaaata
    11161 gagatggggt cttactatat tacccaggct ggtcttgaag tcctgggctc aagtgatctt
    11221 cctgtctcag cctaccaagt agctgagact ataggtgcaa aaaagccact atacctggct
    11281 agtttacagg ttttaacaaa tgcattatgc cacgtatcca ttattacagg atcacacaag
    11341 atattttcat taccctgaaa gcatccctgt gttccaccaa ttcatcctgc ctccatgagc
    11401 cgctggcaac cactgatctc tatagttttg ccttttctaa aatgtcatat aattggaatc
    11461 atacagtctg tagcattttc agactagctt ttaaaatttg gcaatatgca tttaaggttc
    11521 ctccttaaat gtgaagggca tagccaatgt ggcttgatag ctcatttctt tttattggtg
    11581 aatatttcat tgtctggatg ttccacagtt tgtttatcca ttcacctatt caatttgctt
    11641 tttttctgtg tatctatcac taattcaata ctggactctc caacagagcc gtcccgcttc
    11701 cctaaggccc tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc
    11761 aggcttccct gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc
    11821 cctacagggg ttcctggctc tgctctaagg gcgaattcgc caggaaccgt aaaaaggccg
    11881 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11941 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    12001 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    12061 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    12121 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    12181 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    12241 cagcagccac tggtaaca
//
