LOCUS       pSPB866_Para-BCD02-GCN4 4336 bp ds-DNA     circular     18-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS                83..170
                        /label="BCD02 Str1"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
     terminator         complement(2204..2297)
                        /label="blat, AmpR terminator"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     gene               complement(2204..3289)
                        /label="Ampᴿ, ampicillin resistance"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     terminator         complement(2147..2199)
                        /label="L3S1P00"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     operator           30..44
                        /label="araI2 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     replication origin complement(3353..4178)
                        /label="p15A/pACYC ori"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     misc_feature       68..73
                        /label="-10"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     linker             276..314
                        /label="G₃SG₄SG₃S linker"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     CDS                1086..1970
                        /label="Cc.parB"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
     terminator         4183..4221
                        /label="BBa_B1006 "
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     operator           7..26
                        /label="araI1 (araC operator)"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     misc_feature       45..50
                        /label="-35"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     terminator         2086..2146
                        /label="L3S2P21"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     terminator         1978..2034
                        /label="L3S2P55"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
     linker             270..275
                        /label="KR"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     promoter/UTR       complement(3159..3289)
                        /label="blap, AmpR promoter / 5′ UTR"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     CDS                complement(2298..3158)
                        /label="bla, β-lactamase (AmpR)"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
     promoter           1..78
                        /label="P.ara2"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
     CDS                168..269
                        /label="Sc.GCN4 leucine zipper MSE mutant"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
     terminator         3294..3348
                        /label="bidir. tonBt, BBa_B0054"
                        /ApEinfo_revcolor=#f6989d
                        /ApEinfo_fwdcolor=#f6989d
     CDS                315..1029
                        /label="ecfp BBa_E0020"
                        /ApEinfo_revcolor=#94a9ff
                        /ApEinfo_fwdcolor=#94a9ff
     terminator         4222..4270
                        /label="L3S3P22 47C>G"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
     linker             1030..1085
                        /label="linker2"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 cgcacccata gcatttttat ccataagatt agcggatcct accttttact tcttgccatc
       61 gactacggat actgagcacg acgggcccaa gttcacttaa aaaggagatc aacaatgaaa
      121 gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg tcggttaagg
      181 aactggaaga taaagtcgaa gaactgctga gcaaaaacta tcatctggaa aacgaagtgg
      241 cgcgcctgaa aaaactggtg ggcgaacgca agagaggcgg tggctctgga ggtggtgggt
      301 ccggcggtgg ttccgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      361 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      421 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      481 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg
      541 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      601 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      661 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      721 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca
      781 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg
      841 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      901 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
      961 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1021 tgtacaagag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
     1081 gatccatgtc cgaagggcgt cgtggtctgg gtcgagggct ttcggccctg ctgggcgagg
     1141 tcgacgccgc gccggctcag gcgccgggcg agcagcttgg cggttcccgt gaagcgccga
     1201 tcgaaatcct gcagcgcaat cccgaccagc cccgtcggac cttccgcgag gaagacctcg
     1261 aggatctgtc aaactcgatc cgtgagaagg gcgttctgca gccgatcctg gtgcggccgt
     1321 cgccggatac ggctggcgag taccagatcg tcgccggcga gcgccgttgg cgcgcagccc
     1381 agcgggcggg cctcaagact gtgccgatca tggtgcgcga gctcgacgac ctggcggtgc
     1441 tggagatcgg catcatcgag aacgtccagc gggctgatct gaacgtcctg gaagaagcgc
     1501 tctcctacaa ggtgctgatg gagaagttcg agcgcaccca ggagaacatc gcccagacca
     1561 tcggcaagag ccgcagccac gtggccaaca cgatgcgctt gctggcccta ccggacgagg
     1621 ttcagtcgca cctggtcagt ggtgagctga cggccggcca cgcccgcgcc atcgctgcag
     1681 cggctgaccc ggtggccctc gccaagcaaa tcatcgaggg cggcttgtcg gtgcgcgaga
     1741 cagaggccct ggcccgtaag gcgcccaacc tgtctgcggg caagagcaag gggggccgcc
     1801 ccccacgcgt gaaggacacc gacacccagg cgctggagtc tgacctgtcc tcggttctgg
     1861 gtctggatgt ctcgatcgac catcgcgggt ctaccggcac gctgaccatc acctacgcca
     1921 ccctcgagca gctcgacgac ctctgcaacc gactgacgcg cgggatctaa taatggcctc
     1981 ggtaccaaag acgaacaata agacgctgaa aagcgtcttt tttcgttttg gtccgctgcg
     2041 tttgagacgt actagtagcg gccgctgcag ttaatcactg attaactcgg taccaaattc
     2101 cagaaaagag gcctcccgaa aggggggcct tttttcgttt tggtcctttt gttatcaata
     2161 aaaaagggga gcggtttccc gctcccctta ttgttcgtct acagattatc aaaaaggatc
     2221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2401 ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca
     2461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg agtcctgttg
     3301 agtaatagtc aaaagcctcc ggtcggaggc ttttgacttt ctgcttaccc aatagcggag
     3361 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     3421 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     3481 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     3541 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     3601 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     3661 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3721 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     3781 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     3841 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     3901 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     3961 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     4021 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     4081 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     4141 cgcagaccaa aacgatctca agaagatcat cttattaaac ggaaaaaaaa accccgcccc
     4201 tgacagggcg gggttttttt tccaattatt gaaggccgct aacgcggcct ttttttgttt
     4261 ctggtctgcc ttaatcaatg actaagaatt cgcggccgct tctagagcgt ctcacttcgg
     4321 atccgctgca ggcttc
//