LOCUS       pCC48_parS-spacer_J2310 7887 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS                  4054..4130
                          /label="BCD07 Str4 fragment"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     CDS                  5444..5635
                          /label="rop"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     RBS                  complement(3350..3374)
                          /label="RBS TIR=1000"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     spacer               116..2676
                          /label="spacer from Ec.lacZ'"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     promoter             3994..4046
                          /label="Piq, lacIq promoter"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     promoter/UTR         complement(7497..7601)
                          /label="catp, ChlR promoter / 5' UTR"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     CDS                  complement(2750..3349)
                          /label="phlF"
                          /ApEinfo_revcolor=#75c6a9
                          /ApEinfo_fwdcolor=#75c6a9
     terminator           4978..5072
                          /label="BBa_B0014"
                          /ApEinfo_revcolor=#808040
                          /ApEinfo_fwdcolor=#808040
     protein binding site 1..90
                          /label="Cc.parS"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     protein binding site 10..25
                          /label="parS o1"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     degron               4803..4844
                          /label="TS - ssrA_LAA"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     CDS                  complement(6837..7496)
                          /label="cat, chloramphenicol O-acetyltransferase A-1 (ChlR)"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     replication origin   complement(5350..6679)
                          /label="pMB1 origin"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     protein binding site 68..83
                          /label="parS o2"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     gene                 complement(6837..7601)
                          /label="Chlᴿ, chloramphenicol resistance"
                          /ApEinfo_revcolor=#82ca9d
                          /ApEinfo_fwdcolor=#82ca9d
     spacer               3449..3962
                          /label="spacer from Ec.'lacZ"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     promoter             complement(3375..3410)
                          /label="BBa_J23100"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     CDS                  4128..4802
                          /label="mRFP1"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
     degron               complement(2708..2749)
                          /label="TS - ssrA_LAA"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaaatgac
      121 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      181 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      241 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
      301 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
      361 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
      421 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
      481 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
      541 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
      601 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
      661 aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga
      721 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
      781 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
      841 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
      901 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
      961 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     1021 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
     1081 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
     1141 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
     1201 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
     1261 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
     1321 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
     1381 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
     1441 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
     1501 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
     1561 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
     1621 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
     1681 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
     1741 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
     1801 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
     1861 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
     1921 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
     1981 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
     2041 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa
     2101 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
     2161 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
     2221 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
     2281 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
     2341 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
     2401 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
     2461 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
     2521 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
     2581 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
     2641 gtggcagcat caggggaaaa ccttatttat cagccgaaag tattcattat ggtgaagaca
     2701 cttattatca tgcagctaag gcgtagttct catcgtttgc cgcggaggta cgctgtgtac
     2761 ccggacaaac accattaatc agcaggaagg taaattcttc aatatcctgt tcaacggtca
     2821 gctgttcggt cagcaggcga taccaacaaa aaccaaaaat catatccagc agcagttcac
     2881 gattggtatc tttcggcagt tcaccattgc taatggcatt ttcaaccagt tttttcggca
     2941 tctcacgacg acgttccata aactgatctt tcagctgggt cagggttgca gggtccagct
     3001 gtgcttctgc aataacacaa cgaaatgctt caccacaaat ggtttcacgc caaactttcc
     3061 acagattacg cagcagaaaa tccagatcgg ctttaaagct acccagatcc ggaaatttac
     3121 gcacctgttc gctttcattt tcatacactt cggcaatcag tgctgcttta ttggtccacc
     3181 aacgataaat ggtcggtttg cttgcaccgg cacgacgtgc aacgctttca atgctcagac
     3241 cgctataacc acattctttc aggatttcaa tggtgctggt cagaattgct ttatgggtat
     3301 gcggactacg caggctacca atgctgctac ggctcggggt acgtgccatg ttatttacct
     3361 ctttacggtg tgattgctag cactgtacct aggactgagc tagccgtcaa ggtaccgata
     3421 agctgtcaaa catgagcaga tcctctacga aaacctaccg gattgatggt agtggtcaaa
     3481 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
     3541 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     3601 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     3661 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     3721 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
     3781 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
     3841 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
     3901 cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     3961 aagaagacaa aaatcacctt gcgctaagag ctcgacacca tcgaatggtg caaaaccttt
     4021 cgcggtatgg catgatagcg cccggaagca tgccacttaa aaaggagatc aacaatgaaa
     4081 gcaattttcg tactgaaaca tcttaatcat gctggggagg gtttctaatg gcttcctccg
     4141 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
     4201 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
     4261 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
     4321 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     4381 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     4441 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
     4501 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
     4561 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
     4621 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
     4681 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
     4741 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
     4801 ctacctccgc agcgaacgac gaaaattacg cccttgcagc gtaataacta cgtgctgctg
     4861 aagttgcccg caacagagag tggaaccaac cggtgatacc acgatactat gactgagagt
     4921 caacgccatg agcggcctca tttcttattc tgagttacaa cagtccgcac cgctgtctca
     4981 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
     5041 gccagattat taatccggct tttttattat ttcctagcta gccaacgcat atggcaaaac
     5101 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
     5161 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     5221 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
     5281 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
     5341 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
     5401 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
     5461 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     5521 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     5581 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5641 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5701 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     5761 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     5821 caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     5881 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5941 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6001 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6061 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6121 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6181 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6241 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6301 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6361 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6421 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6481 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6541 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6601 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6661 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6721 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6781 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagcttggtc tgacagttac
     6841 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     6901 aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     6961 tgcgtataat atttgcccat cgtgaaaacg ggggcgaaga agttgtccat attggccacg
     7021 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     7081 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     7141 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     7201 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     7261 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     7321 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     7381 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     7441 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     7501 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     7561 tcattatggt gaaagttgga acctcttacg tgcccgatca acttaccaat gctgtattta
     7621 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     7681 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     7741 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     7801 agtgttgttc cagtttggaa caagagtcca cctcgagctt tcaggaaaat ttttctgtat
     7861 aatagattca taaatttgag agaagag
//