LOCUS       Exported                5284 bp ds-DNA     circular SYN 06-NOV-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Apr 24, 2019 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2171..2190)
                     /label=T3 Primer
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    2239..2379
                     /label=AAV-4 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     promoter        2400..2871
                     /label=U6
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=29
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     2400..2422
                     /label=#842 MH_U6_MluI_fw
     misc_feature    2627..2871
                     /label=short U6 Variant FZ
     promoter        2627..2867
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     2644..2675
                     /label=727
     misc_feature    2896..2982
                     /label=F+E Sp scaffold
                     /note="ApEinfo_fwdcolor=#0080c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2896..2982
                     /label=misc_feature
                     /note="3'overhang=GGCC
                     ugene_name=Fragment 3
                     5'overhang=GTAC
                     3'type=sticky
                     fragment_source=_0443 pBS H1 GFP
                     ugene_group=fragments
                     ApEinfo_fwdcolor=pink
                     3'strand=rev-compl
                     3'term=NotI
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     5'term=KpnI
                     5'strand=rev-compl
                     5'type=sticky"
     primer_bind     complement(2964..2988)
                     /label=#843 MH_SpScaffoldFE_XbaI_re
     polyA_signal    complement(2983..2988)
                     /label=6xT
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     polyA_site      complement(3009..3052)
                     /label=minimal polyA
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=26
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     CDS             complement(3059..3793)
                     /codon_start=1
                     /label=gfp
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
                     /translation="TGRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLR
                     QADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRLRPGAHHL
                     LQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAE
                     ERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRVAARQPLPEHPVRPEQRPQREARS
                     HGPAGVRDRRRDHSRHGRAVQVK"
     promoter        complement(3815..4436)
                     /label=RSV
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=29
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    complement(4455..4595)
                     /label=AAV-2 ITR
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     primer_bind     complement(4638..4660)
                     /label=T7 Primer
                     /note="ApEinfo_fwdcolor=pink
                     vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtatgcatc
     2221 ctgcaggacg cgtcctaccc actccctcta tgcgcgctcg ctcactcact cggccctgga
     2281 gaccaaaggt ctccagactg ccggcctctg gccggcaggg ccgagtgagt gagcgagcgc
     2341 gcatagaggg agtgggtacc tccatcatct aggtttgcca gatctgatat cggcgcgccc
     2401 gagtccaaca cccgtgggaa tcccatgggc accatggccc ctcgctccaa aaatgctttc
     2461 gcgtcgcgca gacactgctc ggtagtttcg gggatcagcg tttgagtaag agcccgcgtc
     2521 tgaaccctcc gcgccgcccc ggccccagtg gaaagacgcg caggcaaaac gcaccacgtg
     2581 acggagcgtg accgcgcgcc gagcgcgcgc caaggtcggg caggaagagg gcctatttcc
     2641 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa
     2701 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     2761 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     2821 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgagtc
     2881 cgagcagaag aagaagttta agagctatgc tggaaacagc atagcaagtt taaataaggc
     2941 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttgc ggccgcctcg
     3001 agtgatcaaa aaaccaacac acgcttccaa tgaaaataaa cgatccttta ttgctagcct
     3061 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     3121 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
     3181 gtggttgtcg ggcagcaaca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     3241 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     3301 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     3361 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     3421 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     3481 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     3541 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
     3601 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     3661 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     3721 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     3781 ggtggcgacc ggtggatccc gggcccgcgg tacccagctt ggaggtgcac accaatgtgg
     3841 tgaatggtca aatggcgttt attgtatcga gctaggcact taaatacaat atctctgcaa
     3901 tgcggaattc agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg
     3961 cacgatcgta ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta
     4021 aggcatgttg ctaactcatc cttaccatgt tgcaagacta ccaagagtat ttgcataaga
     4081 ctacatttcc ccctccctat gcaaaagcga aactactata tcctgagggg actcctaacc
     4141 gcgtacaacc gaagccccgc ttttcgccta aacacaccct agtcccctca gatacgcgta
     4201 tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg
     4261 caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg
     4321 acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt
     4381 gtactgagag tgcaccatac ggatctgcga tgataagctg tcaaacatga gaattggtcg
     4441 acgatatcag atctaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4501 ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc
     4561 ctcagtgagc gagcgagcgc gcagagaggg agtggccccc cccccccccc ccccctgcat
     4621 tctagagagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     4681 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4741 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4801 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     4861 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4921 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4981 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5041 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5101 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5161 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5221 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     5281 ttag
//
