LOCUS       pSM040_parS_Piq-BCD07-m 4185 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     protein binding site 1..90
                          /label="Cc.parS"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     CDS                  complement(1275..1934)
                          /label="cat, chloramphenicol acetyltransferase"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     RBS                  207..283
                          /label="BCD07 Str4 fragment"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     replication origin   2085..3985
                          /label="pSC101 origin"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     Promoter/UTR         complement(1935..2039)
                          /label="catp, ChlR promoter / 5' UTR"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     terminator           1044..1104
                          /label="L3S2P21"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     gene                 complement(1275..2039)
                          /label="ChlR, chloramphenicol-resistance"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     terminator           complement(1162..1268)
                          /label="catt, ChlR terminator"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     promoter             147..199
                          /label="Piq, lacIq promoter"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     terminator           3990..4028
                          /label="BBa_B1006 "
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     protein binding site 10..25
                          /label="parS o1"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     protein binding site 68..83
                          /label="parS o2"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     degron               956..997
                          /label="TS - ssrA_LAA"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     terminator           4029..4077
                          /label="L3S3P22 47C>G"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     terminator           complement(1105..1157)
                          /label="L3S1P00"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     CDS                  281..955
                          /label="mRFP1"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaagaaga
      121 caaaaatcac cttgcgctaa gagctcgaca ccatcgaatg gtgcaaaacc tttcgcggta
      181 tggcatgata gcgcccggaa gcatgccact taaaaaggag atcaacaatg aaagcaattt
      241 tcgtactgaa acatcttaat catgctgggg agggtttcta atggcttcct ccgaagacgt
      301 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      361 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      421 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
      481 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      541 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      601 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      661 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      721 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      781 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      841 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      901 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgctacctc
      961 cgcagcgaac gacgaaaatt acgcccttgc agcgtaataa agagacctac tagtagcggc
     1021 cgctgcagtt aatcactgat taactcggta ccaaattcca gaaaagaggc ctcccgaaag
     1081 gggggccttt tttcgttttg gtccttttgt tatcaataaa aaaggggagc ggtttcccgc
     1141 tccccttatt gttcgtctac aaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1201 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1261 cgatatcatt taaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1321 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1381 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1441 ttgtccatat tggccacatt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
     1501 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1561 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1621 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1681 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1741 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1801 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1861 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1921 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1981 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
     2041 ttaaatgagt ttcagttcca caggtaccca gaaggtgctt ccaagttaca ttgtcgatct
     2101 gttcatggtg aacagctttg aatacaccaa aaactcgtaa aagctctgat gtatctatct
     2161 tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa
     2221 cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa
     2281 gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc
     2341 gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt
     2401 gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt
     2461 ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt
     2521 atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa
     2581 atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg
     2641 tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag
     2701 ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg
     2761 tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg
     2821 ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga
     2881 taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag
     2941 tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc
     3001 gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct actgatgttc
     3061 atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt
     3121 tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt
     3181 aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat
     3241 ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa
     3301 ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga
     3361 aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt
     3421 gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga
     3481 atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg
     3541 atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt
     3601 agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca
     3661 cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa
     3721 tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat
     3781 acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc
     3841 tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct ggccacttcg
     3901 gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat
     3961 caacaggctt acccgtctta ctgtcacgga aaaaaaaacc ccgcccctga cagggcgggg
     4021 ttttttttcc aattattgaa ggccgctaac gcggcctttt tttgtttctg gtctgcctta
     4081 atcaatgact aagaattcgc ggccgcttct agagggtctc ggagtccacc tcgagctttc
     4141 aggaaaattt ttctgtataa tagattcata aatttgagag aagag
//