LOCUS       pPEE34                  6083 bp    DNA     circular SYN 14-Mar-2019
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    1..750
                     /note="Safe Haven (CNAG_05670 side)"
     gene            complement(899..1756)
                     /note="bla"
     rep_origin      complement(1907..2574)
                     /note="pUC origin"
     gene            2916..3272
                     /note="lacZ"
     misc_feature    complement(2972..3079)
                     /note="MCS"
     CDS             3410..5305
                     /note="NEO Cassette"
     gene            4243..5037
                     /note="NEO (Tn5)"
     regulatory      5038..5305
                     /note="TRP1 terminator"
     misc_feature    5306..6055
                     /note="Safe Haven (CNAG_05671 side)"
     misc_difference 6056..6083
                     /note="Linker sequence"
BASE COUNT      1486 A      1614 C      1469 G      1514 T         0 OTHER
ORIGIN      ?
        1 CTGAAAACAT GCACTGTAAA ATGATAAATG CAATCGATGT TGTTTGTATA GGTAGAAAAA
       61 CAGGATCAAA AGGGGGGTAC ATCCTTTTCT GTGGATACCA GTACATGCAC ATCCATAGAC
      121 CATCTGCCAT TCTCTTTTGC TGTGATTCAT TGAAGTCCAG TGCCACCCCC AAATCAAGCT
      181 ATTCTTGCAC CGTCTATCCA TTTACATTTC CAGCCATGGT GAACACACCT TTCCAAGGTC
      241 TGTTACAATG GGAAGAGAAA CAAACAGGAA CCCATAGAAA GAAGGAAGGT GCCACAAGGG
      301 TCATGACAGG GTCCATTGCC ACACTTCAGC AGCAATTGGC AGCCCTATGC ATGAGAATAA
      361 AAATAATAAA ATATCATGCA TAGGACAAAG GCTGGACAGA GCCCAATGTC ACATTTCAGC
      421 ATCAGACTGG ACAAAGCCTG GTGCCATGTT TCAGCATCAA GCTAGACAAA GCCTAATGCT
      481 GCATTTCAGC ACCAATTTTC ACCTGCCAAC ATCATCCCAT GCATGGGAAT AAATCCTACC
      541 ATGTGGTGAG AACAAGTGGT GGTGAGGGCT CTGCACTTAC ACAGGAATAT CAGCAATCCC
      601 AGCGAACAGG TATGATACTT CTTCTGTTCC ACCCTATTTG TCATGAGCCT ACATGAAGTA
      661 GAAGAAATAA GAGATCATAG GAGGGAGTTA TGTAATGAAG GGAAGCAAGA TTGTTTCAGA
      721 TAAGGAGGTC CAGCTGGAGT ACTTTGTGAA GCCGCTACAG GGCGCGTCAG GTGGCACTTT
      781 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
      841 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT
      901 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT
      961 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG
     1021 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA
     1081 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG
     1141 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT
     1201 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG
     1261 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG
     1321 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA
     1381 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC
     1441 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC
     1501 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC
     1561 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG
     1621 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC
     1681 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC
     1741 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT
     1801 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC
     1861 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
     1921 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
     1981 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
     2041 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG
     2101 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
     2161 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
     2221 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
     2281 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
     2341 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
     2401 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
     2461 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
     2521 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
     2581 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
     2641 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
     2701 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
     2761 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
     2821 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
     2881 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC
     2941 AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG
     3001 AACTAGTGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTATCGATA CCGTCGACCT
     3061 CGAGGGGGGG CCCGGTACCC AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG
     3121 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG
     3181 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT
     3241 CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGAAATTGTA AGCGTTAATA TTTTGTTAAA
     3301 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTTAA CCAATAGGCC GAAATCGGCA
     3361 AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTCCATGG CTGCGAGGAT
     3421 GTGAGCTGGA GAGCGGCGCA CGGGAAGGGG AGGACTCACA TAAGCATGCA GGATTCGAGT
     3481 GGCATGGTGT GCACTGAGTG TATGGTTGTC GGAGGAGAGG ATGATGGTAA CAACAATAGC
     3541 AGCAACGTCA CTCGACGCGC GTCCGGTGTG CCACACGGGG TAACGCCGAG TCGCCGTCAG
     3601 GGTCGCCGAG ACCACTCTCA CAGCGTCACC GTTGGCACCA GCTCAGCTTA CAGCTTCTAT
     3661 CCTCCGCCAG CATCCACATA CATCCCCTAT ACCGCATCCC CCACCCACTG CCCAAGGTGA
     3721 GTCATCTTCC CGCCCCCTTC CCTTGCCCGC CACTCAGTCC TCCATCCTCC ACTAATCCAC
     3781 CTTATCGCAC CCACCGCCTA TCGCACATCC GAGCACAATG CTGGGCCTGC CAGGGGCTGC
     3841 TAGATGGTGC TCTCCCCACG CTGATCTGCA TGCCGGCCAT TGGATCATGG GTGCTAGGTG
     3901 CTGGGTGCTG GATGTTGGAT GCTGGATGCT GGGTGCACGC TTGGTCATTT CCTTCCAGGA
     3961 TTGACGGTCG CCGAGAGGAC GACGTGGCGT TCGACAACGA GGGCCGATAG CACCGCATCG
     4021 CCTCGACCTG CATCCATCTC GCCTTGTCCT TTTGGTGCAA CAATCCATCC GTGCTGGTGC
     4081 CACACGCATA GCTGGAAGAG ATGGATGTGC GTTGAACAGA GCTGCCGTCA GGACTTTTTG
     4141 GTGCACGGAC CCTATTGTCC TCCCCAATCT TCACCGCGTC TCCTAATATG CAGCCTCTTT
     4201 GCTAATTGTC TTTTTCCATT AGTAAACTCG CCCAACATGT CTATGATTGA ACAAGATGGA
     4261 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
     4321 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
     4381 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG
     4441 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
     4501 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCCCAC
     4561 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
     4621 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
     4681 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
     4741 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG
     4801 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
     4861 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT
     4921 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC
     4981 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGTG
     5041 AAGGCGGTAA GGGGTTAATT TTCCTTAGAG GGTGTATATA TACATATTAG AGAAGTGATA
     5101 CAATTTTAGC ACACTGCGAA TTCGAGACAG ACATCGTGTC AATCATCTTT TTTGACATTA
     5161 TATGCCCATT AATCTATCTA CAGACAACAA TACCATCCTT CCCACCCTCA GCAACGCCGT
     5221 TGAATCCTCA GGATCTTCAT GGCTCCTTGT CTCTGAAACC AGGAAGCTAG TTTCTACATC
     5281 TCTTCCGTGT TAATACAGAT AAACCGGTCC TCAAGCTCCC AGTACTCACT CTCATCTTAC
     5341 CTTGCACCAC CTTCAACATA AACTTACTCC ACTAGTATAT AAGCTTGCCT GATCTTCCCA
     5401 AGTGAATATA CCTCTGTCCT ATACCATATG CCAACAGACA AGTGTCAAGA GGTATATCAT
     5461 ACATATATGT CATGATGCTA TGCAGAAGTG ACACCCGAAA CCTCCACCTC TCTCAGCTGC
     5521 CGAACCAAAG TACCTCCACC TCTGATGACT GCAGCTGATC GTCTAAATTC TCTTTGGGTT
     5581 CTCGACATCT CTCTTCACCC TTCGGCCTCT TTCCAGCCAT CTCTCTGCCT TCCTGTCTCC
     5641 CTCGAGCTAT CTATACATGC TCTGGTCAGC ATGCTCACCA CCTCCTCGGG CCCCATCCGG
     5701 TACCTGAGTC AGCAGTATGA GAAAGATGAG TAAGCAAGGA AAACTAGGAA AAGTATATAA
     5761 ACCATAGTTT ATAGTTTTCA TAGCTTTCCT CAGAAAATCA ATACTGTTGT CTTCTCTTAC
     5821 CTTGTCCCAG TCCCTTTGCC TCTTTCCACC TTCCAGACCT TCCAACATCT AAAAGTCGCA
     5881 TCGGTCAGCC ATTAGTCTGG CTCCTGTTCC CTTTCAGGCC TCGGCTATTC TGTCCTCATC
     5941 CTTCCTTCTC GCCACCCTCT TCGATTGAGC GTCGGGCTTT TACGCTGACC GGCGTGACAT
     6001 CCCTGACAAT ATATCTCATT GCTTAGTTAA ACTACAGCTT TCTATCTCGT CTCCATTAAT
     6061 TAACACAAGT ATCGTGGCGC GCC
//
