LOCUS       pPEE23                  7724 bp    DNA     circular SYN 14-Mar-2019
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    1..750
                     /note="Safe Haven (CNAG_05557 side)"
     gene            complement(913..1770)
                     /note="bla"
     rep_origin      complement(1921..2588)
                     /note="pUC origin"
     gene            2930..3286
                     /note="lacZ"
     misc_feature    complement(2986..3093)
                     /note="MCS"
     regulatory      3423..4652
                     /note="TEF1 promoter"
     CDS             3423..6940
                     /note="amdS Cassette"
     CDS             4653..6299
                     /note="amdS"
     regulatory      <6300..6940
                     /note="ADE6 terminator"
     misc_feature    6947..7696
                     /note="Safe Haven (CNAG_05558 side)"
     misc_difference 7697..7724
                     /note="Linker sequence"
BASE COUNT      1944 A      1923 C      1909 G      1948 T         0 OTHER
ORIGIN      ?
        1 ATGTAAAATG GACCCTCGGA AACTGGATGT TAGCAAAAGA ATCGGACATC ATAGCTGCGT
       61 ATCTTTCCAG TTCAACCCCA GAAAGTATTG CTGGTGACGT TAGACGCCTC GTTATGCCCT
      121 ATCTCTATGT CCTCGAATCC CGTGCGGAAA GAGCAGGGAT ACCCTCGTCC AATCTTATTT
      181 CAGACTCACT CAACTCCACA ATACTTTCTC TTCCGCTCCG TTTGGCGCTA CCACTCTTTG
      241 AAGCATCCAA GGCTACTCTT CCCCCTTCTG AAAGAGTGAT ACGCAACGAT CTGGATGTGG
      301 CTCGATTGGC TCTTGCATGC TTGTATGGCA GTCAAGAAAG AGGCGGTGCC GTTTGGTCAA
      361 CGATGTCATC TATCTTTGAG TGTCTTCCTG TCTGGGAGCT GACTGGAGAA GACGCTGAGG
      421 ACGAGGAGCT TACAAGTACG ACTCTCGATT CCATCTCAAA TTTCCTTCGC CCTACATCTG
      481 CTTCCACATC ACCACCTTCT AAGAGCGATA TTCTTTTCTT TTTCCAGCCT TTGCCCTTCG
      541 CCTCTCTTTC TCGGGCTCTT GATATTCTTG ACGTCCAACT CGAAAGTGGA GAAATACTGG
      601 CCAGATGGGG TGTTGAGAAA CGACTAGGGG AGCTTCTAGG GATGACGGGG GATCAAAAAG
      661 GACAAATGGA GCTGGCAGAG AGATTAGTGA GACAAGGCGA AGCCTCAGTA GGGAAAGGGG
      721 GGGATGATCG CTGGCGCAGA TTATGGAACG ATTTAAATAG GCCTGCCGCT ACAGGGCGCG
      781 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA
      841 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
      901 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA
      961 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT
     1021 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG
     1081 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC
     1141 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT
     1201 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA
     1261 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT
     1321 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT
     1381 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT
     1441 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA
     1501 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA
     1561 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT
     1621 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC
     1681 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT
     1741 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA
     1801 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT
     1861 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC
     1921 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG
     1981 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT
     2041 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG
     2101 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG
     2161 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC
     2221 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA
     2281 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA
     2341 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC
     2401 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT
     2461 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG
     2521 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT
     2581 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC
     2641 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC
     2701 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT
     2761 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT
     2821 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT
     2881 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT
     2941 TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT
     3001 GGCGGCCGCT CTAGAACTAG TGGATCCCCC GGGCTGCAGG AATTCGATAT CAAGCTTATC
     3061 GATACCGTCG ACCTCGAGGG GGGGCCCGGT ACCCAATTCG CCCTATAGTG AGTCGTATTA
     3121 CGCGCGCTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA
     3181 ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG
     3241 CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGAAAT TGTAAGCGTT
     3301 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG
     3361 GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTCCA
     3421 TGGCGGCCGC CATTCTATGC TATACGGTAC AAGATTGTGG CTACTATGCA AGCTCCATAG
     3481 CGAATGATCT TACACTGACC TTGTCGACCG TTCTGAATGA TTAACAATGG TCGCAAGTGT
     3541 GGTTGACCCA CCTGCGGGCG CTTCTTAACT ATCTATCATC GGTATGTCAA GTATCACACC
     3601 CTTTAAACAA AAGAATAAGA GCATAATACA GCAGTCTCAT CATGGCAACC CTTCGCGCAA
     3661 TGTACAAAAA GCAGCCAGAA GCCCCCACGC ACAAGCAATA ATGTCGTGTT CTACCCTGCC
     3721 ATGAACAAGC GTCATGGCCA TTAGCGAACG CCCCAATATT CAGCCCCATT CGCCAAGCAA
     3781 GTTGCCTCGC CCCCTTCCCA GAAGATCATG GGCATCTCGT CTTCTTCTAA TTTCGCATTT
     3841 TGTGAAGCTC CATTCATGCA CGTCGGCTTC TGGACGACTC TTATCATATG CATTGGCCTG
     3901 CTCGGTGTGA GGATGAGCCA CTCCCACATG TTTAGACGGC AGGGGCCTCT TGCCACCCGT
     3961 TGAATTTTTG GTGCGAAGAC GTGCCTGGGA ACGACCTCCA CTTTAAATTT CTCCGTTGTT
     4021 GCGGTCGCTC CATCCTCCTC ACATTTCTTG CCTCCTAAGT CCCATGTGCC ACACTCGGTG
     4081 AAAAACGGAG TTTGATACGG ATGAAATTTC CAGTGAGCTT TTCACATTCT GGAGGTTTCG
     4141 AAAAAAATTG TGGGATTTTC TGAATTAATC CGAGAATCCG CCCTGCCCAT CAACCGGAAA
     4201 GAATTAAATC CCTCGCACAT TCCTTTCTCC CACCACCTTC TTTATCTCTA CATTCTTTAA
     4261 CCAAAACACC TAGGTGAGTA CCCAAAGTCT TTTGACACCT TTGAAAATCC GCGCCAGTCG
     4321 TAGGGCACGT AAAACAATTC TCGCTGGTGG CTTCTGCAGG TGAAGTCGGG GCAGAGTGGA
     4381 GCTGAGCATG GTCGACCACC CACATCATTC TGTCTTGTTT TTGGCGATGT CTTCCATGCT
     4441 TTTGCCCTCA TCTGTTGCGT CGACTGCAGA GTACTGTTTT GCCACTACTG AGCTCAACAT
     4501 GGGCTTGTGT GTGGTGAAGA AAAAAATTTG GTGGCTCTTG AAAATTTTCA ACCCATCGGA
     4561 TACATGCCCT TCCAACTCAC AACGCCCTAA CACTTTATTG TCCTATCATT GCCTCTTCGC
     4621 ACATGCTAAC GATCTCCACA GAAAACTTCA AAATGCCTCA ATCCTGGGAA GAACTGGCCG
     4681 CTGATAAGCG CGCCCGCCTC GCAAAAACCA TCCCTGATGA ATGGAAAGTC CAGACGCTGC
     4741 CTGCGGAAGA CAGCGTTATT GATTTCCCAA AGAAATCGGG GATCCTTTCA GAGGCCGAAC
     4801 TGAAGATCAC AGAGGCCTCC GCTGCAGATC TTGTGTCCAA GCTGGCGGCC GGAGAGTTGA
     4861 CCTCGGTGGA AGTTACGCTA GCATTCTGTA AACGGGCAGC AATCGCCCAG CAGTTAACAA
     4921 ACTGCGCCCA CGAGTTCTTC CCTGACGCCG CTCTCGCGCA GGCAAGGGAA CTCGATGAAT
     4981 ACTACGCAAA GCACAAGAGA CCCGTTGGTC CACTCCATGG CCTCCCCATC TCTCTCAAAG
     5041 ACCAGCTTCG AGTCAAGGGC TACGAAACAT CAATGGGCTA CATCTCATGG CTAAACAAGT
     5101 ACGACGAAGG GGACTCGGTT CTGACAACCA TGCTCCGCAA AGCCGGTGCC GTCTTCTACG
     5161 TCAAGACCTC TGTCCCGCAG ACCCTGATGG TCTGCGAGAC AGTCAACAAC ATCATCGGGC
     5221 GCACCGTCAA CCCACGCAAC AAGAACTGGT CGTGCGGCGG CAGTTCTGGT GGTGAGGGTG
     5281 CGATCGTTGG GATTCGTGGT GGCGTCATCG GTGTAGGAAC GGATATCGGT GGCTCGATTC
     5341 GAGTGCCGGC CGCGTTCAAC TTCCTGTACG GTCTAAGGCC GAGTCATGGG CGGCTGCCGT
     5401 ATGCAAAGAT GGCGAACAGC ATGGAGGGTC AGGAGACGGT GCACAGCGTT GTCGGGCCGA
     5461 TTACGCACTC TGTTGAGGAC CTCCGCCTCT TCACCAAATC CGTCCTCGGT CAGGAGCCAT
     5521 GGAAATACGA CTCCAAGGTC ATCCCCATGC CCTGGCGCCA GTCCGAGTCG GACATTATTG
     5581 CCTCCAAGAT CAAGAACGGC GGGCTCAATA TCGGCTACTA CAACTTCGAC GGCAATGTCC
     5641 TTCCACACCC TCCTATCCTG CGCGGCGTGG AAACCACCGT CGCCGCACTC GCCAAAGCCG
     5701 GTCACACCGT GACCCCGTGG ACGCCATACA AGCACGATTT CGGCCACGAT CTCATCTCCC
     5761 ATATCTACGC GGCTGACGGC AGCGCCGACG TAATGCGCGA TATCAGTGCA TCCGGCGAGC
     5821 CGGCGATTCC AAATATCAAA GACCTACTGA ACCCGAACAT CAAAGCTGTT AACATGAACG
     5881 AGCTCTGGGA CACGCATCTC CAGAAGTGGA ATTACCAGAT GGAGTACCTT GAGAAATGGC
     5941 GGGAGGCTGA AGAAAAGGCC GGGAAGGAAC TGGACGCCAT CATCGCGCCG ATTACGCCTA
     6001 CCGCTGCGGT ACGGCATGAC CAGTTCCGGT ACTATGGGTA TGCCTCTGTG ATCAACCTGC
     6061 TGGATTTCAC GAGCGTGGTT GTTCCGGTTA CCTTTGCGGA TAAGAACATC GATAAGAAGA
     6121 ATGAGAGTTT CAAGGCGGTT AGTGAGCTTG ATGCCCTCGT GCAGGAAGAG TATGATCCGG
     6181 AGGCGTACCA TGGGGCACCG GTTGCAGTGC AGGTTATCGG ACGGAGACTC AGTGAAGAGA
     6241 GGACGTTGGC GATTGCAGAG GAAGTGGGGA AGTTGCTGGG AAATGTGGTG ACTCCATAGA
     6301 AGGTTGAGCG CGTAATAGTG GGTTGGTCTT GTCTCTATCC ATTCATCTGT GAGACATACT
     6361 GATTTCAACT TTTTCATCAG ATAATAAGGA TGGTGTGAGA TAAGCAAGGC TGGGTTATGA
     6421 GGTTTGGGAC TAGATGGTGT TTCGCGTCTA AAGGTGAGTC TTCGTTGGTT CAATCACATT
     6481 GATCTGATCG CTGATCAATT GTCAGCCTCT GAATGTGCTC TTGAAGAAAA TGGTTGTCTG
     6541 TAAGAATCCA GAACCAAAAA CGGTCATTTC AAGTATGTAT CCTCCGTGAA GTTCCTTTAG
     6601 CTCTTGCTAA TATTGGACTT TAGCGTGCTT TAGCCAAAGA CAGGGTCGCG AGGTTAAGAG
     6661 GATGGGCCAG GAAAAATCTA AGTTAGATTT GTCACACAAA GAAAAAGATT GTCTCAGTTT
     6721 TGGTTTTGTT TGGTTTGATT TATGCATAGG AAATATTTAT GATCGAAATC ACTCATTTCC
     6781 TGCTTCGTAA ATATCATCTA CTATATGCTT CGTAAATGTC CATCTCCTAC ATATTGTGGA
     6841 TATGATAAAT GCAATCCTCT CGTCTACACT CTCGGCTTAT GCCTTTGTCT TCTTTGCGCT
     6901 CCTTTTAGCC TTCTTTTTAT TCCTAGTCTT CAAGCCCATC TACGTATATG GAGAGGCTGT
     6961 CGAGCGGCGA GGGGCTTTTG AAGGGCGCCC TGGGGACATT AACTCAACAG GCCAGAGGTA
     7021 GAGTATACTT CGATGGAATC CTACGTTCCG GCGGTAAGTT ACTAAAGTTT GGTTGGTAAG
     7081 CTTGAAGCTG ATGTGGTCTT TTAGATTTCG AAATTGCGAA GAAAATGCTC AAAATGCTCC
     7141 AAGGTGATGG GGCAATTGAT GACGCAGCCG TCGAAGAGGT TGTATTGAAG GTCTCAAAAG
     7201 ACTTTTATGT ATGTGCGGAT AGCTGCAACA TTTACACTGG AAACATGAAG CTCGCATATG
     7261 ACGCGTAAGT CACTACTTAT CTTCTGTTAT TGATTATTTC TTACCCTGAT TTAGCTTATC
     7321 TGTTGCTCCT TCAACGCCGG CTACCGTCGC TGAGAGGCAA TACATCGAAG CTACGTCTCG
     7381 CCTATCGTCA TTCTCCACTT TCACTTTGTC GCCACGTGAA ATCAGACACG ATGTCAACCC
     7441 CTTAAGCCTA TTGTCAACGG TTTTATCTGA CAGTTCAAGC GATGCCTATA GACTTCCTGA
     7501 TTTGATGCTT GATTTGGCGG AAAAGCTGGG ATGTCGAAAT GAGATAGACC ACGGCCTCGT
     7561 TTGGGGGATG GTAGGACGCG CAGCGATTGC CAAGGAAGAT TGGACAATGG CAAGTAAGGC
     7621 TGTAGGAAAC ATGGTGCAAA CAGTCAAAAA GAGCCAGAGC AGCCAGACAG CTGTAAAACA
     7681 GGAGCAACAA ATAAATTTAA TTAACACAAG TATCGTGGCG CGCC
//
