LOCUS       pMC051-HisMS2_PLP_pac_L 4630 bp ds-DNA     circular     07-NOV-2019
DEFINITION  .
KEYWORDS    "Antibiotic Resistance"                                            
FEATURES             Location/Qualifiers
     RBS             415..437
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        2620..2638
                     /label="T7 promoter"
     misc_feature    1324..1324
                     /label="t->c (remove BsmBI)"
     rep_origin      complement(2928..3610)
                     /label="ColE1 origin"
     misc_feature    2658..2676
                     /label="c variant pac site"
     misc_feature    2639..2657
                     /label="Esp3I Cloning Site"
     misc_feature    3387..3387
                     /label="A->G (puc to ColE1)"
     CDS             complement(3705..4502)
                     /label="KanR"
     CDS             494..1675
                     /label="Maturation Protein"
     promoter        314..332
                     /label="T7 promoter"
     promoter        366..395
                     /label="lac promoter"
     misc_feature    1699..2499
                     /label="coat protein dimer"
     misc_feature    2034..2034
                     /label="c->t (remove BsaI)"
     misc_feature    2092..2094
                     /label="Start of second coat dimer"
     terminator      2677..2738
                     /label="T7 terminator"
     misc_feature    1078..1078
                     /label="g->a (remove BspMI)"
     misc_feature    2442..2442
                     /label="c->t (remove BsaI)"
     terminator      2513..2574
                     /label="T7 terminator"
     misc_feature    2131..2148
                     /label="His Tag"
     misc_feature    1699..2088
                     /label="Coat Protein"
     misc_feature    2575..2619
                     /label="Spacer"
     protein_bind    333..357
                     /label="lac operator"
     misc_feature    529..529
                     /label="g->a (remove BsaI)"
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGGCCGCT ACAGGGCGCT CCCATTCGCC ATTCAGGCTG CGCAACTGTT
      181 GGGAAGGGCG TTTCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT
      241 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
      301 ACGGCCAGTG AGGtaatacg actcactata ggggaattgt gagcggataa caattcccct
      361 ctagatttac actttatgct tccggctcgt atgtttctag agaattcaaa taattttgtt
      421 taactttaag aaggagattt aaatTGGCTA TCGCTGTAGG TAGCCGGAAT TCCATTCCTA
      481 GGAGGTTTGA CCTgtgcgag cttttagtac ccttgatagg gagaacgaaa ccttcgtccc
      541 ctccgttcgc gtttacgcgg acggtgagac tgaagataac tcattctctt taaaatatcg
      601 ttcgaactgg actcccggtc gttttaactc gactggggcc aaaacgaaac agtggcacta
      661 cccctctccg tattcacggg gggcgttaag tgtcacatcg atagatcaag gtgcctacaa
      721 gcgaagtggg tcatcgtggg gtcgcccgta cgaggagaaa gccggtttcg gcttctccct
      781 cgacgcacgc tcctgctaca gcctcttccc tgtaagccaa aacttgactt acatcgaagt
      841 gccgcagaac gttgcgaacc gggcgtcgac cgaagtcctg caaaaggtca cccagggtaa
      901 ttttaacctt ggtgttgctt tagcagaggc caggtcgaca gcctcacaac tcgcgacgca
      961 aaccattgcg ctcgtgaagg cgtacactgc cgctcgtcgc ggtaattggc gccaggcgct
     1021 ccgctacctt gccctaaacg aagatcgaaa gtttcgatca aaacacgtgg ccggcagatg
     1081 gttggagttg cagttcggtt ggttaccact aatgagtgat atccagggtg catatgagat
     1141 gcttacgaag gttcaccttc aagagtttct tcctatgaga gccgtacgtc aggtcggtac
     1201 taacatcaag ttagatggcc gtctgtcgta tccagctgca aacttccaga caacgtgcaa
     1261 catatcgcga cgtatcgtga tatggtttta cataaacgat gcacgtttgg catggttgtc
     1321 gtccctaggt atcttgaacc cactaggtat agtgtgggaa aaggtgcctt tctcattcgt
     1381 tgtcgactgg ctcctacctg taggtaacat gctcgagggc cttacggccc ccgtgggatg
     1441 ctcctacatg tcaggaacag ttactgacgt aataacgggt gagtccatca taagcgttga
     1501 cgctccctac gggtggactg tggagagaca gggcactgct aaggcccaaa tctcagccat
     1561 gcatcgaggg gtacaatccg tatggccaac aactggcgcg tacgtaaagt ctcctttctc
     1621 gatggtccat accttagatg cgttagcatt aatcaggcaa cggctctcta gatagAGCCC
     1681 TCAACCGGAG TTTGAAGCAT GGCTTCTAAC TTTACTCAGT TCGTTCTCGT CGACAATGGC
     1741 GGAACTGGCG ACGTGACTGT CGCCCCAAGC AACTTCGCTA ACGGGGTCGC TGAATGGATC
     1801 AGCTCTAACT CGCGTTCACA GGCTTACAAA GTAACCTGTA GCGTTCGTCA GAGCTCTGCG
     1861 CAGAATCGCA AATACACCAT CAAAGTCGAG GTGCCTAAAG TGGCAACCCA GACTGTTGGT
     1921 GGTGTAGAGC TTCCTGTAGC CGCATGGCGT TCGTACTTAA ATATGGAACT AACCATTCCA
     1981 ATTTTCGCTA CGAATTCCGA CTGCGAGCTT ATTGTTAAGG CAATGCAAGG TCTTCTAAAA
     2041 GATGGAAACC CGATTCCCTC AGCAATCGCA GCAAACTCCG GCATCTACGC TAACTTTACT
     2101 CAGTTCGTTC TCGTCGACAA TGGCGGTACC CATCACCATC ACCATCACGG TACCGGCGAC
     2161 GTGACTGTCG CCCCAAGCAA CTTCGCTAAC GGGGTCGCTG AATGGATCAG CTCTAACTCG
     2221 CGTTCACAGG CTTACAAAGT AACCTGTAGC GTtCGTCAGA GCTCTGCGCA GAATCGCAAA
     2281 TACACCATCA AAGTCGAGGT GCCTAAAGTG GCAACCCAGA CTGTTGGTGG TGTAGAGCTT
     2341 CCTGTAGCCG CATGGCGTTC GTACTTAAAT ATGGAACTAA CCATTCCAAT TTTCGCTACG
     2401 AATTCCGACT GCGAGCTTAT TGTTAAGGCA ATGCAAGGTC TTCTAAAAGA TGGAAACCCG
     2461 ATTCCCTCAG CAATCGCAGC AAACTCCGGC ATCTACTAAT AGACGCCGGC CAccgctgag
     2521 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgTCGAAC
     2581 AGAAAGTAAT CGTATTGTAC ACGGCCGCAT AATCGAAATT AATACGACTC ACTATAGGGA
     2641 GACGaggtca CGTCTCaACA TGAGGATCAC CCATGTccgc tgagcaataa ctagcataac
     2701 cccttggggc ctctaaacgg gtcttgaggg gttttttgTA ATTTCACTGC CCGCTTTCCA
     2761 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ACATGGTCAT AGCTGTTTCC TTGCGTATTG
     2821 GGCGCTCTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGG TAAAGCCTGG
     2881 GGTGCCTAAT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     2941 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     3001 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     3061 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     3121 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     3181 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     3241 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     3301 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     3361 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     3421 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     3481 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     3541 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     3601 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     3661 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTATTA GAAAAATTCA
     3721 TCCAGCAGAC GATAAAACGC AATACGCTGG CTATCCGGTG CCGCAATGCC ATACAGCACC
     3781 AGAAAACGAT CCGCCCATTC GCCGCCCAGT TCTTCCGCAA TATCACGGGT GGCCAGCGCA
     3841 ATATCCTGAT AACGATCCGC CACGCCCAGA CGGCCGCAAT CAATAAAGCC GCTAAAACGG
     3901 CCATTTTCCA CCATAATGTT CGGCAGGCAC GCATCACCAT GGGTCACCAC CAGATCTTCG
     3961 CCATCCGGCA TGCTCGCTTT CAGACGCGCA AACAGCTCTG CCGGTGCCAG GCCCTGATGT
     4021 TCTTCATCCA GATCATCCTG ATCCACCAGG CCCGCTTCCA TACGGGTACG CGCACGTTCA
     4081 ATACGATGTT TCGCCTGATG ATCAAACGGA CAGGTCGCCG GGTCCAGGGT ATGCAGACGA
     4141 CGCATGGCAT CCGCCATAAT GCTCACTTTT TCTGCCGGCG CCAGATGGCT AGACAGCAGA
     4201 TCCTGACCCG GCACTTCGCC CAGCAGCAGC CAATCACGGC CCGCTTCGGT CACCACATCC
     4261 AGCACCGCCG CACACGGAAC ACCGGTGGTG GCCAGCCAGC TCAGACGCGC CGCTTCATCC
     4321 TGCAGCTCGT TCAGCGCACC GCTCAGATCG GTTTTCACAA ACAGCACCGG ACGACCCTGC
     4381 GCGCTCAGAC GAAACACCGC CGCATCAGAG CAGCCAATGG TCTGCTGCGC CCAATCATAG
     4441 CCAAACAGAC GTTCCACCCA CGCTGCCGGG CTACCCGCAT GCAGGCCATC CTGTTCAATC
     4501 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     4561 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     4621 AAAGTGCCAC
//