LOCUS       pJL1_CRM197_4xDQ        3481 bp ds-DNA     circular     04-JUN-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    716..2320
                     /locus_tag="C. diphtheriae toxin"
                     /label="C. diphtheriae toxin"
                     /ApEinfo_label="C. diphtheriae toxin"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    869..871
                     /locus_tag="G52E mutation"
                     /label="G52E mutation"
                     /ApEinfo_label="G52E mutation"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1..620
                     /vntifkey="33"
                     /locus_tag="pBR322 origin"
                     /label="pBR322 origin"
                     /ApEinfo_label="pBR322 origin"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    698..704
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        633..651
                     /vntifkey="30"
                     /note="Geneious type: Promoter"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    710..715
                     /locus_tag="NdeI"
                     /label="NdeI"
                     /ApEinfo_label="NdeI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2321..2404
                     /locus_tag="4xDQNAT"
                     /label="4xDQNAT"
                     /ApEinfo_label="4xDQNAT"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2369..2383
                     /locus_tag="DQNAT"
                     /label="DQNAT"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2390..2404
                     /locus_tag="DQNAT(1)"
                     /label="DQNAT(1)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2411..2428
                     /locus_tag="6xHis"
                     /label="6xHis"
                     /ApEinfo_label="6xHis"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2502..2545
                     /vntifkey="43"
                     /note="Geneious type: Terminator"
                     /locus_tag="T7 terminator"
                     /label="T7 terminator"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2432..2437
                     /locus_tag="SalI"
                     /label="SalI"
                     /ApEinfo_label="SalI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2327..2341
                     /locus_tag="DQNAT(2)"
                     /label="DQNAT(2)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2348..2362
                     /locus_tag="DQNAT(3)"
                     /label="DQNAT(3)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2562..3376)
                     /vntifkey="21"
                     /note="Geneious type: Marker"
                     /locus_tag="kanR resistance gene"
                     /label="kanR resistance gene"
                     /ApEinfo_label="kanR resistance gene"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
       61 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      121 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      181 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      241 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      301 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      361 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      421 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      481 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      541 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      601 GGAAAAACGC CAGCAACGCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGAGACCACA
      661 ACGGTTTCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGGCGC
      721 TGATGATGTT GTTGATTCTT CTAAATCTTT TGTGATGGAA AACTTTTCTT CGTACCACGG
      781 GACTAAACCT GGTTATGTAG ATTCCATTCA AAAAGGTATA CAAAAGCCAA AATCTGGTAC
      841 ACAAGGAAAT TATGACGATG ATTGGAAAGA ATTTTATAGT ACCGACAATA AATACGACGC
      901 TGCGGGATAC TCTGTAGATA ATGAAAACCC GCTCTCTGGA AAAGCTGGAG GCGTGGTCAA
      961 AGTGACGTAT CCAGGACTGA CGAAGGTTCT CGCACTAAAA GTGGATAATG CCGAAACTAT
     1021 TAAGAAAGAG TTAGGTTTAA GTCTCACTGA ACCGTTGATG GAGCAAGTCG GAACGGAAGA
     1081 GTTTATCAAA AGGTTCGGTG ATGGTGCTTC GCGTGTAGTG CTCAGCCTTC CCTTCGCTGA
     1141 GGGGAGTTCT AGCGTTGAAT ATATTAATAA CTGGGAACAG GCGAAAGCGT TAAGCGTAGA
     1201 ACTTGAGATT AATTTTGAAA CCCGTGGAAA ACGTGGCCAA GATGCGATGT ATGAGTATAT
     1261 GGCTCAAGCC TGTGCAGGAA ATCGTGTCAG GCGATCAGTA GGTAGCTCAT TGTCATGCAT
     1321 AAATCTTGAT TGGGATGTCA TAAGGGATAA AACTAAGACA AAGATAGAGT CTTTGAAAGA
     1381 GCATGGCCCT ATCAAAAATA AAATGAGCGA AAGTCCCAAT AAAACAGTAT CTGAGGAAAA
     1441 AGCTAAACAA TACCTAGAAG AATTTCATCA AACGGCATTA GAGCATCCTG AATTGTCAGA
     1501 ACTTAAAACC GTTACTGGGA CCAATCCTGT ATTCGCTGGG GCTAACTATG CGGCGTGGGC
     1561 AGTAAACGTT GCGCAAGTTA TCGATAGCGA AACAGCTGAT AATTTGGAAA AGACAACTGC
     1621 TGCTCTTTCG ATACTTCCTG GTATCGGTAG CGTAATGGGC ATTGCAGACG GTGCCGTTCA
     1681 CCACAATACA GAAGAGATAG TGGCACAATC AATAGCTTTA TCATCTTTAA TGGTTGCTCA
     1741 AGCTATTCCA TTGGTAGGAG AGCTAGTTGA TATTGGTTTC GCTGCATATA ATTTTGTAGA
     1801 GAGTATTATC AATTTATTTC AAGTAGTTCA TAATTCGTAT AATCGTCCCG CGTATTCTCC
     1861 GGGGCATAAA ACGCAACCAT TTCTTCATGA CGGGTATGCT GTCAGTTGGA ACACTGTTGA
     1921 AGATTCGATA ATCCGAACTG GTTTTCAAGG GGAGAGTGGG CACGACATAA AAATTACTGC
     1981 TGAAAATACC CCGCTTCCAA TCGCGGGTGT CCTACTACCG ACTATTCCTG GAAAGCTGGA
     2041 CGTTAATAAG TCCAAGACTC ATATTTCCGT AAATGGTCGG AAAATAAGGA TGCGTTGCAG
     2101 AGCTATAGAC GGTGATGTAA CTTTTTGTCG CCCTAAATCT CCTGTTTATG TTGGTAATGG
     2161 TGTGCATGCG AATCTTCACG TGGCATTTCA CAGAAGCAGC TCGGAGAAAA TTCATTCTAA
     2221 TGAAATTTCA TCGGATTCCA TAGGCGTTCT TGGGTACCAG AAAACAGTAG ATCACACCAA
     2281 GGTTAATTCT AAGCTATCGC TATTTTTTGA AATCAAAAGC CTCGAGGATC AGAACGCGAC
     2341 CGGCGGTGAC CAAAATGCCA CAGGTGGCGA TCAAAACGCC ACCGGCGGTG ACCAGAATGC
     2401 GACAGTCGAC CATCACCATC ATCACCATTA AGTCGACCGG CTGCTAACAA AGCCCGAAAG
     2461 GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     2521 AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GCCAATTCTG ATTAGAAAAA CTCATCGGCA
     2581 TCAAATGAAA CTGCAATTTA TTCATATCAG GATTATCAAT ACCATATTTT TGAAAAAGCC
     2641 GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA TAGGATGGCA AGATCCTGGT
     2701 ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC TATTAATTTC CCCTCGTCAA
     2761 AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC TGAATCCGGT GAGAATGGCA
     2821 AAAGCTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA GCCATTACGC TCGTCATCAA
     2881 AATCACTCGC ATCAACCAAA CCGTTATTCA TTCGTGATTG CGCCTGAGCG AGACGAAATA
     2941 CGCGATCGCT GTTAAAAGGA CAATTACAAA CAGGAATCGA ATGCAACCGG CGCAGGAACA
     3001 CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA TTCTTCTAAT ACCTGGAATG
     3061 CTGTTTTCCC GGGGATCGCA GTGGTGAGTA ACCATGCATC ATCAGGAGTA CGGATAAAAT
     3121 GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT TAGTCTGACC ATCTCATCTG
     3181 TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA CAACTCTGGC GCATCGGGCT
     3241 TCCCATACAA TCGATAGATT GTCGCACCTG ATTGCCCGAC ATTATCGCGA GCCCATTTAT
     3301 ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG CTTCGAGCAA GACGTTTCCC
     3361 GTTGAATATG GCTCATAACA CCCCTTGTAT TACTGTTTAT GTAAGCAGAC AGTTTTATTG
     3421 TTCATGATGA TATATTTTTA TCTTGTGCAA TGTAACATCA GAGATTTTGA GACACAACGT
     3481 G     
//
