LOCUS       pSPB849_Para-rbs-GGGS-e 4187 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator         complement(2055..2148)
                        /label="blat, AmpR terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         1937..1997
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         3145..3199
                        /label="bidir. tonBt, BBa_B0054"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     linker             112..162
                        /label="M(GGGGS)₃S"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
     misc_feature       45..50
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     gene               complement(2055..3140)
                        /label="Ampᴿ, ampicillin resistance"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     operator           7..26
                        /label="araI1 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     operator           30..44
                        /label="araI2 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         4073..4121
                        /label="L3S3P22 47C>G"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     promoter           1..78
                        /label="P.ara2"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       68..73
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     replication origin complement(3204..4029)
                        /label="p15A/pACYC ori"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     linker             877..936
                        /label="linker2"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     CDS                complement(2149..3009)
                        /label="bla, β-lactamase (AmpR)"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         1829..1885
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     terminator         complement(1998..2050)
                        /label="L3S1P00"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     promoter/UTR       complement(3010..3140)
                        /label="blap, AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         4034..4072
                        /label="BBa_B1006 "
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     CDS                937..1821
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     CDS                163..876
                        /label="ecfp BBa_E0020"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     RBS                91..111
                        /label="RBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 cgcacccata gcatttttat ccataagatt agcggatcct accttttact tcttgccatc
       61 gactacggat actgagcacg acatgcatgc cacttaaaaa ggagatcaac aatgggtggg
      121 ggcggctcag gcggcggtgg ttccggtggt ggcggctctt caatggtgag caagggcgag
      181 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      241 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      301 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      361 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      421 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      481 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      541 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      601 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
      661 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
      721 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      781 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      841 gccgccggga tcactctcgg catggacgag ctgtacaaga gatctcgagc tcaagcttcg
      901 aattctgcag tcgacggtac cgcgggcccg ggatccatgt ccgaagggcg tcgtggtctg
      961 ggtcgagggc tttcggccct gctgggcgag gtcgacgccg cgccggctca ggcgccgggc
     1021 gagcagcttg gcggttcccg tgaagcgccg atcgaaatcc tgcagcgcaa tcccgaccag
     1081 ccccgtcgga ccttccgcga ggaagacctc gaggatctgt caaactcgat ccgtgagaag
     1141 ggcgttctgc agccgatcct ggtgcggccg tcgccggata cggctggcga gtaccagatc
     1201 gtcgccggcg agcgccgttg gcgcgcagcc cagcgggcgg gcctcaagac tgtgccgatc
     1261 atggtgcgcg agctcgacga cctggcggtg ctggagatcg gcatcatcga gaacgtccag
     1321 cgggctgatc tgaacgtcct ggaagaagcg ctctcctaca aggtgctgat ggagaagttc
     1381 gagcgcaccc aggagaacat cgcccagacc atcggcaaga gccgcagcca cgtggccaac
     1441 acgatgcgct tgctggccct accggacgag gttcagtcgc acctggtcag tggtgagctg
     1501 acggccggcc acgcccgcgc catcgctgca gcggctgacc cggtggccct cgccaagcaa
     1561 atcatcgagg gcggcttgtc ggtgcgcgag acagaggccc tggcccgtaa ggcgcccaac
     1621 ctgtctgcgg gcaagagcaa ggggggccgc cccccacgcg tgaaggacac cgacacccag
     1681 gcgctggagt ctgacctgtc ctcggttctg ggtctggatg tctcgatcga ccatcgcggg
     1741 tctaccggca cgctgaccat cacctacgcc accctcgagc agctcgacga cctctgcaac
     1801 cgactgacgc gcgggatcta ataatggcct cggtaccaaa gacgaacaat aagacgctga
     1861 aaagcgtctt ttttcgtttt ggtccgctgc gtttgagacg tactagtagc ggccgctgca
     1921 gttaatcact gattaactcg gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc
     1981 ttttttcgtt ttggtccttt tgttatcaat aaaaaagggg agcggtttcc cgctcccctt
     2041 attgttcgtc tacagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2281 gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3121 tccccgaaaa gtgccacctg gagtcctgtt gagtaatagt caaaagcctc cggtcggagg
     3181 cttttgactt tctgcttacc caatagcgga gtgtatactg gcttactatg ttggcactga
     3241 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
     3301 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
     3361 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
     3421 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
     3481 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     3541 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
     3601 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
     3661 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     3721 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
     3781 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
     3841 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
     3901 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
     3961 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
     4021 tcttattaaa cggaaaaaaa aaccccgccc ctgacagggc ggggtttttt ttccaattat
     4081 tgaaggccgc taacgcggcc tttttttgtt tctggtctgc cttaatcaat gactaagaat
     4141 tcgcggccgc ttctagagcg tctcacttcg gatccgctgc aggcttc
//