LOCUS pCX_mTagBFP2_mT2 7501 bp ds-DNA circular 27-MAR-2019 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7501) AUTHORS . TITLE Direct Submission JOURNAL Exported Sunday, Mar 24, 2019 from SnapGene Viewer 4.3.4 https://www.snapgene.com COMMENT Created: Friday, August 26, 2005 5:19 PM pCAGGS sequence from LMBP Martine Vanhoucke ;Nucleotide sequence of pCAGGS Date: 21/02/1992 (Latest Update: 18/10/1999) ;Start of the nucleotide sequence in the middle of the first EcoRI site ;downstream from the AG promoter. ;(c) BCCM/LMBP Plasmid collection (Ghent University, Belgium). COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers enhancer 11..388 /note="hCMV" /locus_tag="hCMV" /label="hCMV" /ApEinfo_label="hCMV" /ApEinfo_fwdcolor="#5ac3fa" /ApEinfo_revcolor="#5ac3fa" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 670..762 /note="Exon 1?" /locus_tag="Exon 1?" /label="Exon 1?" /ApEinfo_label="Exon 1?" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" intron 763..1687 /note="intron" /locus_tag="intron" /label="intron" /ApEinfo_label="intron" /ApEinfo_fwdcolor="#eb606c" /ApEinfo_revcolor="#eb606c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1652..1672 /note="HN737" /locus_tag="HN737" /label="HN737" /ApEinfo_label="HN737" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1696..1715 /note="HN382" /locus_tag="HN382" /label="HN382" /ApEinfo_label="HN382" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1745..2455 /note="mTagBFP2" /locus_tag="mTagBFP2" /label="mTagBFP2" /ApEinfo_label="mTagBFP2" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1943..1951 /note="mTagBFP2 chromophore" /locus_tag="mTagBFP2 chromophore" /label="mTagBFP2 chromophore" /ApEinfo_label="mTagBFP2 chromophore" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2456..2530 /note="mGRASP T2A" /locus_tag="mGRASP T2A" /label="mGRASP T2A" /ApEinfo_label="mGRASP T2A" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2540..4519 /locus_tag="iCreERT2" /label="iCreERT2" /ApEinfo_label="iCreERT2" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 2543..3565 /codon_start=1 /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL S VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP R PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL G IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER W ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR Y LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED G D" /locus_tag="iCre" /label="iCre" /ApEinfo_label="iCre" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(2948..2967) /locus_tag="Rev primer" /label="Rev primer" /ApEinfo_label="Rev primer" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 3578..4513 /codon_start=1 /product="mutated ligand-binding domain of the human estrogen receptor (Feil et al., 1997)" /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPI L YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE I LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEE F VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQR L AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEE T DQSHLATAGSTSSHSLQKYYITGEAEGFPAT" /locus_tag="ERT2" /label="ERT2" /ApEinfo_label="ERT2" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" 3'UTR 4529..4620 /note="rabbit b-globin 3' UTR, polyA" /locus_tag="rabbit b-globin 3' UTR, polyA" /label="rabbit b-globin 3' UTR, polyA" /ApEinfo_label="rabbit b-globin 3' UTR, polyA" /ApEinfo_fwdcolor="#f76ca1" /ApEinfo_revcolor="#008040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" 3'UTR 4621..4977 /note="rabbit b-globin 3' flanking region" /locus_tag="rabbit b-globin 3' flanking region" /label="rabbit b-globin 3' flanking region" /ApEinfo_label="rabbit b-globin 3' flanking region" /ApEinfo_fwdcolor="#f76ca1" /ApEinfo_revcolor="#008040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter complement(5055..5137) /note="lac" /locus_tag="lac" /label="lac" /ApEinfo_label="lac" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5279..5354 /note="SV40 origin" /locus_tag="SV40 origin" /label="SV40 origin" /ApEinfo_label="SV40 origin" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 5385..5459 /note="SV40 early region polyA" /locus_tag="SV40 early region polyA" /label="SV40 early region polyA" /ApEinfo_label="SV40 early region polyA" /ApEinfo_fwdcolor="#ff3eee" /ApEinfo_revcolor="#ff3eee" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal complement(5385..5459) /note="SV40 late region polyA" /locus_tag="SV40 late region polyA" /label="SV40 late region polyA" /ApEinfo_label="SV40 late region polyA" /ApEinfo_fwdcolor="#ff3eee" /ApEinfo_revcolor="#ff3eee" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(5574..5975) /note="pMB1 origin" /locus_tag="pMB1 origin" /label="pMB1 origin" /ApEinfo_label="pMB1 origin" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(6516..7376) /note="bla" /locus_tag="bla" /label="bla" /ApEinfo_label="bla" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta 61 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 361 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact 421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt 481 tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc 541 gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc 601 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg 661 cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg 721 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc 781 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg 841 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg 901 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc 961 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc 1021 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg 1081 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc 1141 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt 1201 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg 1261 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg 1321 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg 1381 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg 1441 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat 1501 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg 1561 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt 1621 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt 1681 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt 1741 cgccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat 1801 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta 1861 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt 1921 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg 1981 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac 2041 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct 2101 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa 2161 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga 2221 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacgccaa 2281 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt 2341 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga 2401 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc ttaatggcgg 2461 ctccggcggc cgctccgagg gccgcggcag cctgctgaca tgcggcgacg tggaagagaa 2521 ccctggccct gtgggcagcg ccaacctgct gactgtgcac caaaacctgc ctgccctccc 2581 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca 2641 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg 2701 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct 2761 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct 2821 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct 2881 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct 2941 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg 3001 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc 3061 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat 3121 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct 3181 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa 3241 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg ccacctccca 3301 actgtccacc cgggccctgg aagggatctt tgaggccacc caccgcctga tctatggtgc 3361 caaggatgac tctgggcaga gatacctggc ctggtctggc cactctgcca gagtgggtgc 3421 tgccagggac atggccaggg ctggtgtgtc catccctgaa atcatgcagg ctggtggctg 3481 gaccaatgtg aacattgtga tgaactacat cagaaacctg gactctgaga ctggggccat 3541 ggtgaggctg ctagaggatg gggacctcga gccatctgct ggagacatga gagctgccaa 3601 cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct 3661 gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga 3721 gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc 3781 agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt 3841 gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg 3901 tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt 3961 ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc 4021 tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc 4081 tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga 4141 agagaaggac catatccacc gagtcctgga caagatcaca gacactttga tccacctgat 4201 ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat 4261 cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg 4321 caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca 4381 tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac 4441 tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg 4501 tttccctgcc acagcttgag gcgcgccgga tctttttccc tctgccaaaa attatgggga 4561 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc 4621 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca 4681 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc 4741 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt 4801 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt 4861 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt 4921 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct 4981 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 5041 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc 5101 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 5161 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta 5221 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 5281 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 5341 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag 5401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 5461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc 5521 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 5581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 5641 tcactcaaag gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg 5701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 6001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 6061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 6121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 6181 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 6241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 6301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 6481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 6541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 6601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 6661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 6721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 6781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 6841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 6901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 6961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 7021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 7081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 7141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 7201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 7261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 7321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 7381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 7441 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 7501 c //