LOCUS       pTrc99s_MBP_4xDQ        5763 bp ds-DNA     circular     12-JUL-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2589..2651
                     /locus_tag="DsbA signal sequence"
                     /label="DsbA signal sequence"
                     /ApEinfo_label="DsbA signal sequence"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3825..3839
                     /locus_tag="DQNAT"
                     /label="DQNAT"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#fc8008"
                     /ApEinfo_revcolor="#fc8008"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2667..3776
                     /locus_tag="E. coli MBP"
                     /label="E. coli MBP"
                     /ApEinfo_label="E. coli MBP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2652..2666
                     /locus_tag="SfiI GGCCNNNN^NGGCC"
                     /label="SfiI GGCCNNNN^NGGCC"
                     /ApEinfo_label="SfiI GGCCNNNN^NGGCC"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2509..2582
                     /locus_tag="trc promoter"
                     /label="trc promoter"
                     /ApEinfo_label="trc promoter"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1..620
                     /vntifkey="33"
                     /locus_tag="pBR322 origin"
                     /label="pBR322 origin"
                     /ApEinfo_label="pBR322 origin"
                     /ApEinfo_fwdcolor="#fefe0a"
                     /ApEinfo_revcolor="#fefe0a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1186..2277
                     /locus_tag="LacI"
                     /label="LacI"
                     /ApEinfo_label="LacI"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3867..3884
                     /locus_tag="6xHis"
                     /label="6xHis"
                     /ApEinfo_label="6xHis"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4447..5568
                     /locus_tag="SpecR region"
                     /label="SpecR region"
                     /ApEinfo_label="SpecR region"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      3976..4133
                     /locus_tag="rrnB terminator"
                     /label="rrnB terminator"
                     /ApEinfo_label="rrnB terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3777..3860
                     /locus_tag="4xDQNAT"
                     /label="4xDQNAT"
                     /ApEinfo_label="4xDQNAT"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3783..3797
                     /locus_tag="DQNAT(1)"
                     /label="DQNAT(1)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4099..4142
                     /locus_tag="rrnB T1 terminator"
                     /label="rrnB T1 terminator"
                     /ApEinfo_label="rrnB T1 terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3804..3818
                     /locus_tag="DQNAT(2)"
                     /label="DQNAT(2)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4274..4301
                     /locus_tag="rrnB T2 terminator"
                     /label="rrnB T2 terminator"
                     /ApEinfo_label="rrnB T2 terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3846..3860
                     /locus_tag="DQNAT(3)"
                     /label="DQNAT(3)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4343..4371
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
       61 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      121 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
      181 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      241 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      301 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      361 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      421 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      481 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      541 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      601 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
      661 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
      721 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
      781 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
      841 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC
      901 GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC
      961 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG
     1021 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCAGATCAA
     1081 TTCGCGCGCG AAGGCGAAGC GGCATGCATT TACGTTGACA CCATCGAATG GTGCAAAACC
     1141 TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA
     1201 CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC
     1261 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG
     1321 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG
     1381 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG
     1441 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC
     1501 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG
     1561 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT
     1621 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC
     1681 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC
     1741 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT
     1801 AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC
     1861 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG
     1921 CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG
     1981 CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT
     2041 ATCCCGCCGT CAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC
     2101 CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA
     2161 CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
     2221 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
     2281 CAACGCAATT AATGTGAGTT AGCGCGAATT GATCTGGTTT GACAGCTTAT CATCGACTGC
     2341 ACGGTGCACC AATGCTTCTG GCGTCAGGCA GCCATCGGAA GCTGTGGTAT GGCTGTGCAG
     2401 GTCGTAAATC ACTGCATAAT TCGTGTCGCT CAAGGCGCAC TCCCGTTCTG GATAATGTTT
     2461 TTTGCGCCGA CATCATAACG GTTCTGGCAA ATATTCTGAA ATGAGCTGTT GACAATTAAT
     2521 CATCCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGA
     2581 CCATGGGCAT GAAAAAGATT TGGCTGGCGC TGGCTGGTTT AGTTTTAGCG TTTAGCGCAT
     2641 CGGCGTCTAG AGGCCGGCTC GGCCTTAAAA TCGAAGAAGG TAAACTGGTA ATCTGGATTA
     2701 ACGGCGATAA AGGCTATAAC GGTCTCGCTG AAGTCGGTAA GAAATTCGAG AAAGATACCG
     2761 GAATTAAAGT CACCGTTGAG CATCCGGATA AACTGGAAGA GAAATTCCCA CAGGTTGCGG
     2821 CAACTGGCGA TGGCCCTGAC ATTATCTTCT GGGCACACGA CCGCTTTGGT GGCTACGCTC
     2881 AATCTGGCCT GTTGGCTGAA ATCACCCCGG ACAAAGCGTT CCAGGACAAG CTGTATCCGT
     2941 TTACCTGGGA TGCCGTACGT TACAACGGCA AGCTGATTGC TTACCCGATC GCTGTTGAAG
     3001 CGTTATCGCT GATTTATAAC AAAGATCTGC TGCCGAACCC GCCAAAAACC TGGGAAGAGA
     3061 TCCCGGCGCT GGATAAAGAA CTGAAAGCGA AAGGTAAGAG CGCGCTGATG TTCAACCTGC
     3121 AAGAACCGTA CTTCACCTGG CCGCTGATTG CTGCTGACGG GGGTTATGCG TTCAAGTATG
     3181 AAAACGGCAA GTACGACATT AAAGACGTGG GCGTGGATAA CGCTGGCGCG AAAGCGGGTC
     3241 TGACCTTCCT GGTTGACCTG ATTAAAAACA AACACATGAA TGCAGACACC GATTACTCCA
     3301 TCGCAGAAGC TGCCTTTAAT AAAGGCGAAA CAGCGATGAC CATCAACGGC CCGTGGGCAT
     3361 GGTCCAACAT CGACACCAGC AAAGTGAATT ATGGTGTAAC GGTACTGCCG ACCTTCAAGG
     3421 GTCAACCATC CAAACCGTTC GTTGGCGTGC TGAGCGCAGG TATTAACGCC GCCAGTCCGA
     3481 ACAAAGAGCT GGCGAAAGAG TTCCTCGAAA ACTATCTGCT GACTGATGAA GGTCTGGAAG
     3541 CGGTTAATAA AGACAAACCG CTGGGTGCCG TAGCGCTGAA GTCTTACGAG GAAGAGTTGG
     3601 CGAAAGATCC ACGTATTGCC GCCACAATGG AAAACGCCCA GAAAGGTGAA ATCATGCCGA
     3661 ACATCCCGCA GATGTCCGCT TTCTGGTATG CCGTGCGTAC TGCGGTGATC AACGCCGCCA
     3721 GCGGTCGTCA GACTGTCGAT GAAGCCCTGA AAGACGCGCA GACTCGTATC ACCAAGCTCG
     3781 AGGATCAGAA CGCGACCGGC GGTGACCAAA ATGCCACAGG TGGCGATCAA AACGCCACCG
     3841 GCGGTGACCA GAATGCGACA GTCGACCATC ACCATCATCA CCATTAAAAG CTTGGCTGTT
     3901 TTGGCGGATG AGAGAAGATT TTCAGCCTGA TACAGATTAA ATCAGAACGC AGAAGCGGTC
     3961 TGATAAAACA GAATTTGCCT GGCGGCAGTA GCGCGGTGGT CCCACCTGAC CCCATGCCGA
     4021 ACTCAGAAGT GAAACGCCGT AGCGCCGATG GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG
     4081 GGAACTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT
     4141 ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG
     4201 AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG
     4261 CATCAAATTA AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT ACAAACTCTT
     4321 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
     4381 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
     4441 GACGTCGATA TCCGGATGAA GGCACGAACC CAGTGGACAT AAGCCTCGTT CGGTTCGTAA
     4501 GCTGTAATGC AAGTAGCGTA ACTGCCGTCA CGCAACTGGT CCACAACCTT GACCGAACGC
     4561 AGCGGTGGTA ACGGCGCAGT GGCGGTTTTC ATGGCTTCTT GTTATGACAT GTTTTTTTGG
     4621 GGTACAGTCT ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT
     4681 GATGTTATGG AGCAGCAACG ATGTTACGCA GCAGGGCAGT CGCCCTAAAA CAAAGTTAAA
     4741 CATCATGAGG GAAGCGGTGA TCGCCGAAGT ATCGACTCAA CTATCAGAGG TAGTTGGCGT
     4801 CATCGAGCGC CATCTCGAAC CGACGTTGCT GGCCGTACAT TTGTACGGCT CCGCAGTGGA
     4861 TGGCGGCCTG AAGCCACACA GTGATATTGA TTTGCTGGTT ACGGTGACCG TAAGGCTTGA
     4921 TGAAACAACG CGGCGAGCTT TGATCAACGA CCTTTTGGAA ACTTCGGCTT CCCCTGGAGA
     4981 GAGCGAGATT CTCCGCGCTG TAGAAGTCAC CATTGTTGTG CACGACGACA TCATTCCGTG
     5041 GCGTTATCCA GCTAAGCGCG AACTGCAATT TGGAGAATGG CAGCGCAATG ACATTCTTGC
     5101 AGGTATCTTC GAGCCAGCCA CGTACGACAT TGATCTGGCT ATCTTGCTGA CAAAAGCAAG
     5161 AGAACATAGC GTTGCCTTGG TAGGTCCAGC GGCGGAGGAA CTCTTTGATC CGGTTCCTGA
     5221 ACAGGATCTA TTTGAGGCGC TAAATGAAAC CTTAACGCTA TGGAACTCGC CGCCCGACTG
     5281 GGCTGGCGAT GAGCGAAATG TAGTGCTTAC GTTGTCCCGC ATTTGGTACA GCGCAGTAAC
     5341 CGGCAAAATC GCGCCGAAGG ATGTCGCTGC CGACTGGGCA ATGGAGCGCC TGCCGGCCCA
     5401 GTATCAGCCC GTCATACTTG AAGCTAGACA GGCTTATCTT GGACAAGAAG AAGATCGCTT
     5461 GGCCTCGCGC GCAGATCAGT TGGAAGAATT TGTCCACTAC GTGAAAGGCG AGATCACCAA
     5521 GGTAGTCGGC AAATAATGTC TAACAATTCG TTCAAGCCGA CGGATATCCT CGAGCTGAGA
     5581 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     5641 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     5701 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     5761 AAA     
//
