LOCUS       Exported               10065 bp ds-DNA     circular SYN 28-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 28, 2019 from SnapGene 4.3.5
            https://www.snapgene.com
COMMENT     LOCUS pAC-Kozak-Gal4-3xFlag- 10065 bp ds-DNA circular 24-AUG-2017 
            DEFINITION .
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1479
                     /label=Spacer
     misc_feature    1929..1950
                     /label=pAcM_seq_f
     misc_feature    2085..2095
                     /label=Untitled
     misc_feature    2096..2100
                     /label=Kozak
     CDS             2101..2541
                     /label=Gal4 DNA binding domain
     misc_feature    2111..2117
                     /label=SV40 polyA
     misc_feature    complement(2117)
                     /label=pAcM_seq_r
     misc_feature    2542..2565
                     /label=2xGGGS linker
     misc_feature    2542..2554
                     /label=GTTGGAGGCGGATCAG
     misc_feature    2566..2631
                     /label=FLAG
     misc_feature    2660
                     /label=Spacer
     misc_feature    complement(2661..2754)
                     /label=SV40 polyA
     misc_feature    2755..5162
                     /label=selection-cassette
     misc_feature    complement(2755..2756)
                     /label=loxP
     misc_feature    2773..3414
                     /label=tj enhancer
     misc_feature    3427..3580
                     /label=DSCP Drosophila synthetic core promoter
     misc_feature    3453..3454
                     /label=1nt deletion - G
     misc_feature    3581..3612
                     /label=5UTR
     CDS             3613..4335
                     /label=mCherry
     misc_feature    4336..4389
                     /label=T2A
     CDS             4390..4989
                     /label=Puro codon juggled
     3'UTR           4993..5162
                     /label=armi 3'UTR
     misc_feature    5228..6308
                     /label=HR
     misc_feature    complement(6462..6479)
                     /label=CAGTGACGAAGGCTTGAG
     misc_feature    8693..8707
                     /label=Untitled
     misc_feature    8708..9713
                     /label=homology arm left
     misc_feature    8708..8737
                     /label=CATGGCAAAGATTACTCAGTTTTCTTTTGG
     misc_feature    complement(9690..9713)
                     /label=CTTGGGTCTGAGGTGGAAATGTGC
     misc_feature    9714..9728
                     /label=Untitled
ORIGIN
        1 tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc ggcgagtatt ttgaagaatg
       61 agaataattt gtatttatac gaaaatcagt tagtggaatt ttctacaaaa acatgttatc
      121 tatagataat tttgttgcaa aatatgttga ctatgacaaa gattgtatgt atataccttt
      181 aatgtattct cattttctta tgtatttata atggcaatga tgatactgat gatattttaa
      241 gatgatgcca gaccacaggc tgatttctgc gtcttttgcc gaacgcagtg catgtgcggt
      301 tgttgttttt tggaatagtt tcaattttcg gactgtccgc tttgatttca gtttcttggc
      361 ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat gcggcaaaac
      421 ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg gcgtggcggg
      481 gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca tgtgtgcgtg
      541 tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatc tcgctctctc
      601 tagcgccatc tcgtacgcat gctcaacgca ccgcatgttg ccgtgtcctt tatgcgtcat
      661 tttggctcga aataggcaat tatttaaaca aagattagtc aacgaaaacg ctaaaataaa
      721 taagtctaca atatggttac ttattgccat gtgtgtgcag ccaacgatag caacaaaagc
      781 aacaacacag tggctttccc tctttcactt tttgtttgca agcgcgtgcg agcaagacgg
      841 cacgaccggc aaacgcaatt acgctgacaa agagcagacg aagttttggc cgaaaaacat
      901 caaggcgcct gatacgaatg catttgcaat aacaattgcg atatttaata ttgtttatga
      961 agctgtttga cttcaaaaca cacaaaaaaa aaaataaaac aaattatttg aaagagaatt
     1021 aggaatcgga cagcttatcg ttacgggcta acagcacacc gagacgaaat agcttacctg
     1081 acgtcacagc ctctggaaga actgccgcca agcagagaga gagagaaaaa gagggagagc
     1141 agcttagacc gcatgtgctt gtgtgtgagg cgtctctctc ttcgtctcct gtttgcgcaa
     1201 acgcatagac tgcactgaga aaatcgatta cctatttttt atgaatgaat atttgcacta
     1261 ttactattca aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg
     1321 agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt
     1381 atagaaatgc ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt
     1441 taaaaccggc ttaagttagg tgtgttttct acgactagac tagtgatgcc cctactagaa
     1501 gatgtgtgtt gcacaaatgt ccctgaataa ccaatttgaa gtgcagatag cagtaaacgt
     1561 aagctaatat gaatattatt taactgtaat gttttaatat cgctggacat tactaataaa
     1621 cccactataa acacatgtac atatgtatgt tttggcatac aatgagtagt tggggaaaaa
     1681 atgtgtaaaa gcaccgtgac catcacagca taaagataac cagctgaagt atcgaatatg
     1741 agtaaccccc aaattgaatc acatgccgca actgatagga cccatggaag tacactcttc
     1801 atggcgatat acaagacaca cacaagcacg aacacccagt tgcggaggaa attctccgta
     1861 aatgaaaacc caatcggcga acaattcata cccatatatg gtaaaagttt tgaacgcgac
     1921 ttgagagcgg agagcattgc ggctgataag gttttagcgc taagcgggct ttataaaacg
     1981 ggctgcggga ccagttttca tatcactacc gtttgagttc ttgtgctgtg tggatactcc
     2041 tcccgacaca aagccgctcc atcagccagc agtcgtctaa tccagagacc ccggacaaac
     2101 atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
     2161 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
     2221 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
     2281 ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt
     2341 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
     2401 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
     2461 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
     2521 caaagacagt tgactgtatc gggaggcgga tcaggtggcg gtagcgacta caaagaccat
     2581 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gggtacgcgt
     2641 ggtaccgcta gctaggatcc gtttattgca gcttataatg gttacaaata aagcaatagc
     2701 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgttt atcaatcgat
     2761 aagcttgata tctcttaatg aagtacttta actgattgat aatttgaaca ttcgcaagtc
     2821 taagaatgca gtttttcata gtactccatt atgtcatgat gtattccatt atgtctcagg
     2881 ccacattcat atctgggctt tagaagctac cgagtaggtc tcagcttgca tcatagattt
     2941 ggggtctcat ctcgacgctt tccgagaagt ggattctctg tgtggtctgt cggcatggat
     3001 aaatgggcgt ccagcgatgc cgaccaccgc gggcaacagt ttaagaatca aaataaacac
     3061 ttcgatccct ccgggctccg ataacttctg tcaacaaact tgggtcgcgt aacggcgcat
     3121 gcaggagcac tggaggagta tgagctccag ccgaagtgca gcattggatt acaatcgtca
     3181 acatggagat cagcatcaga tcaatcgcca tctgccggcc cctaaaagaa agatactttg
     3241 gagcagtcga gctttcagtt ggggcggcgt aattcggcaa taatcagagg gccatatctg
     3301 catttccaag ccgccttgcc tgaaataaat cccttataaa aatcggttct tttagcctgt
     3361 ttgttttctt ttccattttg atttcacttc ttgcggtgtc gcccgcgacg ctatgcatct
     3421 gcgatcgagc tcgcccgggg atcgagcgca gcgtataaaa gggcgcgggg tggctgagag
     3481 catcagttgt gaatgaatgt tcgagccgag cagacgtgcc gctgccttcg ttaatatcct
     3541 ttgaataagc caactttgaa tcacaagacg cataccaaac ggcattccgg tactgttggt
     3601 aaagccacca tgatgccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     3661 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     3721 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     3781 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     3841 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     3901 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     3961 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4021 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4081 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4141 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4201 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     4261 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     4321 gacgagctgt acaaggaagg tcgtggatct cttctcactt gtggcgatgt tgaagagaat
     4381 cctggtccta tgacggaata taaaccgacc gtcaggttgg cgacgaggga tgatgtgccg
     4441 cgcgcggtca ggacgttggc ggcggccttt gcggattatc cggcgaccag gcatacggtg
     4501 gaccccgata ggcatataga aagagtgacg gaattacagg agttgttttt gaccagggtg
     4561 ggcttggata tagggaaagt ctgggtggcc gatgatgggg cggccgtcgc cgtgtggacg
     4621 acccccgaat cggtggaggc cggcgccgtc tttgcggaaa tagggcccag gatggcggaa
     4681 ctctcgggga gtagattagc ggcccaacag caaatggagg ggttgttagc cccccataga
     4741 ccgaaagaac cggcctggtt tttagcgacg gtgggggtga gcccggatca tcaagggaaa
     4801 gggttagggt cggcggtggt cttgccgggc gtcgaagccg cggaaagggc gggcgtcccg
     4861 gcgtttttag aaacgagtgc cccgaggaat ttgccgtttt atgaaagatt ggggtttacg
     4921 gtgacggcgg atgtggaagt cccggagggc cccaggacgt ggtgtatgac gaggaaaccg
     4981 ggggcgtgat gagcacatat gacagtttat tattaaatta aaacacttgt tattgatgaa
     5041 ttaaagtaac acaattaatc attaaacttg ttttaaatat ttgtacatat gtgctaattt
     5101 tagcgaatgg taacactccc aagcgcagtc tgggaacgct gctcgtttgg cttcgcagtg
     5161 tgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     5221 atgactaagg cgtgtacgtg ttgctgttct cctcctcaaa tcttctgccc gactcttact
     5281 caatctttcg ctctgtgagc tgtcattatt aatgcgtata attagcgcac ttgataaaga
     5341 ttgattgggg tggctttata tggggcagaa agggaaaaag gaaatacgcc agacatgaga
     5401 cgctcgcaca cagatgcaaa ccggaaacca cagagtaagc aaatacatac acgcacagat
     5461 tgcggcactt gtttatgtgg cacacgcatg acttgaactt aatttcctta ttttaatata
     5521 aacaacaaga gagtttatac cccctcttgt ttttgtcttc tcggaccagg actcgactag
     5581 acaaaaacca ggatatacag gcacgcagag aagattcacc caaataaagc aatcattgat
     5641 ataaagtatt ctcgaaggta tctactcggc ttacttgctg tttatctttc ggttttccca
     5701 gcaaacgttt tcttatgctt cagttgcata tttacgatta ttgtgctcac acatttcgtt
     5761 ttattcccga gcatatctag atttagttgg tactgctttg caccagcaga tcactctata
     5821 tcctgagtat aaaccacaga tttagggcta agctagtttc agatactcta ccgagtagca
     5881 gatgtgtttt ttgaactaaa aatttgtgtt atcaaagtaa aagcaatgag aaaattggcg
     5941 tggtattttg tgtttaggta caatagatta gtgtattata atattgtagg atatttgtga
     6001 gtctacgaag ctcaattctg acacatttgc tgtcttaagc gattttcttt gtaccattcc
     6061 cctagttatt tctatagctt tctttcagtc ccaccatatg gctcctactc atttcgagag
     6121 attcggttac tttcgggcct acacaaataa tccctgacca ggccaacata tgtgtttcct
     6181 gcggtttagt tgggatgttt tatggcgagt tcatttacac taatcatgca attcttgtta
     6241 cacttttatt ggcctctccc aaaagcctaa gcgggcttat tagcacgttg agtgcatggc
     6301 taatgagctc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     6361 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     6421 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     6481 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     6541 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     6601 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     6661 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gccagcaaaa
     6721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     6961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7801 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     7861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8101 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     8161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     8521 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     8581 aggaattaat tctatattct aaaaacacaa atgatacttc taaaaaaaat catgaatggc
     8641 atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact gtcacatcat
     8701 ttttccacat ggcaaagatt actcagtttt cttttgggtt tatatttttg aagtatcgaa
     8761 ttaactactg caactacata ttgtaaaata tttaaaaaca aaaaaacaaa aacactattg
     8821 tgggcaacac tcctcgtaat gccgaacatt taagccgtgt gtgctaaaga tccaaaaaaa
     8881 tttacttcta cagcgcggtt gacttttaac caaagaattt ataaataatg cgcaactgat
     8941 ttttaatttt ctcctttcgg tagtgtcgac agcagcgacg acagcaaatt attcgcgcgg
     9001 cagctgaaaa gggaagccaa aaccactgca gacccggtca gaaataaagt aatgtaaaac
     9061 tgctccacag gcatgcaagg acatcaacag gagctgcagg agcagcagtg gcagccacag
     9121 gcggagttgg ggaaaataac ttgcaattag ttcatgtcag gggctgtggc aagctatgga
     9181 gatactgctg ctggaaagtt aaagtcaaaa acccaaaaac attgtatgag gacaaagtga
     9241 catcaggtat gcaagtggaa gctttatttg tgtctgagat ttttcacttt ccccttgaac
     9301 ttgggctccg gtctagctta accctggcat tggtaaaatg tcgggttttc gagaaaggaa
     9361 acggaaattc tgtttggcta atgaaaatca atgaacgaat tttccttttc cattttctct
     9421 ctatcgtgta gaaaaaatgt gtctggatct aaattgagtc atcgagtttt cagatgaaac
     9481 gtgccaagta ccagagaaac ataataaaat atgctgcaca gttgttattc tttaaaataa
     9541 atttaagtct agaccacgct aaagctcgtt tatttgtcat gaaagctgtc ttgagtacat
     9601 ctaatctatg cataattatt atgaaaataa cccagcagaa aacacctgcg gttaagcaaa
     9661 aggcttaggg caaaaaagag aaaatctcag cacatttcca cctcagaccc aaggatctcg
     9721 ctgcctgtta tgtggcccac aaaccaagac acgttttatg gccattaaag ctggctgatc
     9781 gtcgccaaac accaaataca tatcaatatg tacattcgag aaagaagcga tcaaagaagc
     9841 gtcttcgggc gagtaggaga atgcggagga gaaggagaac gagctgatct agtatctctc
     9901 cacaatccaa tgccaactga ccaactggcc atattcggag caatttgaag ccaatttcca
     9961 tcgcctggcg atcgctccat tcttggctat atgtttttca ccgttcccgg ggccattttc
    10021 aaagactcgt cggtaagata agattgtgtc actcgctgtc tctct
//
