LOCUS       Exported                8000 bp ds-DNA     circular SYN 31-MAY-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Gabor Balazsi
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 31, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     207..240
                     /label=CMV-SgrDI-mut-f
                     /note="color: black; sequence: 
                     CGCGATGTACGGGCCAGATCGTCGACGTTGACATT"
     promoter        232..904
                     /label=CMV-D2i Promoter
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     232..270
                     /label=CMV-D2i-SgrDI-f
                     /note="color: black; sequence: 
                     GATCGTCGACGGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     primer_bind     235..275
                     /label=SalI-CMV-D2ir-f
                     /note="color: black; sequence: 
                     TATGTCGACGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACG"
     primer_bind     complement(570..598)
                     /label=DC-2a-RFP-SH01_(pAAVS1)_rev
                     /note="3'Tm=56.1 3'Ta(annealing temp)=56.1"
                     /note="color: black; sequence: 
                     ggcgccACTAATACGTAGATGTACTGCCAAGTAGG"
     primer_bind     615..635
                     /label=CMV_Promoter_fwd
                     /note="3'Tm=56.1 3'Ta(annealing temp)=55.0"
                     /note="color: black; sequence: 
                     taggaacttcagatctGTGATGCGGTTTTGGCAGTAC"
     primer_bind     626..650
                     /label=TG1.8.2
                     /note="color: black; sequence: ttggcagtacatcaatgggcgtgga"
     primer_bind     733..760
                     /label=TG1.8.1
                     /note="color: black; sequence: 
                     actttccaaaatgtcgtaacaactccgc"
     primer_bind     complement(794..813)
                     /label=CMV_Promoter_rev
                     /note="3'Tm=55.0 3'Ta(annealing temp)=55.0"
                     /note="color: black; sequence: 
                     catggtggcaccggtAGCTCTGCTTATATAAACCTCCCAC"
     TATA_signal     804..810
                     /note="color: #a6acb3"
     protein_bind    814..832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     TATA_signal     833..839
                     /label=Initiator Element
                     /note="color: #a6acb3"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    1038..1046
                     /label=Beta-globin Donor Site
                     /note="color: #a6acb3"
     intron          1041..1613
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
                     /note="color: #------"
     misc_signal     1582
                     /label=Beta-Globin Intron Branch Point
                     /note="color: #a6acb3"
     misc_RNA        1602..1616
                     /label=Beta-globin Acceptor Site
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(1608..1630)
                     /label=TG1.13.4
                     /note="color: black; sequence: GGTGGCGCCTGCAGGACCTGTAG"
     primer_bind     1611..1651
                     /label=TG1.13.1
                     /note="color: black; sequence: 
                     caggtcctgcaggcgccaccATGGGTTCTAGACTGGACAAG"
     primer_bind     1626..1641
                     /label=TG1.4.9
                     /note="color: black; sequence: CAGTACggatccACCATGGGTTCTAG"
     CDS             1631..2254
                     /label=hTetR
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(2253..2287)
                     /label=TG1.4.10
                     /note="color: black; sequence: 
                     gccggcgccgctagcCTTTCTCTTCTTTTTTGGCC"
     primer_bind     2253..2287
                     /label=TG1.4.7
                     /note="color: black; sequence: 
                     GGCCAAAAAAGAAGAGAAAGgctagcggcgccggc"
     sig_peptide     2255..2272
                     /label=NLS
                     /note="color: #cc99b2; direction: RIGHT"
     gene            2271..2272
                     /gene="Med25VBD"
                     /label=Med25VBD
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2272..2287
                     /label=TG1.16.3
                     /note="color: black; sequence: 
                     TTGACCTTGACATGCTCCCCGGGgctagcggcgccggc"
     gene            2291..2713
                     /gene="LOVpep"
                     /label=LOVpep
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2300..2302
                     /gene="T406"
                     /note="color: #a6acb3"
     misc_feature    2303..2305
                     /gene="T407"
                     /note="color: #a6acb3"
     misc_feature    2330..2332
                     /gene="V416"
                     /note="color: #a6acb3"
     misc_feature    2678..2680
                     /gene="I532A"
                     /note="color: #a6acb3"
     primer_bind     complement(2698..2733)
                     /label=TG1.4.11
                     /note="color: black; sequence: 
                     gtactgACCGGTttaGGAGCCGCCGCCGGAGGGGGCGGCGAAGGCGTAGGCGTTCCAGG
                     TCCAggcggcctcgtcgatg"
     primer_bind     complement(2711..2747)
                     /label=TG1.13.3FIX
                     /note="color: black; sequence: 
                     TGAAGTTAGTAGCTCCGCTTCCgccgcggcggcgggc"
     primer_bind     complement(2711..2747)
                     /label=TG1.13.3
                     /note="color: black; sequence: 
                     TGAAGTTAGTAGCTCCGCTTCCttagccgcggcggcgggc"
     primer_bind     complement(2712..2733)
                     /label=TG1.4.8
                     /note="color: black; sequence: 
                     gtactgACCGGTttagccgcggcggcggg"
     misc_feature    2714..2725
                     /gene="degron"
                     /label=RRRG Degron
                     /note="color: #a6acb3"
     primer_bind     2726..2747
                     /label=TG1.13.5
                     /note="color: black; sequence: ggaagcggagctactaacttca"
     CDS             2735..2791
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 2788"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(2771..2791)
                     /label=TG1.7.6
                     /note="color: black; sequence: AGGTCCAGGGTTCTCCTCCAC"
     CDS             2792..3511
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2792 .. 2794 / #05fd14
                        2: 2795 .. 2797 / #05fd14 / 1a
                        3: 2798 .. 3511 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2792..2809
                     /label=HiFi-KRAS-GFP.fwd
                     /note="color: black; sequence: 
                     ctgctgaaaaATGGTGAGCAAGGGCGAG"
     primer_bind     2793..2812
                     /label=TG1.10.5
                     /note="color: black; sequence: TGGTGAGCAAGGGCGAGGAG"
     primer_bind     complement(2907..2928)
                     /label=TG1.10.2
                     /note="color: black; sequence: TTCAGGGTCAGCTTGCCGTAGG"
     primer_bind     2907..2928
                     /label=TG1.10.1
                     /note="color: black; sequence: CCTACGGCAAGCTGACCCTGAA"
     primer_bind     complement(3099..3119)
                     /label=TG1.10.4
                     /note="color: black; sequence: GGGTCTTGTAGTTGCCGTCGT"
     primer_bind     3099..3119
                     /label=TG1.10.3
                     /note="color: black; sequence: ACGACGGCAACTACAAGACCC"
     primer_bind     complement(3483..3508)
                     /label=hEGFP-STOPDEL-T2a-1stOL-r
                     /note="color: black; sequence: 
                     TGTTAGAAGACTTCCTCTGCCCTCCTTGTACAGCTCGTCCATGCCGAGAG"
     primer_bind     complement(3489..3510)
                     /label=TG1.10.6
                     /note="color: black; sequence: TACTTGTACAGCTCGTCCATGC"
     misc_feature    3542..3900
                     /label=WPRE
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    3958..4182
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(3979..4002)
                     /label=TG1.6.10
                     /note="color: black; sequence: cgggggaggggcaaacaacagatg"
     protein_bind    4466..4513
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(4479..4513)
                     /label=Upstream_FRT_Site_rev
                     /note="3'Tm=56.1 3'Ta(annealing temp)=55.7"
                     /note="color: black; sequence: 
                     aaccgcatcacagatctGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCG"
     primer_bind     complement(4479..4513)
                     /label=Downstream_FRT_Site_rev
                     /note="3'Tm=56.1 3'Ta(annealing temp)=55.7"
                     /note="color: black; sequence: 
                     gatgtcttgtcccccggaaggtcgacGAAGTTCCTATACTTTCTAGAGAATAGGAACTT
                     CG"
     CDS             4521..5543
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5673..5794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(5843..5859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5867..5883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5891..5921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5891 .. 5897 / #ffffff / -10
                        2: 5898 .. 5915 / #ffffff
                        3: 5916 .. 5921 / #ffffff / -35"
     protein_bind    5936..5957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6245..6833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7004..7864)
                     /label=AmpR
                     /note="color: #993366; direction: LEFT"
     promoter        complement(7865..7969)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcatccctat cagtgataga gatcagatct
      841 ccctatcagt gatagagagc tgttttgacc tccatagaag acaccgggac cgatccagcc
      901 tccggactct agcgtttaaa cttaagcttg gtacccgggg atcctctagg gcctctgagc
      961 tattccagaa gtagtgaaga ggcttttttg gaggcctagg cttttgcaaa aagctccgga
     1021 tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     1081 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     1141 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     1201 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     1261 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     1321 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     1381 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     1441 ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc
     1501 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     1561 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta caggtcctgc
     1621 aggcgccacc atgggttcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
     1681 actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     1741 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctggc
     1801 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     1861 agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg
     1921 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     1981 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     2041 cgtgggccac tttacactgg gctgcgtatt ggaggatcag gagcatcaag tagcaaaaga
     2101 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     2161 gttcgaccat cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     2221 cctggagaaa cagctaaagt gcgaaagcgg cgggccaaaa aagaagagaa aggctagcgg
     2281 cgccggcgcc tttttggcta ctacacttga acgtattgag aagaactttg tcattactga
     2341 cccaaggttg ccagataatc ccattatatt cgcgtccgat agtttcttgc agttgacaga
     2401 atatagccgt gaagaaattt tgggaagaaa ctgcaggttt ctacaaggtc ctgaaactga
     2461 tcgcgcgaca gtgagaaaaa ttagagatgc catagataac caaacagagg tcactgttca
     2521 gctgattaat tatacaaaga gtggtaaaaa gttctggaac ctctttcact tgcagcctat
     2581 gcgagatcag aagggagatg tccagtactt tattggggtt cagttggatg gaactgagca
     2641 tgtccgagat gctgccgaga gagagggcgt gatgctggcc aagaagaccg ccgagaacat
     2701 cgacgaggcc gcccgccgcc gcggcggaag cggagctact aacttcagcc tgctgaagca
     2761 ggctggagac gtggaggaga accctggacc tatggtgagc aagggcgagg agctgttcac
     2821 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2881 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2941 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3001 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3061 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3121 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3181 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3241 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3301 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3361 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3421 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3481 cactctcggc atggacgagc tgtacaagta aaccggtgcg gccgcaatca acctctggat
     3541 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3601 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3661 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3721 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3781 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3841 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3901 tccgtggtgt tgtcctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     3961 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4021 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4081 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4141 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4201 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4261 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4321 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4381 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4441 attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa
     4501 gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     4561 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     4621 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     4681 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     4741 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     4801 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     4861 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     4921 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     4981 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     5041 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     5101 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     5161 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     5221 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     5281 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     5341 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     5401 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     5461 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     5521 ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct
     5581 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5641 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5761 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5821 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6661 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7981 gaaaagtgcc acctgacgtc
//
