LOCUS       pJL1_TTc_4xDQNAT        3229 bp ds-DNA     circular     04-JUN-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    716..2068
                     /locus_tag="C. tetani toxin fragment C domain"
                     /label="C. tetani toxin fragment C domain"
                     /ApEinfo_label="C. tetani toxin fragment C domain"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1..620
                     /vntifkey="33"
                     /locus_tag="pBR322 origin"
                     /label="pBR322 origin"
                     /ApEinfo_label="pBR322 origin"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    698..704
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        633..651
                     /vntifkey="30"
                     /note="Geneious type: Promoter"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    710..715
                     /locus_tag="NdeI"
                     /label="NdeI"
                     /ApEinfo_label="NdeI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2069..2152
                     /locus_tag="4xDQNAT"
                     /label="4xDQNAT"
                     /ApEinfo_label="4xDQNAT"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2117..2131
                     /locus_tag="DQNAT"
                     /label="DQNAT"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2138..2152
                     /locus_tag="DQNAT(1)"
                     /label="DQNAT(1)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2159..2176
                     /locus_tag="6xHis"
                     /label="6xHis"
                     /ApEinfo_label="6xHis"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2250..2293
                     /vntifkey="43"
                     /note="Geneious type: Terminator"
                     /locus_tag="T7 terminator"
                     /label="T7 terminator"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2180..2185
                     /locus_tag="SalI"
                     /label="SalI"
                     /ApEinfo_label="SalI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2075..2089
                     /locus_tag="DQNAT(2)"
                     /label="DQNAT(2)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2096..2110
                     /locus_tag="DQNAT(3)"
                     /label="DQNAT(3)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2310..3124)
                     /vntifkey="21"
                     /note="Geneious type: Marker"
                     /locus_tag="kanR resistance gene"
                     /label="kanR resistance gene"
                     /ApEinfo_label="kanR resistance gene"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
       61 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      121 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      181 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      241 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      301 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      361 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      421 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      481 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      541 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      601 GGAAAAACGC CAGCAACGCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGAGACCACA
      661 ACGGTTTCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGAAGAA
      721 TCTGGATTGC TGGGTGGACA ATGAAGAAGA CATCGACGTG ATTCTTAAAA AGAGTACGAT
      781 CCTTAACTTG GATATTAACA ATGACATTAT TTCAGACATC TCTGGTTTCA ATAGTTCAGT
      841 CATTACATAT CCGGATGCCC AACTGGTTCC AGGAATCAAT GGAAAGGCGA TCCACCTTGT
      901 GAACAATGAG TCTAGCGAGG TGATTGTCCA TAAGGCTATG GACATCGAAT ACAACGATAT
      961 GTTTAACAAC TTCACAGTAT CGTTTTGGCT GCGTGTACCA AAAGTCTCAG CTTCTCATTT
     1021 GGAACAATAC GATACAAATG AATACTCCAT CATCAGCTCT ATGAAGAAGT ATAGCCTTTC
     1081 GATCGGCAGT GGTTGGTCCG TGTCCTTAAA AGGTAATAAC TTAATCTGGA CATTAAAAGA
     1141 TTCAGCCGGC GAAGTCCGTC AGATCACTTT CCGCGACCTT TCCGATAAAT TTAACGCTTA
     1201 CTTAGCAAAC AAATGGGTAT TCATTACCAT CACAAACGAC CGTTTGAGCT CTGCCAATTT
     1261 GTACATTAAC GGTGTTTTAA TGGGTAGCGC AGAGATCACG GGCCTTGGGG CTATCCGTGA
     1321 GGACAATAAT ATCACACTTA AGCTGGATCG CTGCAATAAC AACAATCAAT ATGTGAGCAT
     1381 CGACAAATTT CGCATTTTTT GTAAGGCTCT TAACCCCAAG GAGATCGAAA AGTTATATAC
     1441 GAGTTACCTG AGCATTACCT TTCTGCGCGA CTTTTGGGGG AATCCGTTAC GCTATGACAC
     1501 AGAATATTAT TTAATCCCTG TGGCATACTC TTCGAAAGAC GTCCAGCTGA AAAACATTAC
     1561 CGACTATATG TACTTAACGA ACGCTCCAAG CTACACGAAT GGAAAATTAA ACATCTATTA
     1621 CCGTCGTTTA TATTCTGGTC TGAAATTTAT CATTAAACGC TATACACCCA ATAATGAGAT
     1681 TGATTCGTTC GTCCGTTCCG GTGACTTCAT TAAGTTGTAT GTTTCGTACA ACAACAACGA
     1741 ACATATTGTT GGGTACCCCA AAGATGGAAA CGCTTTCAAT AATTTGGACC GCATCTTACG
     1801 TGTAGGCTAC AACGCTCCAG GGATCCCTCT TTACAAAAAA ATGGAAGCGG TAAAACTTCG
     1861 CGACTTAAAA ACTTATTCAG TCCAGCTTAA GCTTTATGAC GATAAAGATG CCTCGCTTGG
     1921 CCTTGTTGGG ACTCATAACG GCCAAATCGG TAATGACCCC AACCGCGACA TCCTTATTGC
     1981 ATCCAATTGG TACTTTAATC ACCTGAAGGA TAAGACTCTG ACTTGCGATT GGTACTTCGT
     2041 ACCGACTGAC GAAGGGTGGA CGAACGATCT CGAGGATCAG AACGCGACCG GCGGTGACCA
     2101 AAATGCCACA GGTGGCGATC AAAACGCCAC CGGCGGTGAC CAGAATGCGA CAGTCGACCA
     2161 TCACCATCAT CACCATTAAG TCGACCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG
     2221 GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     2281 AGGGGTTTTT TGCTGAAAGC CAATTCTGAT TAGAAAAACT CATCGGCATC AAATGAAACT
     2341 GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG
     2401 AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA
     2461 TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT
     2521 CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGCTTATGCA
     2581 TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT
     2641 CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT
     2701 TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT
     2761 CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCGG
     2821 GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG
     2881 GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG
     2941 CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC
     3001 GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT
     3061 CAGCATCCAT GTTGGAATTT AATCGCGGCT TCGAGCAAGA CGTTTCCCGT TGAATATGGC
     3121 TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGATGATA
     3181 TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA CACAACGTG 
//
