LOCUS       pAA41_parS-spacer_Piq-B 7114 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter/UTR         complement(6723..6827)
                          /label="catp, ChlR promoter / 5' UTR"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     CDS                  3354..4028
                          /label="mRFP1"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
     gene                 complement(6063..6827)
                          /label="Chlᴿ, chloramphenicol resistance"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     degron               4029..4070
                          /label="TS - ssrA_LAA"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     spacer               complement(114..3163)
                          /label="spacer from Ec.lacZ"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     protein binding site 10..25
                          /label="parS o1"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     replication origin   complement(4576..5905)
                          /label="pMB1 origin"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     protein binding site 68..83
                          /label="parS o2"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     promoter             3220..3272
                          /label="lacIq promoter"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     RBS                  3280..3356
                          /label="BCD07 Str4 fragment"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     protein binding site 1..90
                          /label="Cc.parS"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     terminator           4204..4298
                          /label="BBa_B0014"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     CDS                  complement(6063..6722)
                          /label="cat, chloramphenicol O-acetyltransferase A-1 (ChlR)"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     CDS                  4670..4861
                          /label="rop"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgctaatggt
      121 agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag tcgtcgccac
      181 caatccccat atggaaaccg tcgatattca gccatgtgcc ttcttccgcg tgcagcagat
      241 ggcgatggct ggtttccatc agttgctgtt gactgtagcg gctgatgttg aactggaagt
      301 cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc agaccgtttt
      361 cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag cggtcaaaac
      421 aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc cagtttaccc
      481 gctctgctac ctgcgccagc tggcagttca ggccaatccg cgccggatgc ggtgtatcgc
      541 tcgccacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc cggtaggttt
      601 tccggctgat aaataaggtt ttcccctgat gctgccacgc gtgagcggtc gtaatcagca
      661 ccgcatcagc aagtgtatct gccgtgcact gcaacaacgc tgcttcggcc tggtaatggc
      721 ccgccgcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct tcacttacgc
      781 caatgtcgtt atccagcggt gcacgggtga actgatcgcg cagcggcgtc agcagttgtt
      841 ttttatcgcc aatccacatc tgtgaaagaa agcctgactg gcggttaaat tgccaacgct
      901 tattacccag ctcgatgcaa aaatccattt cgctggtggt cagatgcggg atggcgtggg
      961 acgcggcggg gagcgtcaca ctgaggtttt ccgccagacg ccactgctgc caggcgctga
     1021 tgtgcccggc ttctgaccat gcggtcgcgt tcggttgcac tacgcgtact gtgagccaga
     1081 gttgcccggc gctctccggc tgcggtagtt caggcagttc aatcaactgt ttaccttgtg
     1141 gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc gccaccatcc
     1201 agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact tcgatggttt
     1261 gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc agcgctggat
     1321 gcggcgtgcg gtcggcaaag accagaccgt tcatacagaa ctggcgatcg ttcggcgtat
     1381 cgccaaaatc accgccgtaa gccgaccacg ggttgccgtt ttcatcatat ttaatcagcg
     1441 actgatccac ccagtcccag acgaagccgc cctgtaaacg gggatactga cgaaacgcct
     1501 gccagtattt agcgaaaccg ccaagactgt tacccatcgc gtgggcgtat tcgcaaagga
     1561 tcagcgggcg cgtctctcca ggtagcgaaa gccatttttt gatggaccat ttcggcacag
     1621 ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata tcggtggccg
     1681 tggtgtcggc tccgccgcct tcatactgca ccgggcggga aggatcgaca gatttgatcc
     1741 agcgatacag cgcgtcgtga ttagcgccgt ggcctgattc attccccagc gaccagatga
     1801 tcacactcgg gtgattacga tcgcgctgca ccattcgcgt tacgcgttcg ctcatcgccg
     1861 gtagccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg gtttcaatat
     1921 tggcttcatc caccacatac aggccgtagc ggtcgcacag cgtgtaccac agcggatggt
     1981 tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcatcagc aggatatcct
     2041 gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg tgacggttaa
     2101 cgcctcgaat cagcaacggc ttgccgttca gcagcagcag accattttca atccgcacct
     2161 cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt
     2221 caaccaccgc acgatagaga ttcgggattt cggcgctcca cagtttcggg ttttcgacgt
     2281 tcagacgtag tgtgacgcga tcggcataac caccacgctc atcgataatt tcaccgccga
     2341 aaggcgcggt gccgctggcg acctgcgttt caccctgcca taaagaaact gttacccgta
     2401 ggtagtcacg caactcgccg cacatctgaa cttcagcctc cagtacagcg cggctgaaat
     2461 catcattaaa gcgagtggca acatggaaat cgctgatttg tgtagtcggt ttatgcagca
     2521 acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc agataactgc
     2581 cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc
     2641 tcaggtcaaa ttcagacggc aaacgactgt cctggccgta accgacccag cgcccgttgc
     2701 accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg ccttcctgta
     2761 gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa
     2821 caaacggcgg attgaccgta atgggatagg tcacgttggt gtagatgggc gcatcgtaac
     2881 cgtgcatctg ccagtttgag gggacgacga cagtatcggc ctcaggaaga tcgcactcca
     2941 gccagctttc cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca
     3001 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     3061 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     3121 gacgttgtaa aacgacggcc agtgaatccg taatcatggt catccagttg gtctggtgtc
     3181 aaaaataaga agacaaaaat caccttgcgc taagagctcg acaccatcga atggtgcaaa
     3241 acctttcgcg gtatggcatg atagcgcccg gaagcatgcc acttaaaaag gagatcaaca
     3301 atgaaagcaa ttttcgtact gaaacatctt aatcatgctg gggagggttt ctaatggctt
     3361 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta
     3421 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga
     3481 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc
     3541 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc
     3601 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg
     3661 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta
     3721 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt
     3781 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa
     3841 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg
     3901 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct
     3961 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca
     4021 ccggtgctac ctccgcagcg aacgacgaaa attacgccct tgcagcgtaa taactacgtg
     4081 ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga tactatgact
     4141 gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt ccgcaccgct
     4201 gtctcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     4261 taaaaagcca gattattaat ccggcttttt tattatttcc tagctagcca acgcatatgg
     4321 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
     4381 tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg
     4441 ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg
     4501 agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc
     4561 cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat
     4621 cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca
     4681 ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga
     4741 gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca
     4801 cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     4861 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4921 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     4981 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     5041 agagtgcacc atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     5101 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5461 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5701 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     5761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5821 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5881 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5941 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6001 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc ttggtctgac
     6061 agttacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     6121 acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     6181 tcgccttgcg tataatattt gcccatcgtg aaaacggggg cgaagaagtt gtccatattg
     6241 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     6301 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     6361 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     6421 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     6481 tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg
     6541 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     6601 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     6661 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     6721 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     6781 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactt accaatgctg
     6841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6901 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     6961 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7021 agggttgagt gttgttccag tttggaacaa gagtccacct cgagctttca ggaaaatttt
     7081 tctgtataat agattcataa atttgagaga agag
//