LOCUS       pSPB862_Para-BCD02-DsRe 4159 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter/UTR       complement(2982..3112)
                        /label="blap, AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         4045..4093
                        /label="L3S3P22 47C>G"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     linker             843..848
                        /label="GlySer"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     operator           7..26
                        /label="araI1 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       68..73
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         1801..1857
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     terminator         4006..4044
                        /label="BBa_B1006 "
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     CDS                complement(2121..2981)
                        /label="bla, β-lactamase (AmpR)"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     promoter           1..78
                        /label="P.ara2"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     linker             849..908
                        /label="linker2"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     terminator         complement(1970..2022)
                        /label="L3S1P00"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         1909..1969
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     misc_feature       45..50
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         complement(2027..2120)
                        /label="blat, AmpR terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     replication origin complement(3176..4001)
                        /label="p15A/pACYC ori"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     RBS                83..168
                        /label="BCD02 Str1"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     CDS                168..842
                        /label="DsRed"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     operator           30..44
                        /label="araI2 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     gene               complement(2027..3112)
                        /label="Ampᴿ, ampicillin resistance"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     CDS                909..1793
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     terminator         3117..3171
                        /label="bidir. tonBt, BBa_B0054"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
ORIGIN
        1 cgcacccata gcatttttat ccataagatt agcggatcct accttttact tcttgccatc
       61 gactacggat actgagcacg acgggcccaa gttcacttaa aaaggagatc aacaatgaaa
      121 gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg gataatacag
      181 aagatgttat taaagaattt atgcgtttta aagttcgtat ggaaggttca gttaatggtc
      241 atgaatttga aattgaaggt gaaggtgaag gacgtccata tgaaggtaca caaacagcaa
      301 aattaaaagt tacaaaaggt ggtccattac catttgcatg ggatatttta tcaccacaat
      361 ttcaatatgg ttcaaaagtt tatgttaaac atccagcaga tattccagat tataagaaat
      421 tatcatttcc tgaaggtttt aaatgggaac gtgttatgaa ttttgaagat ggtggtgttg
      481 ttacagttac acaagattca tcattacaag atggttgttt tatctataaa gttaaattta
      541 ttggtgttaa ttttccatca gatggtccag ttatgcaaaa gaaaacaatg ggttgggaac
      601 catcaacaga acgtttatat ccacgtgatg gtgttttaaa aggtgaaatt cataaagcat
      661 tgaaattaaa agatggtggt cattatttag ttgaatttaa atcaatttat atggcaaaaa
      721 aaccagttca attaccaggt tattattatg ttgattcaaa attagatatt acatcacata
      781 atgaagatta tacaattgtt gaacaatatg aacgtacaga aggtcgtcat catttgtttt
      841 taggttccaa gagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
      901 cgggatccat gtccgaaggg cgtcgtggtc tgggtcgagg gctttcggcc ctgctgggcg
      961 aggtcgacgc cgcgccggct caggcgccgg gcgagcagct tggcggttcc cgtgaagcgc
     1021 cgatcgaaat cctgcagcgc aatcccgacc agccccgtcg gaccttccgc gaggaagacc
     1081 tcgaggatct gtcaaactcg atccgtgaga agggcgttct gcagccgatc ctggtgcggc
     1141 cgtcgccgga tacggctggc gagtaccaga tcgtcgccgg cgagcgccgt tggcgcgcag
     1201 cccagcgggc gggcctcaag actgtgccga tcatggtgcg cgagctcgac gacctggcgg
     1261 tgctggagat cggcatcatc gagaacgtcc agcgggctga tctgaacgtc ctggaagaag
     1321 cgctctccta caaggtgctg atggagaagt tcgagcgcac ccaggagaac atcgcccaga
     1381 ccatcggcaa gagccgcagc cacgtggcca acacgatgcg cttgctggcc ctaccggacg
     1441 aggttcagtc gcacctggtc agtggtgagc tgacggccgg ccacgcccgc gccatcgctg
     1501 cagcggctga cccggtggcc ctcgccaagc aaatcatcga gggcggcttg tcggtgcgcg
     1561 agacagaggc cctggcccgt aaggcgccca acctgtctgc gggcaagagc aaggggggcc
     1621 gccccccacg cgtgaaggac accgacaccc aggcgctgga gtctgacctg tcctcggttc
     1681 tgggtctgga tgtctcgatc gaccatcgcg ggtctaccgg cacgctgacc atcacctacg
     1741 ccaccctcga gcagctcgac gacctctgca accgactgac gcgcgggatc taataatggc
     1801 ctcggtacca aagacgaaca ataagacgct gaaaagcgtc ttttttcgtt ttggtccgct
     1861 gcgtttgaga cgtactagta gcggccgctg cagttaatca ctgattaact cggtaccaaa
     1921 ttccagaaaa gaggcctccc gaaagggggg ccttttttcg ttttggtcct tttgttatca
     1981 ataaaaaagg ggagcggttt cccgctcccc ttattgttcg tctacagatt atcaaaaagg
     2041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2221 gagggcttac catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct
     2281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3001 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3061 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tggagtcctg
     3121 ttgagtaata gtcaaaagcc tccggtcgga ggcttttgac tttctgctta cccaatagcg
     3181 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     3241 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     3301 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     3361 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     3421 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     3481 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3541 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     3601 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     3661 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     3721 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     3781 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     3841 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     3901 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     3961 acgcgcagac caaaacgatc tcaagaagat catcttatta aacggaaaaa aaaaccccgc
     4021 ccctgacagg gcggggtttt ttttccaatt attgaaggcc gctaacgcgg cctttttttg
     4081 tttctggtct gccttaatca atgactaaga attcgcggcc gcttctagag cgtctcactt
     4141 cggatccgct gcaggcttc
//