LOCUS       pSPB863_Para-rbs-lp-DsR 4154 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     linker             844..903
                        /label="linker2"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     CDS                163..837
                        /label="DsRed"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
     operator           30..44
                        /label="araI2 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                904..1788
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     terminator         4001..4039
                        /label="BBa_B1006 "
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         1904..1964
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     RBS                91..111
                        /label="RBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     terminator         1796..1852
                        /label=" L3S2P55"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     CDS                112..162
                        /label="leader peptide"
                        /ApEinfo_revcolor=#f8d3a9
                        /ApEinfo_fwdcolor=#f8d3a9
     promoter/UTR       complement(2977..3107)
                        /label="blap, AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         4040..4088
                        /label="L3S3P22 47C>G"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     linker             838..843
                        /label="GlySer"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
     misc_feature       45..50
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         complement(1965..2017)
                        /label="L3S1P00"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     replication origin complement(3171..3996)
                        /label="p15A/pACYC ori"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     terminator         complement(2022..2115)
                        /label="blat, AmpR terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         3112..3166
                        /label="bidir. tonBt, BBa_B0054"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     misc_feature       68..73
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                complement(2116..2976)
                        /label="bla, β-lactamase (AmpR)"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     gene               complement(2022..3107)
                        /label="Ampᴿ, ampicillin resistance"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     operator           7..26
                        /label="araI1 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     promoter           1..78
                        /label="P.ara2"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 cgcacccata gcatttttat ccataagatt agcggatcct accttttact tcttgccatc
       61 gactacggat actgagcacg acatgcatgc cacttaaaaa ggagatcaac aatgaaagca
      121 attttcgtac tgaaacatct taatcatgct aaggagactt caatggataa tacagaagat
      181 gttattaaag aatttatgcg ttttaaagtt cgtatggaag gttcagttaa tggtcatgaa
      241 tttgaaattg aaggtgaagg tgaaggacgt ccatatgaag gtacacaaac agcaaaatta
      301 aaagttacaa aaggtggtcc attaccattt gcatgggata ttttatcacc acaatttcaa
      361 tatggttcaa aagtttatgt taaacatcca gcagatattc cagattataa gaaattatca
      421 tttcctgaag gttttaaatg ggaacgtgtt atgaattttg aagatggtgg tgttgttaca
      481 gttacacaag attcatcatt acaagatggt tgttttatct ataaagttaa atttattggt
      541 gttaattttc catcagatgg tccagttatg caaaagaaaa caatgggttg ggaaccatca
      601 acagaacgtt tatatccacg tgatggtgtt ttaaaaggtg aaattcataa agcattgaaa
      661 ttaaaagatg gtggtcatta tttagttgaa tttaaatcaa tttatatggc aaaaaaacca
      721 gttcaattac caggttatta ttatgttgat tcaaaattag atattacatc acataatgaa
      781 gattatacaa ttgttgaaca atatgaacgt acagaaggtc gtcatcattt gtttttaggt
      841 tccaagagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
      901 tccatgtccg aagggcgtcg tggtctgggt cgagggcttt cggccctgct gggcgaggtc
      961 gacgccgcgc cggctcaggc gccgggcgag cagcttggcg gttcccgtga agcgccgatc
     1021 gaaatcctgc agcgcaatcc cgaccagccc cgtcggacct tccgcgagga agacctcgag
     1081 gatctgtcaa actcgatccg tgagaagggc gttctgcagc cgatcctggt gcggccgtcg
     1141 ccggatacgg ctggcgagta ccagatcgtc gccggcgagc gccgttggcg cgcagcccag
     1201 cgggcgggcc tcaagactgt gccgatcatg gtgcgcgagc tcgacgacct ggcggtgctg
     1261 gagatcggca tcatcgagaa cgtccagcgg gctgatctga acgtcctgga agaagcgctc
     1321 tcctacaagg tgctgatgga gaagttcgag cgcacccagg agaacatcgc ccagaccatc
     1381 ggcaagagcc gcagccacgt ggccaacacg atgcgcttgc tggccctacc ggacgaggtt
     1441 cagtcgcacc tggtcagtgg tgagctgacg gccggccacg cccgcgccat cgctgcagcg
     1501 gctgacccgg tggccctcgc caagcaaatc atcgagggcg gcttgtcggt gcgcgagaca
     1561 gaggccctgg cccgtaaggc gcccaacctg tctgcgggca agagcaaggg gggccgcccc
     1621 ccacgcgtga aggacaccga cacccaggcg ctggagtctg acctgtcctc ggttctgggt
     1681 ctggatgtct cgatcgacca tcgcgggtct accggcacgc tgaccatcac ctacgccacc
     1741 ctcgagcagc tcgacgacct ctgcaaccga ctgacgcgcg ggatctaata atggcctcgg
     1801 taccaaagac gaacaataag acgctgaaaa gcgtcttttt tcgttttggt ccgctgcgtt
     1861 tgagacgtac tagtagcggc cgctgcagtt aatcactgat taactcggta ccaaattcca
     1921 gaaaagaggc ctcccgaaag gggggccttt tttcgttttg gtccttttgt tatcaataaa
     1981 aaaggggagc ggtttcccgc tccccttatt gttcgtctac agattatcaa aaaggatctt
     2041 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2101 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2161 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2221 cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga
     2281 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2341 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2401 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2461 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2521 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2581 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2641 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2701 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2761 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2821 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2881 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2941 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3001 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3061 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctggag tcctgttgag
     3121 taatagtcaa aagcctccgg tcggaggctt ttgactttct gcttacccaa tagcggagtg
     3181 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg
     3241 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc
     3301 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa
     3361 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg
     3421 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct
     3481 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg
     3541 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     3601 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     3661 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     3721 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     3781 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     3841 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     3901 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
     3961 cagaccaaaa cgatctcaag aagatcatct tattaaacgg aaaaaaaaac cccgcccctg
     4021 acagggcggg gttttttttc caattattga aggccgctaa cgcggccttt ttttgtttct
     4081 ggtctgcctt aatcaatgac taagaattcg cggccgcttc tagagcgtct cacttcggat
     4141 ccgctgcagg cttc
//