LOCUS pMito-mCherry-FRB_IRES_FKBP(I)-GBPen.xdna 6432 bp DNA circular 31/05/2019 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=Ligation of : pMito-mCherry-FRB_IRES_FKBP(III)-GBPen.xdna [5 741 nt] : (BglII[3316] / HindIII[1969]) to PCR(pMito-mCherr y-FRB_IRES_FKBP(III)-GBPen.xdna) [683 nt] : (HindIII[11] / BglII[698]) COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers rep_origin 5708..6336 /label=ColE1 origin /SerialCloner_Color=&hFFFE0A /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True rep_origin 3358..3798 /label=F1 ori /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 4331..5122 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 4097..4293 /label=SV40 early promoter /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter 3865..3893 /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 4144..4221 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 616..714 /label=Tom70 /SerialCloner_Color=&h2529C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 2668..3033 /label=GBPen /SerialCloner_Color=&h0CC00E /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation"translation="AQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVSSHHHHHH" \ Misc 1742..2325 /label=IRES /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1438..1716 /label=FRB /SerialCloner_Color=&hC01BA3 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 727..1428 /label=mCherry /SerialCloner_Color=&hC00C12 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 1969..1974 /label=HindIII /SerialCloner_Color=&hD00000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True 1974..2656 /label=insert from PCR(pMito-mCherry-FRB_IRES_FKBP(III)-GBPen.xdna) /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from PCR(pMito-mCherry-FRB_IRES_FKBP(III)-GBPen.xdna) [683 nt] : (HindIII[11] / BglII[698]) \ misc_feature 2335..2655 /label=FKBP /SerialCloner_Color=&hC01DBB /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 181..183 /label=Frame1_mitoTrapStart? /SerialCloner_Color=&h8800C6 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 274..276 /label=Frame1_mitoTrapStart?_2 /SerialCloner_Color=&h8800C6 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 310..312 /label=Frame1_mitoTrapStart?_3 /SerialCloner_Color=&h8800C6 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 493..495 /label=Frame1_mitoTrapStart?_4 /SerialCloner_Color=&h8800C6 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 1372..1392 /label=AK010 5' Cherry-C1 SeqFP /SerialCloner_Color=&hFFFE0A /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature complement(3143..3161) /label=pEGFP_C2-RP SeqRP /SerialCloner_Color=&hFFFE0A /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 2656..2661 /label=BglIII /SerialCloner_Color=&hFB0106 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 ccggtggCCA CCATGAAGAG CTTCATTACA AGGAACAAGA CAGCCATTTT GGCAACCGTT 661 GCTGCTACAG GTACTGCCAT CGGTGCCTAC TATTATTACA ACCAATTGCA ACAGGATCCg 721 ggcggcGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC 781 AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG 841 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG 901 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG 961 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG 1021 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG 1081 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC 1141 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC 1201 GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC 1261 GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC 1321 GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC 1381 GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACac agggggaATC 1441 CTCTGGCATG AGATGTGGCA TGAAGGCCTG GAAGAGGCAT CTCGTTTGTA CTTTGGGGAA 1501 AGGAACGTGA AAGGCATGTT TGAGGTGCTG GAGCCCTTGC ATGCTATGAT GGAACGGGGC 1561 CCCCAGACTC TGAAGGAAAC ATCCTTTAAT CAGGCCTATG GTCGAGATTT AATGGAGGCC 1621 CAAGAGTGGT GCAGGAAGTA CATGAAATCA GGGAATGTCA AGGACCTCAC CCAAGCCTGG 1681 GACCTCTATT ATCATGTGTT CCGACGAATC TCAAAGtgaA AAGCGCagag aTCCCCGGGa 1741 tCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG GAATAAGGCC 1801 GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG 1861 CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC CCTCTCGCCA 1921 AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAa gctTCTTGAA 1981 GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT GGCGACAGGT 2041 GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA CAACCCCAGT 2101 GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA AGCGTATTCA 2161 ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT CTGGGGCCTC 2221 GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC 2281 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGggagtg 2341 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc 2401 gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga 2461 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg 2521 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat 2581 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cGATGTGGAG 2641 CTTCTAAAAC TGGAAagatc tggaggcgca CAGGTGCAGC TGGTGGAGTC TGGAGGAGCT 2701 CTGGTGCAGC CTGGAGGAAG CCTGCGCCTG AGCTGTGCAG CTAGCGGATT TCCTGTGAAC 2761 CGCTACAGCA TGCGCTGGTA CCGCCAGGCT CCTGGTAAAG AGCGCGAGTG GGTGGCTGGA 2821 ATGAGCAGCG CTGGAGATCG CAGCAGCTAC GAGGACAGCG TGAAAGGACG CTTTACAATC 2881 AGCCGCGATG ATGCTCGCAA CACAGTGTAC CTGCAGATGA ACTCTCTGAA ACCTGAGGAC 2941 ACTGCTGTGT ACTACTGTAA CGTGAACGTG GGTTTCGAGT ACTGGGGACA GGGAACACAG 3001 GTGACAGTGA GCTCTcacca tcaccatcac cattgaGGAG GCGCGGCGGC AGCagtactG 3061 aattCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA 3121 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC 3181 CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT 3241 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG 3301 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT AACGCGTAAA TTGTAAGCGT 3361 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA 3421 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT 3481 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG 3541 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT 3601 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC 3661 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG 3721 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT 3781 TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC 3841 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG 3901 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTCCTGAG GCGGAAAGAA CCAGCTGTGG 3961 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 4021 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 4081 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 4141 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 4201 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 4261 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC 4321 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 4381 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 4441 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 4501 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 4561 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 4621 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 4681 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 4741 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 4801 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 4861 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 4921 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 4981 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 5041 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 5101 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 5161 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC 5221 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG 5281 TTCTTCGCCC ACCCTAGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA 5341 ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT 5401 CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT 5461 TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA 5521 GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA 5581 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 5641 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 5701 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 5761 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 5821 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT 5881 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 5941 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 6001 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 6061 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 6121 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 6181 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 6241 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 6301 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 6361 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 6421 ACCGCCATGC AT //