LOCUS       pSPB847_Para-BCD02-parB 3418 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature       45..50
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         1168..1228
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     replication origin complement(2435..3260)
                        /label="p15A/pACYC ori"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     promoter           1..78
                        /label="P.ara2"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     operator           30..44
                        /label="araI2 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     gene               complement(1286..2371)
                        /label="Ampᴿ, ampicillin resistance"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         3265..3303
                        /label="BBa_B1006 "
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         3304..3352
                        /label="L3S3P22 47C>G"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     promoter/UTR       complement(2241..2371)
                        /label="blap, AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     CDS                168..1052
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     terminator         2376..2430
                        /label="bidir. tonBt, BBa_B0054"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     operator           7..26
                        /label="araI1 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                complement(1380..2240)
                        /label="bla, β-lactamase (AmpR)"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     RBS                83..170
                        /label="BCD02 Str1"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     terminator         1060..1116
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     terminator         complement(1286..1379)
                        /label="blat, AmpR terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         complement(1229..1281)
                        /label="L3S1P00"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     misc_feature       68..73
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       complement(2699..2699)
                        /label="replication origin (predicted)"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 cgcacccata gcatttttat ccataagatt agcggatcct accttttact tcttgccatc
       61 gactacggat actgagcacg acgggcccaa gttcacttaa aaaggagatc aacaatgaaa
      121 gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg tccgaagggc
      181 gtcgtggtct gggtcgaggg ctttcggccc tgctgggcga ggtcgacgcc gcgccggctc
      241 aggcgccggg cgagcagctt ggcggttccc gtgaagcgcc gatcgaaatc ctgcagcgca
      301 atcccgacca gccccgtcgg accttccgcg aggaagacct cgaggatctg tcaaactcga
      361 tccgtgagaa gggcgttctg cagccgatcc tggtgcggcc gtcgccggat acggctggcg
      421 agtaccagat cgtcgccggc gagcgccgtt ggcgcgcagc ccagcgggcg ggcctcaaga
      481 ctgtgccgat catggtgcgc gagctcgacg acctggcggt gctggagatc ggcatcatcg
      541 agaacgtcca gcgggctgat ctgaacgtcc tggaagaagc gctctcctac aaggtgctga
      601 tggagaagtt cgagcgcacc caggagaaca tcgcccagac catcggcaag agccgcagcc
      661 acgtggccaa cacgatgcgc ttgctggccc taccggacga ggttcagtcg cacctggtca
      721 gtggtgagct gacggccggc cacgcccgcg ccatcgctgc agcggctgac ccggtggccc
      781 tcgccaagca aatcatcgag ggcggcttgt cggtgcgcga gacagaggcc ctggcccgta
      841 aggcgcccaa cctgtctgcg ggcaagagca aggggggccg ccccccacgc gtgaaggaca
      901 ccgacaccca ggcgctggag tctgacctgt cctcggttct gggtctggat gtctcgatcg
      961 accatcgcgg gtctaccggc acgctgacca tcacctacgc caccctcgag cagctcgacg
     1021 acctctgcaa ccgactgacg cgcgggatct aataatggcc tcggtaccaa agacgaacaa
     1081 taagacgctg aaaagcgtct tttttcgttt tggtccgctg cgtttgagac gtactagtag
     1141 cggccgctgc agttaatcac tgattaactc ggtaccaaat tccagaaaag aggcctcccg
     1201 aaaggggggc cttttttcgt tttggtcctt ttgttatcaa taaaaaaggg gagcggtttc
     1261 ccgctcccct tattgttcgt ctacagatta tcaaaaagga tcttcaccta gatcctttta
     1321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1381 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1441 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1501 agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac
     1561 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1621 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1681 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1741 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1801 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1861 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1921 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1981 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2041 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2101 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2161 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2221 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2341 ccgcgcacat ttccccgaaa agtgccacct ggagtcctgt tgagtaatag tcaaaagcct
     2401 ccggtcggag gcttttgact ttctgcttac ccaatagcgg agtgtatact ggcttactat
     2461 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     2521 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     2581 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     2641 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     2701 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     2761 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     2821 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     2881 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     2941 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3001 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     3061 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     3121 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     3181 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     3241 caagaagatc atcttattaa acggaaaaaa aaaccccgcc cctgacaggg cggggttttt
     3301 tttccaatta ttgaaggccg ctaacgcggc ctttttttgt ttctggtctg ccttaatcaa
     3361 tgactaagaa ttcgcggccg cttctagagc gtctcacttc ggatccgctg caggcttc
//