LOCUS       pEE29_sopC-spacer_Piq-B 7078 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     replication origin 5981..6719
                        /label="CloDF13 origin"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     repeat             303..345
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     promoter           3699..3751
                        /label="lacIq promoter"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     repeat             217..259
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         4832..4885
                        /label="T7 TΦ terminator"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     repeat             174..216
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     repeat             88..130
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     repeat             432..474
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     repeat             45..87
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     misc_feature       1..475
                        /label="sopC (IncD)"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
     spacer             593..3642
                        /label="spacer from Ec.lacZ"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     repeat             389..431
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     CDS                3833..4546
                        /label="sfyfp"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     repeat             1..44
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     promoter/UTR       complement(5842..5936)
                        /label="AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     gene               complement(5025..5936)
                        /label="SpcR, spectinomycin-resistance"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     RBS                3759..3835
                        /label="BCD07 Str4 fragment"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     CDS                complement(5050..5841)
                        /label="aadA, aminoglycoside 3′′-adenylyltransferase"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     repeat             260..302
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     repeat             131..173
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     repeat             346..388
                        /label="IncD(sopC) iteron"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ggcctgaata ttctctctgg gcccactgtt ccacttgtat cgtcggtctg ataatcagac
       61 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac
      121 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga
      181 taatcagact gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca
      241 tggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg
      301 tcggtctgat tattagtctg gaaccacggt cccactcgta tcgtcggtct gattattagt
      361 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacgatccca
      421 ctcgtgttgt cggtctgatt atcggtctgg gaccacggtc ccacttgtat tgtcgatcag
      481 actatcagcg tgagactacg attccatcaa tgcctgtcaa gggcaagtat tgacatgtcg
      541 tcgtaacctg tagaacggag taacctcggt gcaagaagac aaaaatcacc ttatgaccat
      601 gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      661 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      721 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg
      781 gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga
      841 tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa
      901 cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg
      961 ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat
     1021 ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg
     1081 ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa
     1141 ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt tatctggaag atcaggatat
     1201 gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat
     1261 cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc
     1321 tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca
     1381 gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg
     1441 tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag
     1501 cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct
     1561 gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct
     1621 gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct
     1681 gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa
     1741 caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg
     1801 cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc
     1861 aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg
     1921 aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc
     1981 aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg
     2041 cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat
     2101 gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa
     2161 atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat
     2221 gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt
     2281 acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg
     2341 caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg
     2401 tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca
     2461 ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata
     2521 cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc
     2581 gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact
     2641 gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca
     2701 accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct
     2761 ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac
     2821 cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc
     2881 aggctttctt tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga
     2941 tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga
     3001 ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt
     3061 gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg
     3121 gcagcatcag gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg
     3181 tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat
     3241 tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc
     3301 gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt
     3361 gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg
     3421 cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta
     3481 cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac
     3541 atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc
     3601 agtatcggcg gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca
     3661 aaaataagaa gacaaaaatc accttgcgct aagagctcga caccatcgaa tggtgcaaaa
     3721 cctttcgcgg tatggcatga tagcgcccgg aagcatgcca cttaaaaagg agatcaacaa
     3781 tgaaagcaat tttcgtactg aaacatctta atcatgctgg ggagggtttc taatgcgtaa
     3841 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
     3901 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
     3961 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
     4021 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
     4081 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
     4141 cggcacgtac aaaacgcgtg cggaagtgaa atttgacggc gataccctgg taaaccgcat
     4201 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
     4261 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
     4321 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
     4381 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcta
     4441 ccaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
     4501 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaaacct ccgcagcgaa
     4561 cgacgaaaat tacgtgcgcc atcacgccgc gcatctcgcg ctccgacagc acgcgcagga
     4621 tgctgctgga tcttagtata ttagttaagt ataagaagga gatatacata tggcagatct
     4681 caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt ctggtaaaga
     4741 aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt
     4801 aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     4861 ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct
     4921 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
     4981 ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc ttgaacgaat
     5041 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     5101 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     5161 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     5221 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     5281 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     5341 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     5401 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     5461 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     5521 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     5581 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     5641 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     5701 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     5761 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     5821 ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5881 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaatagctag
     5941 ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa
     6001 gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa cagcagggcc
     6061 gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat aaacagacgc
     6121 ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag tacgggcgaa
     6181 acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct tgcgctctcc
     6241 tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac gggaagtgtg
     6301 gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt cgctccaagc
     6361 tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt
     6421 cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc attggtaact
     6481 gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa
     6541 gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg
     6601 gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt
     6661 cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6721 tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag
     6781 tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa
     6841 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
     6901 gagctaactt acattaattg cgttgcgccc agtttggaac aagagtccac ctcgagcttt
     6961 caggaaaatt tttctgtata atagattcat aaatttgaga gaagagtgcg accacgtttt
     7021 agtctacgtt tatctgtctt tacttaatgt cctttgttac aggccagaaa gcataact
//