LOCUS       pSPB858_Para-BCD02-GCN4 3625 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator         1267..1323
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       68..73
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         1375..1435
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     linker             276..314
                        /label="G₃SG₄SG₃S linker"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     misc_feature       45..50
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                168..269
                        /label="Sc.GCN4 leucine zipper MSE mutant"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     RBS                83..170
                        /label="BCD02 Str1"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     operator           30..44
                        /label="araI2 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                complement(1587..2447)
                        /label="bla, β-lactamase (AmpR)"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     promoter/UTR       complement(2448..2578)
                        /label="blap, AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     operator           7..26
                        /label="araI1 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter           1..78
                        /label="P.ara2"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         3472..3510
                        /label="BBa_B1006 "
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         2583..2637
                        /label="bidir. tonBt, BBa_B0054"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     terminator         3511..3559
                        /label="L3S3P22 47C>G"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     CDS                375..1259
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     linker             315..374
                        /label="linker2"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     linker             270..275
                        /label="KR"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     terminator         complement(1493..1586)
                        /label="blat, AmpR terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     replication origin complement(2642..3467)
                        /label="p15A/pACYC ori"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     terminator         complement(1436..1488)
                        /label="L3S1P00"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     gene               complement(1493..2578)
                        /label="Ampᴿ, ampicillin resistance"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 cgcacccata gcatttttat ccataagatt agcggatcct accttttact tcttgccatc
       61 gactacggat actgagcacg acgggcccaa gttcacttaa aaaggagatc aacaatgaaa
      121 gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg tcggttaagg
      181 aactggaaga taaagtcgaa gaactgctga gcaaaaacta tcatctggaa aacgaagtgg
      241 cgcgcctgaa aaaactggtg ggcgaacgca agagaggcgg tggctctgga ggtggtgggt
      301 ccggcggtgg ttccaagaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
      361 cgggcccggg atccatgtcc gaagggcgtc gtggtctggg tcgagggctt tcggccctgc
      421 tgggcgaggt cgacgccgcg ccggctcagg cgccgggcga gcagcttggc ggttcccgtg
      481 aagcgccgat cgaaatcctg cagcgcaatc ccgaccagcc ccgtcggacc ttccgcgagg
      541 aagacctcga ggatctgtca aactcgatcc gtgagaaggg cgttctgcag ccgatcctgg
      601 tgcggccgtc gccggatacg gctggcgagt accagatcgt cgccggcgag cgccgttggc
      661 gcgcagccca gcgggcgggc ctcaagactg tgccgatcat ggtgcgcgag ctcgacgacc
      721 tggcggtgct ggagatcggc atcatcgaga acgtccagcg ggctgatctg aacgtcctgg
      781 aagaagcgct ctcctacaag gtgctgatgg agaagttcga gcgcacccag gagaacatcg
      841 cccagaccat cggcaagagc cgcagccacg tggccaacac gatgcgcttg ctggccctac
      901 cggacgaggt tcagtcgcac ctggtcagtg gtgagctgac ggccggccac gcccgcgcca
      961 tcgctgcagc ggctgacccg gtggccctcg ccaagcaaat catcgagggc ggcttgtcgg
     1021 tgcgcgagac agaggccctg gcccgtaagg cgcccaacct gtctgcgggc aagagcaagg
     1081 ggggccgccc cccacgcgtg aaggacaccg acacccaggc gctggagtct gacctgtcct
     1141 cggttctggg tctggatgtc tcgatcgacc atcgcgggtc taccggcacg ctgaccatca
     1201 cctacgccac cctcgagcag ctcgacgacc tctgcaaccg actgacgcgc gggatctaat
     1261 aatggcctcg gtaccaaaga cgaacaataa gacgctgaaa agcgtctttt ttcgttttgg
     1321 tccgctgcgt ttgagacgta ctagtagcgg ccgctgcagt taatcactga ttaactcggt
     1381 accaaattcc agaaaagagg cctcccgaaa ggggggcctt ttttcgtttt ggtccttttg
     1441 ttatcaataa aaaaggggag cggtttcccg ctccccttat tgttcgtcta cagattatca
     1501 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1561 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1621 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1681 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga cccacgctca
     1741 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1801 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1861 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1921 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1981 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2041 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2101 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2161 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2221 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2281 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2341 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2401 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2461 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2521 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgga
     2581 gtcctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tgcttaccca
     2641 atagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt
     2701 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat
     2761 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     2821 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     2881 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     2941 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3001 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     3061 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     3121 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     3181 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     3241 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     3301 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     3361 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     3421 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaaacg gaaaaaaaaa
     3481 ccccgcccct gacagggcgg ggtttttttt ccaattattg aaggccgcta acgcggcctt
     3541 tttttgtttc tggtctgcct taatcaatga ctaagaattc gcggccgctt ctagagcgtc
     3601 tcacttcgga tccgctgcag gcttc
//