LOCUS       Exported                7523 bp ds-DNA     circular SYN 29-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 29, 2019 from SnapGene 4.3.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      103
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
     misc_feature    complement(865..1725)
                     /label=Beta-lactamase gene (Ampr)
     rep_origin      1857..2312
                     /direction=RIGHT
                     /label=f1 origin
     misc_feature    2313..2328
                     /label=seq primer F
                     /note="seq primer F"
     misc_feature    2421..2436
                     /label=Untitled
     misc_feature    2437
                     /label=missing
                     /note="missing"
     misc_feature    2438..3046
                     /label=homology arm left
                     /note="homology arm left"
     misc_feature    3033..3046
                     /label=missing based on seq
                     /note="missing based on seq"
     polyA_signal    3052..3205
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    3154..3173
                     /label=seq primer R
                     /note="seq primer R"
     misc_feature    3293..3326
                     /label=upstream loxP site
     misc_binding    3343..3558
                     /label=ZFHD1 x12 DNA Binding Array
     misc_binding    3349..3360
                     /label=First ZFHD1 cassette
     misc_feature    3545..3560
                     /label=OH
                     /note="OH"
     misc_feature    3566..3920
                     /label=from TOPO
                     /note="from TOPO"
     misc_feature    3566
                     /label=incl
                     /note="incl"
     misc_feature    3567..3919
                     /label=from pUASp vector
                     /note="from pUASp vector"
     misc_feature    3567..3907
                     /label=contains 14 UAS Gal4 binding sites
                     /note="contains 14 UAS Gal4 binding sites"
     primer_bind     3567..3583
                     /label=CAAGCTTAGGCCTCCAA
     misc_feature    3584..3603
                     /label=Untitled
     misc_feature    3607..3626
                     /label=Untitled
     misc_feature    3630..3649
                     /label=Untitled
     misc_feature    3653..3672
                     /label=Untitled
     misc_feature    3676..3695
                     /label=Untitled
     misc_feature    3699..3718
                     /label=Untitled
     misc_feature    3722..3741
                     /label=Untitled
     misc_feature    3745..3764
                     /label=Untitled
     misc_feature    3768..3787
                     /label=Untitled
     misc_feature    3791..3810
                     /label=Untitled
     misc_feature    3814..3833
                     /label=Untitled
     misc_feature    3837..3856
                     /label=Untitled
     misc_feature    3860..3879
                     /label=Untitled
     misc_feature    3883..3902
                     /label=Untitled
     primer_bind     complement(3903..3918)
                     /label=ATCGACTCGACCCTTG
     misc_feature    3904..3919
                     /label=OH
                     /note="OH"
     misc_feature    3920
                     /label=incl
                     /note="incl"
     misc_feature    3926..4567
                     /label=tj enhancer
                     /note="tj enhancer (D. yakuba)"
     primer_bind     3926..3951
                     /label=TCTTAATGAAGTACTTTAACTGATTG
     primer_bind     complement(4554..4567)
                     /label=ATAGCGTCGCGGGC
     misc_feature    4568..4583
                     /label=OH
                     /note="OH"
     misc_feature    4577..4731
                     /label=DSCP Drosophila synthetic core promoter
                     /note="DSCP Drosophila synthetic core promoter"
     misc_feature    4592..5754
                     /label=gblock_2_promoter_GFP
                     /note="gblock_2_promoter_GFP"
     misc_feature    4732..4764
                     /label=5UTR
                     /note="5UTR"
     misc_feature    4768..4783
                     /label=OH
                     /note="OH"
     misc_feature    4770..5266
                     /label=intron
                     /note="intron"
     misc_feature    4784..4998
                     /label=NheI
                     /note="NheI"
     primer_bind     4784..4800
                     /label=CAAACTGGGGATTCCTG
     misc_feature    4800..4818
                     /label=BoxB
                     /note="BoxB"
     misc_feature    4842..4860
                     /label=BoxB
                     /note="BoxB"
     misc_feature    4866..4884
                     /label=BoxB
                     /note="BoxB"
     misc_feature    4924..4942
                     /label=BoxB
                     /note="BoxB"
     misc_feature    4948..4966
                     /label=BoxB
                     /note="BoxB"
     primer_bind     complement(4982..4998)
                     /label=GAATGGGTGACCTCGAG
     misc_feature    4999..5014
                     /label=OH
                     /note="OH"
     primer_bind     5022..5038
                     /label=CAAACTGGGGATTCCTG
     misc_feature    5038..5056
                     /label=BoxB
                     /note="BoxB"
     misc_feature    5080..5098
                     /label=BoxB
                     /note="BoxB"
     misc_feature    5104..5122
                     /label=BoxB
                     /note="BoxB"
     misc_feature    5162..5180
                     /label=BoxB
                     /note="BoxB"
     misc_feature    5186..5204
                     /label=BoxB
                     /note="BoxB"
     misc_feature    5270..5272
                     /label=translation start
                     /note="translation start"
     CDS             5273..5986
                     /codon_start=1
                     /label=d2eGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(5315..5336)
                     /codon_start=1
                     /label=EGFP_N_primer
                     /translation="RRRPARP"
     CDS             5453..5482
                     /codon_start=1
                     /label=Y66 (EGFP)
                     /translation="VTTLTYGVQC"
     CDS             5923..5944
                     /codon_start=1
                     /label=EGFP_C_primer
                     /translation="HGPAGVR"
     misc_feature    6005..6061
                     /label=P2A
                     /note="P2A"
     CDS             6062..6484
                     /label=BlaS
     primer_bind     complement(6461..6512)
                     /label=CTGCTCGAAGGGACGCGTGAGAATTCATTTAGTTTCTGGTGTATTTCAGT
     primer_bind     6486..6522
                     /label=TGAATTCTCACGCGTCCCTTCGAGCAGACATGATAAG
     primer_bind     6503..6522
                     /label=CCGCTTCGAGCAGACATGATAAG
     polyA_signal    6510..6731
                     /label=SV40 late poly (A) signal
     misc_feature    complement(6522..6547)
                     /label=enh_seq_r
                     /note="enh_seq_r"
     misc_feature    6534..6555
                     /label=seq primer F
                     /note="seq primer F"
     primer_bind     complement(6736..6758)
                     /label=CATCGGTCGACGGATCCTTATCG
     primer_bind     complement(6761..6782)
                     /label=GACTGGGTTGAAGGCTCTCAAG
     misc_feature    6814..7383
                     /label=homology arm right
                     /note="homology arm right"
     misc_feature    7320..7383
                     /label=missign based on seq
                     /note="missign based on seq"
     misc_feature    7437..7452
                     /label=Untitled
     misc_feature    7453..7468
                     /label=Untitled
     misc_feature    complement(7503..7523)
                     /label=seq primer R
                     /note="seq primer R"
     primer_bind     complement(7503..7523)
                     /label=GCCTTTGAGTGAGCTGATACC
ORIGIN
        1 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
       61 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      541 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      841 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      901 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      961 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1021 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1081 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     1141 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     1201 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     1261 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1321 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1381 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1441 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1501 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1561 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1621 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1681 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1741 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1801 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     1861 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     1921 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     1981 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2041 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2101 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2161 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2221 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2281 gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc tgcgcaactg
     2341 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata
     2401 agtaagtaat attaaggtac gggaggtact tggagcgtaa tgtaagctag tcacgtctag
     2461 ccacttgtgt cctgagccca agctccagcc ttttccagct tttcacaggg tttccccttc
     2521 taaggacgcc ctgctctcgt ttcgagctcg tgccttcaca ttcacttagt gcaaggctgt
     2581 gccaaacctc ttttgcatat tgatatggct gctgcgaaat ccacttacct tgcacacatt
     2641 tttggtgcca aatttagacc tatttttgca agaatatata tgagttagtg tttcgactca
     2701 ttactgtccc ttcagagaga acatttcgca ttaattgtta cacacctcaa gtatttttag
     2761 aacgcgtgct caagcgccgt gtctagacga aagttttcgt caggtaacag ccgaaaacaa
     2821 attaaaacaa acgaaaacat tttggaaaac tgaccaaccc caagcacccg atgcccaaat
     2881 agatggtggt gtcctttgaa atgttgttgc aaattgttag ctcttgtccc ataaaagtgc
     2941 acataaaatc aaaaagggga aaaatgtgcg ggcctccctc ccctaacaat tcccaaacat
     3001 tttcgcatgg cacgccaagc aattaaagcg ggatctcgct gcctgtgccg caataaaata
     3061 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     3121 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     3181 agtgcaggtg ccagaacatt tctctatcga taggtacttg taatacgact cactataggg
     3241 cgaattgggt tgtaatacga ctcactatag ggcgaattgg gggctgcagg ggataacttc
     3301 gtataatgta tactatacga agttatgggg ccggccggat ccctcgagta atgatgggcg
     3361 ggaagttaat gatgggcgta aggataatga tgggcgcagg cctaatgatg ggcgctcgag
     3421 taatgatggg cggagaggta atgatgggcg gtgcagtaat gatgggcggt cgactaatga
     3481 tgggcgtgcc aataatgatg ggcgcaggct taatgatggg cgttgtggta atgatgggcg
     3541 tgcgattaat gatgggcggg atgcatcaag cttaggcctc caaggcggag tactgtcctc
     3601 cgggctggcg gagtactgtc ctccggcaag gcggagtact gtcctccggg ctggcggagt
     3661 actgtcctcc ggcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca
     3721 aggcggagta ctgtcctccg ggctggcgga gtactgtcct ccggcaaggc ggagtactgt
     3781 cctccgggct ggcggagtac tgtcctccgg caaggcggag tactgtcctc cgggctggcg
     3841 gagtactgtc ctccggcaag gcggagtact gtcctccggg ctggcggagt actgtcctcc
     3901 ggcaagggtc gagtcgattg cgcgctctta atgaagtact ttaactgatt gataatttga
     3961 acattcgcaa gtctaagaat gcagtttttc atagtactcc attatgtcat gatgtattcc
     4021 attatgtctc aggccacatt catatctggg ctttagaagc taccgagtag gtctcagctt
     4081 gcatcataga tttggggtct catctcgacg ctttccgaga agtggattct ctgtgtggtc
     4141 tgtcggcatg gataaatggg cgtccagcga tgccgaccac cgcgggcaac agtttaagaa
     4201 tcaaaataaa cacttcgatc cctccgggct ccgataactt ctgtcaacaa acttgggtcg
     4261 cgtaacggcg catgcaggag cactggagga gtatgagctc cagccgaagt gcagcattgg
     4321 attacaatcg tcaacatgga gatcagcatc agatcaatcg ccatctgccg gcccctaaaa
     4381 gaaagatact ttggagcagt cgagctttca gttggggcgg cgtaattcgg caataatcag
     4441 agggccatat ctgcatttcc aagccgcctt gcctgaaata aatcccttat aaaaatcggt
     4501 tcttttagcc tgtttgtttt cttttccatt ttgatttcac ttcttgcggt gtcgcccgcg
     4561 acgctattct gcgatcgagc tcgcccgggg atcgagcgca gcggtataaa agggcgcggg
     4621 gtggctgaga gcatcagttg tgaatgaatg ttcgagccga gcagacgtgc cgctgccttc
     4681 gttaatatcc tttgaataag ccaactttga atcacaagac gcataccaaa cggcattccg
     4741 gtactgttgg taaagccacc atggaaaagg taggttcaac cagcaaactg gggattcctg
     4801 ggccctgaag aagggcccct cgactagtcc aaatacaaac tgggccctga agaagggccc
     4861 atatagggcc ctgaagaagg gccctatcga ggatattatc tcgactaagt ccaactacaa
     4921 actgggccct gaagaagggc ccatataggg ccctgaagaa gggccctatc gaggatatta
     4981 tctcgaggtc acccattcct agcttcccta agtccaacta ccaaactggg gattcctggg
     5041 ccctgaagaa gggcccctcg actagtccaa atacaaactg ggccctgaag aagggcccat
     5101 atagggccct gaagaagggc cctatcgagg atattatctc gactaagtcc aactacaaac
     5161 tgggccctga agaagggccc atatagggcc ctgaagaagg gccctatcga ggatattatc
     5221 tcgagacacc gaaacgacta accctaattc ttatccttta cttcaggcca tggtgagcaa
     5281 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5341 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5401 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5461 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5521 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5581 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5641 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5701 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5761 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5821 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5881 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5941 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggcgg ccgcaggcag
     6001 cggcgccacc aacttcagcc tgctgaagca ggccggcgac gtggaggaga accccggccc
     6061 catgaagacc ttcaacatct ctcagcagga ccttgagctg gtggaagttg ccactgagaa
     6121 aatcaccatg ctctatgagg acaacaagca ccatgttggt gctgccatca ggaccaagac
     6181 aggagaaatc atctctgctg tccacattga agcctacatt ggcagggtca ctgtgtgtgc
     6241 agaggccatt gccattgggt ctgcagtctc caatgggcag aaggactttg acaccattgt
     6301 ggctgtgagg cacccctaca gtgatgaggt ggacaggtcc atcagagtgg tgtccccctg
     6361 tggcatgtgc agggagctca tctcagacta tgcccctgat tgctttgttc tgattgagat
     6421 gaatggcaag ctggtcaaga caaccattga ggagctgatt ccactgaaat acaccagaaa
     6481 ctaaatgaat tctcacgcgt cccttcgagc agacatgata agatacattg atgagtttgg
     6541 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     6601 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     6661 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     6721 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
     6781 tcagctcctt ccggtgggcg cggggcatga ctagttgcat gtggcaaact tctggttccc
     6841 aactggcgcc caaatggcgg acatgtcgac ggcggcgcct caatgtcagg tcattaaagt
     6901 ttagtgggcg attttgcata gcgttctgct gagccaccca aaatcgcgcc aagtaagaaa
     6961 gccggcaaaa ggataccgaa agtgcagcag acgttttgct catgccacgc ccacactctt
     7021 aggctctcgc ccattcccaa aaactttggc cgaagctcat caagccgaaa aagttttgcc
     7081 aacgataaat caaaattttt ttggatgctt gttaactcgt tcgtttaaat taagttgaac
     7141 attaaggcgc aaactgcttg ctaaaatttt atgtaatttt aagaatttcc tgctggcaaa
     7201 gaaaattgtg tggaaatgcg gcgtcgcggc tgacagctaa tgaaaatatt tgccggaaag
     7261 ggcggtgaaa attcttgagg gttttgcaat tagcagagtg ttcaactggc cttgactgcg
     7321 cattaataaa tatttcaagc aattgttaat ggttttggaa atcttaaagt tcgtgttaat
     7381 aagtcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     7441 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7501 gcggtatcag ctcactcaaa ggc
//
