LOCUS       Exported                9213 bp ds-DNA     circular SYN 10-JUN-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pCW57_CMV_ssRFPGFPKDEL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Hama
  TITLE     Direct Submission
  JOURNAL   Exported Monday, June 10, 2019 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    11..16
                     /label=XhoI
                     /note="color: #a6acb3"
     misc_feature    17..134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    188..193
                     /label=XhoI
                     /note="color: #a6acb3"
     protein_bind    202..472
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
                     /note="This feature has 13 segments:
                         1: 202 .. 220 / #31849b / tetO
                         2: 221 .. 243 / #31849b
                         3: 244 .. 262 / #31849b / tetO
                         4: 263 .. 285 / #31849b
                         5: 286 .. 304 / #31849b / tetO
                         6: 305 .. 327 / #31849b
                         7: 328 .. 346 / #31849b / tetO
                         8: 347 .. 369 / #31849b
                         9: 370 .. 388 / #31849b / tetO
                        10: 389 .. 411 / #31849b
                        11: 412 .. 430 / #31849b / tetO
                        12: 431 .. 453 / #31849b
                        13: 454 .. 472 / #31849b / tetO"
     promoter        505..543
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    637..642
                     /label=EcoRI
                     /note="color: #a6acb3"
     CDS             643..696
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /note="color: #993366"
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             697..1371
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP
                     /note="color: #ff00ff"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1420..2133
                     /codon_start=1
                     /label=EGFP
                     /note="color: #00ff00"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    2134..2145
                     /label=KDEL
                     /note="color: #00ff00"
     misc_feature    2134..2145
                     /note="MCS"
                     /note="color: #800080"
     misc_feature    2146..2148
                     /note="MCS"
                     /note="color: #800080"
     misc_feature    2149..2154
                     /label=BamHI
                     /note="color: #a6acb3"
     promoter        2155..2665
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2675..3271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             3272..3325
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 3322"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3326..4072
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /note="This feature has 8 segments:
                        1: 3326 .. 3943 / #993366 / rTetR
                        2: 3944 .. 3949 / #cc99b2
                        3: 3950 .. 3985 / #993366 / VP16 minimal transcriptio...
                        4: 3986 .. 3988 / #cc99b2
                        5: 3989 .. 4024 / #993366 / VP16 minimal transcriptio...
                        6: 4025 .. 4027 / #cc99b2
                        7: 4028 .. 4063 / #993366 / VP16 minimal transcriptio...
                        8: 4064 .. 4072 / #cc99b2"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(4530..4541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4529"
                     /translation="IEGR"
     LTR             4746..4979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5051..5172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5212..5347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        5347..5418
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5419..6279
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5419 .. 5487 / #ccffcc / signal sequence
                        2: 5488 .. 6279 / #ccffcc
                     Cleavage site after base 5487"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6450..7038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    7326..7347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        7362..7392
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 7362 .. 7367 / #ffffff / -35
                        2: 7368 .. 7385 / #ffffff
                        3: 7386 .. 7392 / #ffffff / -10"
     protein_bind    7400..7416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     7424..7440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        7461..7479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        7507..7733
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             7734..7914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    7961..8086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    8579..8812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
ORIGIN
        1 aattctcgac ctcgagtttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
       61 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      121 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcgaatcg
      181 atatgtcctc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      241 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      301 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      361 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      421 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      481 agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
      541 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      601 agaagacacc gggaccgatc cagcctccgc ggccccgaat tcatgaagtt atgcatatta
      661 ctggccgtcg tggcctttgt tggcctctcg ctcgggatgg cctcctccga ggacgtcatc
      721 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
      781 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      841 accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt ccagtacggc
      901 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      961 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     1021 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     1081 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     1141 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag
     1201 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag
     1261 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac
     1321 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc catcgatgaa
     1381 ttcactagtg ttaacggatc cctcgagcgg ccgcccatgg tgagcaaggg cgaggagctg
     1441 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1501 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1561 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1621 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1681 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1741 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1801 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1861 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1921 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1981 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2041 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2101 gggatcactc tcggcatgga cgagctgtac aagaaagatg agctctaagg atccggggtt
     2161 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     2221 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     2281 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     2341 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     2401 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     2461 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
     2521 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     2581 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     2641 ccgaatcacc gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg
     2701 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     2761 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     2821 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     2881 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     2941 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     3001 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     3061 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     3121 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     3181 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     3241 cacctggtgc atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg
     3301 tgatgttgaa gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg
     3361 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     3421 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     3481 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     3541 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     3601 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     3661 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     3721 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     3781 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     3841 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     3901 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     3961 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     4021 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     4081 ggatcgatcc aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4141 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4201 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4261 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4321 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4381 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4441 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4501 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4561 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4621 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc
     4681 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4741 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4801 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4861 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4921 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4981 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5041 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5101 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5161 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     5221 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5281 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5341 ggaggccttt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5401 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5461 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5521 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5581 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5641 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5701 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5761 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5821 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5881 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5941 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6001 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6061 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6121 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6181 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6241 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6301 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6361 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6421 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6481 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6541 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6601 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6661 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6721 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6781 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6841 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6901 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6961 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7021 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7081 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7141 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7201 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7261 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7321 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7381 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7441 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7501 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7561 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7621 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7681 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7741 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7801 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7861 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7921 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7981 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8041 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8101 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8161 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8221 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8281 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8341 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8401 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     8461 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8521 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8581 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8641 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8701 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8761 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8821 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8881 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8941 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9001 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9061 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9121 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9181 cgtttcagac ccacctccca accccgaggg gac
//
