LOCUS       pPEE19                  7724 bp    DNA     circular SYN 14-Mar-2019
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    1..750
                     /note="Safe Haven (CNAG_02589 side)"
     gene            complement(911..1768)
                     /note="bla"
     rep_origin      complement(1919..2586)
                     /note="pUC origin"
     gene            2928..3284
                     /note="lacZ"
     misc_feature    complement(2984..3091)
                     /note="MCS"
     regulatory      3421..4650
                     /note="TEF1 promoter"
     CDS             3421..6938
                     /note="amdS Cassette"
     CDS             4651..6297
                     /note="amdS"
     regulatory      <6298..6938
                     /note="ADE6 terminator"
     misc_feature    6945..7694
                     /note="Safe Haven (CNAG_02588 side)"
     misc_difference 7695..7722
                     /note="Linker sequence"
BASE COUNT      1965 A      1944 C      1875 G      1940 T         0 OTHER
ORIGIN      ?
        1 GGTCTTCCCA TACGAATGTT CCCCATACTC CCAATTGCAT GGCGCTCGGC ACGACTGAGC
       61 AGGGGGAAGA AACTGTCCTT CTGCGTGCAC CATGTCGCTG GATAACCCCA CCCAGAAAAC
      121 AAACCAATCA TCTACGGTGA CTGGATTGGG CCTCACGAAA GACGATCATA CTCAGTTCTC
      181 ATAGACTCAT ATATGCCCTT TATTATTAAA TAACATAGAT CAGTTTTCTC ATGGAAATTG
      241 TTACATGAGT CACAACCTTT CCGCCCAATC TGATAACAAT CTGTGGATCA GTTTCATGAA
      301 CTTGAAATGC CAGACAGTCT GTTTCGTAGA AGGAAGCGTT CAAGTTTTGA GGGTTTGAGG
      361 AGTTTGATGA TATAGTCATT ATGAGGACGA AAGGAACGAC TACACTTTTT GGCCTCAGCC
      421 ATGTTGAAAC ATCACAGAGA TCAATGAGGA GAGATAACGC ACACGATAAG GAGGCACAGG
      481 TCTTCGAAAA ACCTTTATTT ATTCACCCGT AAGATATGTC TCCCACTATC TCTCTACTGT
      541 CTACTACCCT GAGATCCAAC ATGGGACTGT CAATCCTGCA AGATCCTGAG AAAATAAGGT
      601 CAAGCTCTTG TAACGTAGCA GCAGGTTGGG AGGTAAAATA TGTTGCCAGA TGTAGGCCAA
      661 AGTAACAAAC AGCCTTTACC TCATCAGCAA CAAATCCATG TAATGTGTAA ACTTCTAGGG
      721 GGCATTAAGA GGCACCGCTT GAGATGTACG ATTTAAATAG GCCTGCCGCT ACAGGGCGCG
      781 TCGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA
      841 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA
      901 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT
      961 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA
     1021 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG
     1081 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC
     1141 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA
     1201 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT
     1261 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT
     1321 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT
     1381 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA
     1441 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT
     1501 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC
     1561 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA
     1621 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT
     1681 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA
     1741 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT
     1801 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA
     1861 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT
     1921 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
     1981 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
     2041 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA
     2101 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
     2161 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
     2221 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
     2281 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
     2341 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
     2401 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
     2461 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
     2521 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
     2581 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT
     2641 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA
     2701 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA
     2761 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA
     2821 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT
     2881 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA
     2941 CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG
     3001 CGGCCGCTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA TTCGATATCA AGCTTATCGA
     3061 TACCGTCGAC CTCGAGGGGG GGCCCGGTAC CCAATTCGCC CTATAGTGAG TCGTATTACG
     3121 CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC
     3181 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA
     3241 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGAAATTG TAAGCGTTAA
     3301 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC
     3361 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTCCATG
     3421 GCGGCCGCCA TTCTATGCTA TACGGTACAA GATTGTGGCT ACTATGCAAG CTCCATAGCG
     3481 AATGATCTTA CACTGACCTT GTCGACCGTT CTGAATGATT AACAATGGTC GCAAGTGTGG
     3541 TTGACCCACC TGCGGGCGCT TCTTAACTAT CTATCATCGG TATGTCAAGT ATCACTCCCT
     3601 TTAAACAAAA GAATAAGAGC ATAATACAGC AGTCTCATCA TGGCAACCCT TCGCGCAATG
     3661 TACAAAAAGC AGCCAGAAGC CCCCACGCAC AAGCAATAAT GTCGTGTTCT ACCCTGCCAT
     3721 GAACAAGCGT CATGGCCATT AGCGAACGCC CCAATATTCA GCCCCATTCG CCAAGCAAGT
     3781 TGCCTCGCCC CCTTCCCAGA AGATCATGGG CATCTCGTCT TCTTCTAATT TCGCATTTTG
     3841 TGAAGCTCCA TTCATGCACG TCGGCTTCTG GACGACTCTT ATCATATGCA TTGGCCTGCT
     3901 CGGTGTGAGG ATGAGCCACT CCCACATGTT TAGACGGCAG GGGCCTCTTG CCACCCGTTG
     3961 AATTTTTGGT GCGAAGACGT GCCTGGGAAC GACCTCCACT TTAAATTTCT CCGTTGTTGC
     4021 GGTCGCTCCA TCCTCCTCAC ATTTCTTGCC TCCTAAGTCC CATGTGCCAC ACTCGGTGAA
     4081 AAACGGAGTT TGATACGGAT GAAATTTCCA GTGAGCTTTT CACATTCTGG AGGTTTCGAA
     4141 AAAAATTGTG GGATTTTCTG AATTAATCCG AGAATCCGCC CTGCCCATCA ACCGGAAAGA
     4201 ATTAAATCCC TCGCACATTC CTTTCTCCCA CCACCTTCTT TATCTCTACA TTCTTTAACC
     4261 AAAACACCTA GGTGAGTACC CAAAGTCTTT TGACACCTTT GAAAATCCGC GCCAGTCGTA
     4321 GGGCACGTAA AACAATTCTC GCTGGTGGCT TCTGCAGGTG AAGTCGGGGC AGAGTGGAGC
     4381 TGAGCATGGT CGACCACCCA CATCATTCTG TCTTGTTTTT GGCGATGTCT TCCATGCTTT
     4441 TGCCCTCATC TGTTGCGTCG ACTGCAGAGT ACTGTTTTGC CACTACTGAG CTCAACATGG
     4501 GCTTGTGTGT GGTGAAGAAA AAAATTTGGT GGCTCTTGAA AATTTTCAAC CCATCGGATA
     4561 CATGCCCTTC CAACTCACAA CGCCCTAACA CTTTATTGTC CTATCATTGC CTCTTCGCAC
     4621 ATGCTAACGA TCTCCACAGA AAACTTCAAA ATGCCTCAAT CCTGGGAAGA ACTGGCCGCT
     4681 GATAAGCGCG CCCGCCTCGC AAAAACCATC CCTGATGAAT GGAAAGTCCA GACGCTGCCT
     4741 GCGGAAGACA GCGTTATTGA TTTCCCAAAG AAATCGGGGA TCCTTTCAGA GGCCGAACTG
     4801 AAGATCACAG AGGCCTCCGC TGCAGATCTT GTGTCCAAGC TGGCGGCCGG AGAGTTGACC
     4861 TCGGTGGAAG TTACGCTAGC ATTCTGTAAA CGGGCAGCAA TCGCCCAGCA GTTAACAAAC
     4921 TGCGCCCACG AGTTCTTCCC TGACGCCGCT CTCGCGCAGG CAAGGGAACT CGATGAATAC
     4981 TACGCAAAGC ACAAGAGACC CGTTGGTCCA CTCCATGGCC TCCCCATCTC TCTCAAAGAC
     5041 CAGCTTCGAG TCAAGGGCTA CGAAACATCA ATGGGCTACA TCTCATGGCT AAACAAGTAC
     5101 GACGAAGGGG ACTCGGTTCT GACAACCATG CTCCGCAAAG CCGGTGCCGT CTTCTACGTC
     5161 AAGACCTCTG TCCCGCAGAC CCTGATGGTC TGCGAGACAG TCAACAACAT CATCGGGCGC
     5221 ACCGTCAACC CACGCAACAA GAACTGGTCG TGCGGCGGCA GTTCTGGTGG TGAGGGTGCG
     5281 ATCGTTGGGA TTCGTGGTGG CGTCATCGGT GTAGGAACGG ATATCGGTGG CTCGATTCGA
     5341 GTGCCGGCCG CGTTCAACTT CCTGTACGGT CTAAGGCCGA GTCATGGGCG GCTGCCGTAT
     5401 GCAAAGATGG CGAACAGCAT GGAGGGTCAG GAGACGGTGC ACAGCGTTGT CGGGCCGATT
     5461 ACGCACTCTG TTGAGGACCT CCGCCTCTTC ACCAAATCCG TCCTCGGTCA GGAGCCATGG
     5521 AAATACGACT CCAAGGTCAT CCCCATGCCC TGGCGCCAGT CCGAGTCGGA CATTATTGCC
     5581 TCCAAGATCA AGAACGGCGG GCTCAATATC GGCTACTACA ACTTCGACGG CAATGTCCTT
     5641 CCACACCCTC CTATCCTGCG CGGCGTGGAA ACCACCGTCG CCGCACTCGC CAAAGCCGGT
     5701 CACACCGTGA CCCCGTGGAC GCCATACAAG CACGATTTCG GCCACGATCT CATCTCCCAT
     5761 ATCTACGCGG CTGACGGCAG CGCCGACGTA ATGCGCGATA TCAGTGCATC CGGCGAGCCG
     5821 GCGATTCCAA ATATCAAAGA CCTACTGAAC CCGAACATCA AAGCTGTTAA CATGAACGAG
     5881 CTCTGGGACA CGCATCTCCA GAAGTGGAAT TACCAGATGG AGTACCTTGA GAAATGGCGG
     5941 GAGGCTGAAG AAAAGGCCGG GAAGGAACTG GACGCCATCA TCGCGCCGAT TACGCCTACC
     6001 GCTGCGGTAC GGCATGACCA GTTCCGGTAC TATGGGTATG CCTCTGTGAT CAACCTGCTG
     6061 GATTTCACGA GCGTGGTTGT TCCGGTTACC TTTGCGGATA AGAACATCGA TAAGAAGAAT
     6121 GAGAGTTTCA AGGCGGTTAG TGAGCTTGAT GCCCTCGTGC AGGAAGAGTA TGATCCGGAG
     6181 GCGTACCATG GGGCACCGGT TGCAGTGCAG GTTATCGGAC GGAGACTCAG TGAAGAGAGG
     6241 ACGTTGGCGA TTGCAGAGGA AGTGGGGAAG TTGCTGGGAA ATGTGGTGAC TCCATAGAAG
     6301 GTTGAGCGCG TAATAGTGGG TTGGTCTTGT CTCTATCCAT TCATCTGTGA GACATACTGA
     6361 TTTCAACTTT TTCATCAGAT AATAAGGATG GTGTGAGATA AGCAAGGCTG GGTTATGAGG
     6421 TTTGGGACTA GATGGTGTTT CGCGTCTAAA GGTGAGTCTT CGTTGGTTCA ATCACATTGA
     6481 TCTGATCGCT GATCAATTGT CAGCCTCTGA ATGTGCTCTT GAAGAAAATG GTTGTCTGTA
     6541 AGAATCCAGA ACCAAAAACG GTCATTTCAA GTATGTATCC TCCGTGAAGT TCCTTTAGCT
     6601 CTTGCTAATA TTGGACTTTA GCGTGCTTTA GCCAAAGACA GGGTCGCGAG GTTAAGAGGA
     6661 TGGGCCAGGA AAAATCTAAG TTAGATTTGT CACACAAAGA AAAAGATTGT CTCAGTTTTG
     6721 GTTTTGTTTG GTTTGATTTA TGCATAGGAA ATATTTATGA TCGAAATCAC TCATTTCCTG
     6781 CTTCGTAAAT ATCATCTACT ATATGCTTCG TAAATGTCCA TCTCCTACAT ATTGTGGATA
     6841 TGATAAATGC AATCCTCTCG TCTACACTCT CGGCTTATGC CTTTGTCTTC TTTGCGCTCC
     6901 TTTTAGCCTT CTTTTTATTC CTAGTCTTCA AGCCCATCTA CGTAGCCCGA ATAGGTTGGG
     6961 AGGAAATTTC AGAGGCTTAT GTTGGTATCG GCTGACGTTG AGCTGGAAAA GAAAACATAG
     7021 CTCAAATTAC GATACTACAG TAATGCGAGA ATAATACTTC ATGATGACAA GCCGCTGCTG
     7081 CACTAGCAGT GCGTAAACTG CCTGCCTCAT CACCAGCTGC ATCGATCTCT CATGAGTCAT
     7141 GACTGACCCA GCGCTTCATG AAACGGCTTC TCCGTGCTGA CGGCATCCAC CTGTACAGGC
     7201 TGCCACGCAG CAGCATTTTA TTCCGTTATA AGCACTCTTT TCAATCCCTC TTCATCCGTC
     7261 ACCGCTGCTC AATTTCAGCT CAAGATCGGT CCCATCCAGT ACTGGCCACA TGTTGAGTGA
     7321 CGAACTCCCA GCCCCTGATT CACGATGCGG GTAGCTTTGT TCTTGATGCT TGAGACCCTT
     7381 TGGCAGGTGC CCGTCACTCT TCAGCTCTTG CGCTGATAAG TTTCATTTGT GAACCAAGAA
     7441 GAAGAGTTCT ACCATTTGCA GTGCGTATTT ATGAGAAGAA GCTGGTGGCC GTGTGGTGCA
     7501 CTTGCTGGCT ATTGCCGGCT TGTTGCAGAT AAAGCCACAA GTAAGAAAGA TGGAGCCACC
     7561 ATGGCTGCAT CCCGATTGTC TGTGGCGGTA AGCCATTGAT GGGATGGAGA TAGCACCTCA
     7621 CGGCTGTGAA CGATCGACGG ATGACAAGAC GGAACTCCTC TTGCAGAAAG TGCGTGACGA
     7681 GTACTCGAAT ACTGTTAATT AACACAAGTA TCGTGGCGCG CCAG
//
