LOCUS       pSM015_Para-lp-ecfp-par 4856 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter           1..72
                        /label="P.ara1"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                928..1812
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     operator           1..20
                        /label="araI1 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       62..67
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter/UTR       complement(2976..3106)
                        /label="blap, AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     misc_feature       39..44
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                complement(2115..2975)
                        /label="bla, β-lactamase"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     replication origin 3372..4212
                        /label="p15A/pACYC origin"
                        /ApEinfo_revcolor=#956a92
                        /ApEinfo_fwdcolor=#956a92
     CDS                103..153
                        /label="leader peptide"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     CDS                154..867
                        /label="ecfp BBa_E0020"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     terminator         1950..2044
                        /label="BBa_B0014"
                        /ApEinfo_revcolor=#808040
                        /ApEinfo_fwdcolor=#808040
     operator           24..38
                        /label="araI2 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     RBS                82..102
                        /label="RBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     gene               complement(2115..3106)
                        /label="AmpR, ampicillin-resistance"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     misc_feature       4477..4499
                        /label="lacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     linker             868..927
                        /label="linker2"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 catagcattt ttatccataa gattagcgga tcctaccttt tacttcttgc catcgactac
       61 ggatactgag cacatgcatg ccacttaaaa aggagatcaa caatgaaagc aattttcgta
      121 ctgaaacatc ttaatcatgc taaggagact agtatggtga gcaagggcga ggagctgttc
      181 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      241 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      301 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg
      361 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      421 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      481 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      541 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac
      601 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      661 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      721 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
      781 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      841 atcactctcg gcatggacga gctgtacaag agatctcgag ctcaagcttc gaattctgca
      901 gtcgacggta ccgcgggccc gggatccatg tccgaagggc gtcgtggtct gggtcgaggg
      961 ctttcggccc tgctgggcga ggtcgacgcc gcgccggctc aggcgccggg cgagcagctt
     1021 ggcggttccc gtgaagcgcc gatcgaaatc ctgcagcgca atcccgacca gccccgtcgg
     1081 accttccgcg aggaagacct cgaggatctg tcaaactcga tccgtgagaa gggcgttctg
     1141 cagccgatcc tggtgcggcc gtcgccggat acggctggcg agtaccagat cgtcgccggc
     1201 gagcgccgtt ggcgcgcagc ccagcgggcg ggcctcaaga ctgtgccgat catggtgcgc
     1261 gagctcgacg acctggcggt gctggagatc ggcatcatcg agaacgtcca gcgggctgat
     1321 ctgaacgtcc tggaagaagc gctctcctac aaggtgctga tggagaagtt cgagcgcacc
     1381 caggagaaca tcgcccagac catcggcaag agccgcagcc acgtggccaa cacgatgcgc
     1441 ttgctggccc taccggacga ggttcagtcg cacctggtca gtggtgagct gacggccggc
     1501 cacgcccgcg ccatcgctgc agcggctgac ccggtggccc tcgccaagca aatcatcgag
     1561 ggcggcttgt cggtgcgcga gaccgaggcc ctggcccgta aggcgcccaa cctgtctgcg
     1621 ggcaagagca aggggggccg ccccccacgc gtgaaggaca ccgacaccca ggcgctggag
     1681 tctgacctgt cctcggttct gggtctggat gtctcgatcg accatcgcgg gtctaccggc
     1741 acgctgacca tcacctacgc caccctcgag cagctcgacg acctctgcaa ccgactgacg
     1801 cgcgggatct gaaagcttgc tacgtgctgc tgaagttgcc cgcaacagag agtggaacca
     1861 accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct catttcttat
     1921 tctgagttac aacagtccgc accgctgtct cacactggct caccttcggg tgggcctttc
     1981 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2041 atttcctagc tagccgcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
     2101 ctgccttaaa aaaattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2881 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2941 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3001 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3061 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgtctcattt
     3121 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
     3181 tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc
     3241 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
     3301 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
     3361 gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag
     3421 tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga
     3481 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     3541 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
     3601 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     3661 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     3721 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     3781 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     3841 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     3901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
     3961 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
     4021 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     4081 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     4141 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca
     4201 gataaaatat ttgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     4261 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     4321 ggcgtagagg atctgctcat gtttgacagc ttatcgagct cagtgcgctc gagcttccca
     4381 accttaccag agggcgcccc agctggcaat tccactagtc tcgagcttcc caaccttacc
     4441 agagggcgcc ccagctggca attccgacgt ctgtgtggaa ttgtgagcgg ataacaattt
     4501 cacacagggc cctcggacac cgaggagaat gtcaagaggc gaacacacaa cgtcttggag
     4561 cgccagagga ggaacgagct aaaacggagc ttttttgccc tgcgtgacca gatcccggag
     4621 ttggaaaaca atgaaaaggc ccccaaggta gttatcctta aaaaagccac agcatacatc
     4681 ctgtccgtcc aagcagagga gcaaaagctc atttctgaag aggacttgtt gcggaaacga
     4741 cgagaacagt tgaaacacaa acttgaacag ctacggaact cttgtgcgta aggaaaagta
     4801 aggaaaacga ttccttctaa cagaaatgtc ctgagcaatc acctatgaac tgtcga
//