LOCUS       pAA6_parS_Piq-BCD07-mRF 3480 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS                  207..283
                          /label="BCD07 Str4 fragment"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     gene                 complement(2429..3193)
                          /label="Chlᴿ, chloramphenicol resistance"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     protein binding site 1..90
                          /label="Cc.parS"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     terminator           1131..1225
                          /label="BBa_B0014"
                          /ApEinfo_revcolor=#808040
                          /ApEinfo_fwdcolor=#808040
     degron               956..997
                          /label="TS - ssrA_LAA"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     protein binding site 10..25
                          /label="parS o1"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     CDS                  complement(2429..3088)
                          /label="cat, chloramphenicol O-acetyltransferase A-1 (ChlR)"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     CDS                  281..955
                          /label="mRFP1"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
     replication origin   1599..2271
                          /label="pUC origin"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     protein binding site 68..83
                          /label="parS o2"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     promoter             147..199
                          /label="Piq, lacIq promoter"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     promoter/UTR         complement(3089..3193)
                          /label="catp, ChlR promoter / 5' UTR"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaagaaga
      121 caaaaatcac cttgcgctaa gagctcgaca ccatcgaatg gtgcaaaacc tttcgcggta
      181 tggcatgata gcgcccggaa gcatgccact taaaaaggag atcaacaatg aaagcaattt
      241 tcgtactgaa acatcttaat catgctgggg agggtttcta atggcttcct ccgaagacgt
      301 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      361 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      421 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      481 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      541 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      601 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      661 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      721 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      781 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      841 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      901 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgctacctc
      961 cgcagcgaac gacgaaaatt acgcccttgc agcgtaataa ctacgtgctg ctgaagttgc
     1021 ccgcaacaga gagtggaacc aaccggtgat accacgatac tatgactgag agtcaacgcc
     1081 atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgtc tcacactggc
     1141 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat
     1201 tattaatccg gcttttttat tatttcctaG CTAGCCAACG CATATGagac ggtcacagct
     1261 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     1321 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     1381 actatgcggc atcagagcag attgtactga gagtgcacca ttgcggtgtg aaataccgca
     1441 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     1501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagAac
     2101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2401 aacttggtct gacagcttgg tctgacagtt acgccccgcc ctgccactca tcgcagtact
     2461 gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct
     2521 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atcgtgaaaa
     2581 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     2641 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     2701 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     2761 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     2821 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     2881 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     2941 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     3001 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     3061 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     3121 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     3181 cgtgcccgat caacttacca atgctgtatt tagaaaaata aacaaatagg ggttccgcgc
     3241 acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt aaaattcgcg
     3301 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     3361 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     3421 ccacctcgag ctttcaggaa aatttttctg tataatagat tcataaattt gagagaagag
//