LOCUS       pJL1_PorA_4xDQNA        2995 bp ds-DNA     circular     04-JUN-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    716..1834
                     /locus_tag="PorA"
                     /label="PorA"
                     /ApEinfo_label="PorA"
                     /ApEinfo_fwdcolor="#6666ff"
                     /ApEinfo_revcolor="#6666ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1..620
                     /vntifkey="33"
                     /locus_tag="pBR322 origin"
                     /label="pBR322 origin"
                     /ApEinfo_label="pBR322 origin"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    698..704
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        633..651
                     /vntifkey="30"
                     /note="Geneious type: Promoter"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1835..1918
                     /locus_tag="4xDQNAT"
                     /label="4xDQNAT"
                     /ApEinfo_label="4xDQNAT"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1883..1897
                     /locus_tag="DQNAT"
                     /label="DQNAT"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1904..1918
                     /locus_tag="DQNAT(1)"
                     /label="DQNAT(1)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1925..1942
                     /locus_tag="6xHis"
                     /label="6xHis"
                     /ApEinfo_label="6xHis"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2016..2059
                     /vntifkey="43"
                     /note="Geneious type: Terminator"
                     /locus_tag="T7 terminator"
                     /label="T7 terminator"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1946..1951
                     /locus_tag="SalI"
                     /label="SalI"
                     /ApEinfo_label="SalI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1841..1855
                     /locus_tag="DQNAT(2)"
                     /label="DQNAT(2)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1862..1876
                     /locus_tag="DQNAT(3)"
                     /label="DQNAT(3)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    710..715
                     /locus_tag="NdeI"
                     /label="NdeI"
                     /ApEinfo_label="NdeI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2076..2890)
                     /vntifkey="21"
                     /note="Geneious type: Marker"
                     /locus_tag="kanR resistance gene"
                     /label="kanR resistance gene"
                     /ApEinfo_label="kanR resistance gene"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
       61 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      121 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      181 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      241 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      301 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      361 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      421 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      481 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      541 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      601 GGAAAAACGC CAGCAACGCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGAGACCACA
      661 ACGGTTTCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGgacgt
      721 cagtttgtat gGAGAGATTA AGGCAGGGGT TGAGGGGCGC AATTACCAAC TTCAACTTAC
      781 GGAAGCGCAG GCAGCTAATG GTGGGGCGAG TGGCCAGGTC AAAGTCACGA AGGTTACTAA
      841 GGCAAAGTCC CGCATTCGTA CGAAGATTTC CGACTTTGGC TCATTTATTG GTTTCAAGGG
      901 TTCCGAGGAC TTAGGCGATG GCTTGAAAGC AGTTTGGCAG CTTGAGCAAG ATGTGAGTGT
      961 GGCGGGAGGG GGCGCTACCC AGTGGGGTAA CCGTGAGTCT TTTATCGGGT TAGCAGGCGA
     1021 GTTCGGAACT TTACGTGCCG GGCGCGTAGC AAACCAGTTT GACGATGCCT CCCAGGCCAT
     1081 CGACCCCTGG GATTCTAATA ATGACGTAGC AAGCCAACTG GGAATTTTTA AGCGCCACGA
     1141 TGATATGCCT GTCTCCGTTC GTTACGACAG TCCGGAGTTT AGCGGGTTCT CGGGATCTGT
     1201 GCAATTCGTT CCGATCCAAA ACAGTAAATC TGCCTATACA CCGGCATACT ATACAAAAAA
     1261 CACAAATAAT AACTTGACCT TAGTACCAGC CGTGGTTGGA AAGCCAGGTT CTGATGTATA
     1321 TTATGCGGGA CTGAACTACA AAAACGGAGG GTTCGCCGGT AACTATGCGT TTAAGTACGC
     1381 CCGCCACGCT AACGTCGGCC GTAACGCCTT TGAACTGTTC TTGATTGGGA GCGGATCGGA
     1441 TCAAGCGAAA GGAACAGACC CCCTGAAAAA CCATCAAGTC CACCGTTTGA CCGGAGGATA
     1501 TGAGGAGGGG GGTTTGAACT TGGCATTAGC CGCGCAGTTG GACTTATCGG AAAACGGCGA
     1561 CAAAACTAAG AATTCCACTA CTGAAATCGC AGCTACTGCG AGCTATCGTT TTGGGAATGC
     1621 AGTGCCTCGT ATTTCATACG CTCACGGCTT CGATTTCATT GAGCGCGGCA AAAAGGGAGA
     1681 AAATACGTCC TACGACCAAA TTATCGCCGG TGTAGATTAT GACTTCTCTA AGCGTACTAG
     1741 CGCAATCGTG AGCGGCGCTT GGTTGAAGCG CAATACGGGT ATCGGGAATT ATACCCAAAT
     1801 CAATGCCGCA TCTGTCGGCT TGCGTCACAA ATTTCTCGAG GATCAGAACG CGACCGGCGG
     1861 TGACCAAAAT GCCACAGGTG GCGATCAAAA CGCCACCGGC GGTGACCAGA ATGCGACAGT
     1921 CGACCATCAC CATCATCACC ATTAAGTCGA CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT
     1981 GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     2041 GTCTTGAGGG GTTTTTTGCT GAAAGCCAAT TCTGATTAGA AAAACTCATC GGCATCAAAT
     2101 GAAACTGCAA TTTATTCATA TCAGGATTAT CAATACCATA TTTTTGAAAA AGCCGTTTCT
     2161 GTAATGAAGG AGAAAACTCA CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT
     2221 CTGCGATTCC GACTCGTCCA ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA
     2281 GGTTATCAAG TGAGAAATCA CCATGAGTGA CGACTGAATC CGGTGAGAAT GGCAAAAGCT
     2341 TATGCATTTC TTTCCAGACT TGTTCAACAG GCCAGCCATT ACGCTCGTCA TCAAAATCAC
     2401 TCGCATCAAC CAAACCGTTA TTCATTCGTG ATTGCGCCTG AGCGAGACGA AATACGCGAT
     2461 CGCTGTTAAA AGGACAATTA CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA
     2521 GCGCATCAAC AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT
     2581 TCCCGGGGAT CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA AAATGCTTGA
     2641 TGGTCGGAAG AGGCATAAAT TCCGTCAGCC AGTTTAGTCT GACCATCTCA TCTGTAACAT
     2701 CATTGGCAAC GCTACCTTTG CCATGTTTCA GAAACAACTC TGGCGCATCG GGCTTCCCAT
     2761 ACAATCGATA GATTGTCGCA CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT
     2821 ATAAATCAGC ATCCATGTTG GAATTTAATC GCGGCTTCGA GCAAGACGTT TCCCGTTGAA
     2881 TATGGCTCAT AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT ATTGTTCATG
     2941 ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT TTGAGACACA ACGTG
//
