LOCUS       pSPB845_PL-lacO1-rbs-lp 5351 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS                68..88
                        /label="RBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     terminator         3172..3232
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     CDS                1960..3045
                        /label="Ec.lacI"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     misc_feature       1912..1917
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter element   20..25
                        /label="-35"
                        /ApEinfo_revcolor=#fff79a
                        /ApEinfo_fwdcolor=#fff79a
     promoter element   26..42
                        /label="lacO1"
                        /ApEinfo_revcolor=#fff79a
                        /ApEinfo_fwdcolor=#fff79a
     CDS                914..1798
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     misc_feature       1889..1894
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter element   43..48
                        /label="-10"
                        /ApEinfo_revcolor=#fff79a
                        /ApEinfo_fwdcolor=#fff79a
     terminator         4285..4339
                        /label="tonBt (bidir), BBa_B0054"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     terminator         1806..1862
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     promoter           1..55
                        /label="BBa_R0011 PL-lacO1"
                        /ApEinfo_revcolor=#fff79a
                        /ApEinfo_fwdcolor=#fff79a
     linker             855..913
                        /label="linker2"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     CDS                140..854
                        /label="ecfp BBa_E0020"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     promoter element   3..19
                        /label="lacO1"
                        /ApEinfo_revcolor=#fff79a
                        /ApEinfo_fwdcolor=#fff79a
     terminator         5174..5212
                        /label="BBa_B1006 "
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     CDS                complement(3317..4132)
                        /label="aph(3′)-I / nptI / aphA1, aminoglycoside 3′-phosphotransferase type I, KanR"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     CDS                89..139
                        /label="leader peptide"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     gene               complement(3290..4280)
                        /label="Kanᴿ-I, kanamycin resistance"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     terminator         complement(3233..3285)
                        /label="L3S1P00"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         3050..3104
                        /label="L3S3P00 53C>G"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     replication origin complement(4344..5169)
                        /label="p15A/pACYC ori"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     promoter/UTR       complement(4133..4280)
                        /label="aphA1p, KanR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     misc_feature       1932..1959
                        /label="lacI 5′ UTR"
                        /ApEinfo_revcolor=#d6b295
                        /ApEinfo_fwdcolor=#d6b295
     promoter           1889..1923
                        /label="BBa_J23110"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         5213..5261
                        /label="L3S3P22 47C>G"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacacgaca
       61 tgcatgccac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga aacatcttaa
      121 tcatgctaag gagacttcaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      181 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      241 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      301 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg
      361 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      421 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      481 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      541 cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg tctatatcac
      601 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
      661 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      721 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      781 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      841 ggacgagctg tacaagagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
      901 gggcccggga tccatgtccg aagggcgtcg tggtctgggt cgagggcttt cggccctgct
      961 gggcgaggtc gacgccgcgc cggctcaggc gccgggcgag cagcttggcg gttcccgtga
     1021 agcgccgatc gaaatcctgc agcgcaatcc cgaccagccc cgtcggacct tccgcgagga
     1081 agacctcgag gatctgtcaa actcgatccg tgagaagggc gttctgcagc cgatcctggt
     1141 gcggccgtcg ccggatacgg ctggcgagta ccagatcgtc gccggcgagc gccgttggcg
     1201 cgcagcccag cgggcgggcc tcaagactgt gccgatcatg gtgcgcgagc tcgacgacct
     1261 ggcggtgctg gagatcggca tcatcgagaa cgtccagcgg gctgatctga acgtcctgga
     1321 agaagcgctc tcctacaagg tgctgatgga gaagttcgag cgcacccagg agaacatcgc
     1381 ccagaccatc ggcaagagcc gcagccacgt ggccaacacg atgcgcttgc tggccctacc
     1441 ggacgaggtt cagtcgcacc tggtcagtgg tgagctgacg gccggccacg cccgcgccat
     1501 cgctgcagcg gctgacccgg tggccctcgc caagcaaatc atcgagggcg gcttgtcggt
     1561 gcgcgagaca gaggccctgg cccgtaaggc gcccaacctg tctgcgggca agagcaaggg
     1621 gggccgcccc ccacgcgtga aggacaccga cacccaggcg ctggagtctg acctgtcctc
     1681 ggttctgggt ctggatgtct cgatcgacca tcgcgggtct accggcacgc tgaccatcac
     1741 ctacgccacc ctcgagcagc tcgacgacct ctgcaaccga ctgacgcgcg ggatctaata
     1801 atggcctcgg taccaaagac gaacaataag acgctgaaaa gcgtcttttt tcgttttggt
     1861 ccgctgcgtt gctagccctg caggcttctt tacggctagc tcagtcctag gtacaatgct
     1921 agctactcga cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
     1981 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
     2041 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
     2101 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
     2161 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
     2221 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
     2281 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
     2341 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
     2401 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
     2461 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
     2521 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
     2581 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
     2641 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
     2701 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggac
     2761 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
     2821 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     2881 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tttcactggt gaaaagaaaa
     2941 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3001 cagctggcac gacaggtttc ccgactggaa agcgggcagt aataatggcc caattattga
     3061 aggggagcgg gaaaccgctc cccttttttt gtttctggtc tgccgctggc tgccatggcg
     3121 tttgagacct gcaggtacta gtagcggccg ctgcagttaa tcactgatta actcggtacc
     3181 aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt ccttttgtta
     3241 tcaataaaaa aggggagcgg tttcccgctc cccttattgt tcgtctacag tgttacaacc
     3301 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     3361 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     3421 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     3481 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     3541 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     3601 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     3661 tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat
     3721 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     3781 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     3841 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     3901 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     3961 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     4021 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     4081 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc
     4141 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     4201 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt
     4261 ttgctgagtt gaaggatcag gagtcctgtt gagtaatagt caaaagcctc cggtcggagg
     4321 cttttgactt tctgcttacc caatagcgga gtgtatactg gcttactatg ttggcactga
     4381 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
     4441 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
     4501 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
     4561 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
     4621 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     4681 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
     4741 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
     4801 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     4861 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
     4921 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
     4981 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
     5041 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
     5101 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
     5161 tcttattaaa cggaaaaaaa aaccccgccc ctgacagggc ggggtttttt ttccaattat
     5221 tgaaggccgc taacgcggcc tttttttgtt tctggtctgc cttaatcaat gactaagaat
     5281 tcgcggccgc ttctagaggg tctcacttcc tcgagggcgc gcctgcaggc ttcggatccg
     5341 ctgcaggctt c
//