LOCUS       pJL1_EPA_DNNNS_D        3664 bp ds-DNA     circular     04-JUN-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2594..2611
                     /locus_tag="6x His"
                     /label="6x His"
                     /ApEinfo_label="6x His"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1..620
                     /vntifkey="33"
                     /locus_tag="pBR322 origin"
                     /label="pBR322 origin"
                     /ApEinfo_label="pBR322 origin"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    698..704
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        633..651
                     /vntifkey="30"
                     /note="Geneious type: Promoter"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    710..715
                     /locus_tag="NdeI"
                     /label="NdeI"
                     /ApEinfo_label="NdeI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    716..2587
                     /locus_tag="P. aeruginosa exotoxin A"
                     /label="P. aeruginosa exotoxin A"
                     /ApEinfo_label="P. aeruginosa exotoxin A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2745..3559)
                     /vntifkey="21"
                     /note="Geneious type: Marker"
                     /locus_tag="kanR resistance gene"
                     /label="kanR resistance gene"
                     /ApEinfo_label="kanR resistance gene"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1862..1876
                     /locus_tag="DQNRT"
                     /label="DQNRT"
                     /ApEinfo_label="DQNRT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1436..1453
                     /locus_tag="DNNNS"
                     /label="DNNNS"
                     /ApEinfo_label="DNNNS"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2685..2728
                     /vntifkey="43"
                     /note="Geneious type: Terminator"
                     /locus_tag="T7 terminator"
                     /label="T7 terminator"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2615..2620
                     /locus_tag="SalI"
                     /label="SalI"
                     /ApEinfo_label="SalI"
                     /ApEinfo_fwdcolor="#ffd478"
                     /ApEinfo_revcolor="#ffd478"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
       61 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      121 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      181 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      241 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      301 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      361 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      421 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      481 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      541 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      601 GGAAAAACGC CAGCAACGCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGAGACCACA
      661 ACGGTTTCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCCGA
      721 GGAAGCCTTC GACCTCTGGA ACGAATGCGC CAAGGCCTGC GTGCTCGACC TCAAGGACGG
      781 CGTGCGTTCC AGCCGCATGA GCGTCGACCC GGCCATCGCC GACACCAACG GCCAGGGCGT
      841 GCTGCACTAC TCCATGGTCC TGGAGGGCGG CAACGACGCG CTCAAGCTGG CCATCGACAA
      901 CGCCCTCAGC ATCACCAGCG ACGGCCTGAC CATCCGCCTC GAAGGCGGCG TCGAGCCGAA
      961 CAAGCCGGTG CGCTACAGCT ACACGCGCCA GGCGCGCGGC AGTTGGTCGC TGAACTGGCT
     1021 GGTACCGATC GGCCACGAGA AGCCCTCGAA CATCAAGGTG TTCATCCACG AACTGAACGC
     1081 CGGTAACCAG CTCAGCCACA TGTCGCCGAT CTACACCATC GAGATGGGCG ACGAGTTGCT
     1141 GGCGAAGCTG GCGCGCGATG CCACCTTCTT CGTCAGGGCG CACGAGAGCA ACGAGATGCA
     1201 GCCGACGCTC GCCATCAGCC ATGCCGGGGT CAGCGTGGTC ATGGCTCAGG CCCAGCCGCG
     1261 CCGGGAAAAG CGCTGGAGCG AATGGGCCAG CGGCAAGGTG TTGTGCCTGC TCGACCCGCT
     1321 GGACGGGGTC TACAACTACC TCGCCCAGCA GCGCTGCAAC CTCGACGATA CCTGGGAAGG
     1381 CAAGATCTAC CGGGTGCTCG CCGGCAACCC GGCGAAGCAT GACCTGGACA TCAAGGATAA
     1441 TAATAATTCT ACTCCCACGG TCATCAGTCA TCGCCTGCAT TTCCCCGAGG GCGGCAGCCT
     1501 GGCCGCGCTG ACCGCGCACC AGGCCTGCCA CCTGCCGCTG GAGGCCTTCA CTCGTCATCG
     1561 CCAGCCGCGC GGCTGGGAAC AACTGGAGCA GTGCGGCTAT CCGGTGCAGC GGCTGGTCGC
     1621 CCTCTACCTG GCGGCGCGAC TGTCGTGGAA CCAGGTCGAC CAGGTGATCC GCAACGCCCT
     1681 GGCCAGCCCC GGCAGCGGCG GCGACCTGGG CGAAGCGATC CGCGAGCAGC CGGAGCAGGC
     1741 CCGTCTGGCC CTGACCCTGG CCGCCGCCGA GAGCGAGCGC TTCGTCCGGC AGGGCACCGG
     1801 CAACGACGAG GCCGGCGCGG CCAGCGCCGA CGTGGTGAGC CTGACCTGCC CCGTCGCCAA
     1861 AGATCAAAAT AGAACTAAAG GGGAATGCGC GGGCCCGGCG GACAGCGGCG ACGCCCTGCT
     1921 GGAGCGCAAC TATCCCACTG GCGCGGAGTT CCTCGGCGAC GGCGGCGACG TCAGCTTCAG
     1981 CACCCGCGGC ACGCAGAACT GGACGGTGGA GCGGCTGCTC CAGGCGCACC GCCAACTGGA
     2041 GGAGCGCGGC TATGTGTTCG TCGGCTACCA CGGCACCTTC CTCGAAGCGG CGCAAAGCAT
     2101 CGTCTTCGGC GGGGTGCGCG CGCGCAGCCA GGACCTCGAC GCGATCTGGC GCGGTTTCTA
     2161 TATCGCCGGC GATCCGGCGC TGGCCTACGG CTACGCCCAG GACCAGGAAC CCGACGCGCG
     2221 CGGCCGGATC CGCAACGGTG CCCTGCTGCG GGTCTATGTG CCGCGCTGGA GTCTGCCGGG
     2281 CTTCTACCGC ACCGGCCTGA CCCTGGCCGC GCCGGAGGCG GCGGGCGAGG TCGAACGGCT
     2341 GATCGGCCAT CCGCTGCCGC TGCGCCTGGA CGCCATCACC GGCCCCGAGG AGGAAGGCGG
     2401 GCGCGTGACC ATTCTCGGCT GGCCGCTGGC CGAGCGCACC GTGGTGATTC CCTCGGCGAT
     2461 CCCCACCGAC CCGCGCAACG TCGGCGGCGA CCTCGACCCG TCCAGCATCC CCGACAAGGA
     2521 ACAGGCGATC AGCGCCCTGC CGGACTACGC CAGCCAGCCC GGCAAACCGC CGCGCGAGGA
     2581 CCTGAAGGGC GGTCATCACC ATCATCACCA TTAAGTCGAC CGGCTGCTAA CAAAGCCCGA
     2641 AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     2701 TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGCCAATT CTGATTAGAA AAACTCATCG
     2761 GCATCAAATG AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA
     2821 GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT
     2881 GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT
     2941 CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC GGTGAGAATG
     3001 GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT
     3061 CAAAATCACT CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA
     3121 ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA
     3181 ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA
     3241 ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA GTACGGATAA
     3301 AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT
     3361 CTGTAACATC ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG
     3421 GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT
     3481 TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCTTCGAG CAAGACGTTT
     3541 CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA
     3601 TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA
     3661 CGTG     
//
