LOCUS       pTrc99S_ssDsbA_T        6006 bp ds-DNA     circular     12-JUL-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2667..4019
                     /locus_tag="C. tetani toxin fragment C domain"
                     /label="C. tetani toxin fragment C domain"
                     /ApEinfo_label="C. tetani toxin fragment C domain"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1..620
                     /vntifkey="33"
                     /locus_tag="pBR322 origin"
                     /label="pBR322 origin"
                     /ApEinfo_label="pBR322 origin"
                     /ApEinfo_fwdcolor="#fefe0a"
                     /ApEinfo_revcolor="#fefe0a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2589..2651
                     /locus_tag="DsbA signal sequence"
                     /label="DsbA signal sequence"
                     /ApEinfo_label="DsbA signal sequence"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2652..2666
                     /locus_tag="SfiI GGCCNNNN^NGGCC"
                     /label="SfiI GGCCNNNN^NGGCC"
                     /ApEinfo_label="SfiI GGCCNNNN^NGGCC"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2509..2582
                     /locus_tag="trc promoter"
                     /label="trc promoter"
                     /ApEinfo_label="trc promoter"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1186..2277
                     /locus_tag="LacI"
                     /label="LacI"
                     /ApEinfo_label="LacI"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4020..4103
                     /locus_tag="4xDQNAT"
                     /label="4xDQNAT"
                     /ApEinfo_label="4xDQNAT"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4068..4082
                     /locus_tag="DQNAT"
                     /label="DQNAT"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#fc8008"
                     /ApEinfo_revcolor="#fc8008"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4110..4127
                     /locus_tag="6xHis"
                     /label="6xHis"
                     /ApEinfo_label="6xHis"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4690..5811
                     /locus_tag="SpecR region"
                     /label="SpecR region"
                     /ApEinfo_label="SpecR region"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4219..4376
                     /locus_tag="rrnB terminator"
                     /label="rrnB terminator"
                     /ApEinfo_label="rrnB terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4026..4040
                     /locus_tag="DQNAT(1)"
                     /label="DQNAT(1)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4342..4385
                     /locus_tag="rrnB T1 terminator"
                     /label="rrnB T1 terminator"
                     /ApEinfo_label="rrnB T1 terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4047..4061
                     /locus_tag="DQNAT(2)"
                     /label="DQNAT(2)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4517..4544
                     /locus_tag="rrnB T2 terminator"
                     /label="rrnB T2 terminator"
                     /ApEinfo_label="rrnB T2 terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4089..4103
                     /locus_tag="DQNAT(3)"
                     /label="DQNAT(3)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4586..4614
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4131..4136
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
       61 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      121 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
      181 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      241 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      301 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      361 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      421 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      481 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      541 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      601 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
      661 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
      721 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
      781 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
      841 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC
      901 GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC
      961 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG
     1021 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCAGATCAA
     1081 TTCGCGCGCG AAGGCGAAGC GGCATGCATT TACGTTGACA CCATCGAATG GTGCAAAACC
     1141 TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA
     1201 CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC
     1261 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG
     1321 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG
     1381 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG
     1441 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC
     1501 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG
     1561 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT
     1621 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC
     1681 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC
     1741 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT
     1801 AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC
     1861 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG
     1921 CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG
     1981 CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT
     2041 ATCCCGCCGT CAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC
     2101 CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA
     2161 CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
     2221 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
     2281 CAACGCAATT AATGTGAGTT AGCGCGAATT GATCTGGTTT GACAGCTTAT CATCGACTGC
     2341 ACGGTGCACC AATGCTTCTG GCGTCAGGCA GCCATCGGAA GCTGTGGTAT GGCTGTGCAG
     2401 GTCGTAAATC ACTGCATAAT TCGTGTCGCT CAAGGCGCAC TCCCGTTCTG GATAATGTTT
     2461 TTTGCGCCGA CATCATAACG GTTCTGGCAA ATATTCTGAA ATGAGCTGTT GACAATTAAT
     2521 CATCCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGA
     2581 CCATGGGCAT GAAAAAGATT TGGCTGGCGC TGGCTGGTTT AGTTTTAGCG TTTAGCGCAT
     2641 CGGCGTCTAG AGGCCGGCTC GGCCTTAAGA ATCTGGATTG CTGGGTGGAC AATGAAGAAG
     2701 ACATCGACGT GATTCTTAAA AAGAGTACGA TCCTTAACTT GGATATTAAC AATGACATTA
     2761 TTTCAGACAT CTCTGGTTTC AATAGTTCAG TCATTACATA TCCGGATGCC CAACTGGTTC
     2821 CAGGAATCAA TGGAAAGGCG ATCCACCTTG TGAACAATGA GTCTAGCGAG GTGATTGTCC
     2881 ATAAGGCTAT GGACATCGAA TACAACGATA TGTTTAACAA CTTCACAGTA TCGTTTTGGC
     2941 TGCGTGTACC AAAAGTCTCA GCTTCTCATT TGGAACAATA CGATACAAAT GAATACTCCA
     3001 TCATCAGCTC TATGAAGAAG TATAGCCTTT CGATCGGCAG TGGTTGGTCC GTGTCCTTAA
     3061 AAGGTAATAA CTTAATCTGG ACATTAAAAG ATTCAGCCGG CGAAGTCCGT CAGATCACTT
     3121 TCCGCGACCT TTCCGATAAA TTTAACGCTT ACTTAGCAAA CAAATGGGTA TTCATTACCA
     3181 TCACAAACGA CCGTTTGAGC TCTGCCAATT TGTACATTAA CGGTGTTTTA ATGGGTAGCG
     3241 CAGAGATCAC GGGCCTTGGG GCTATCCGTG AGGACAATAA TATCACACTT AAGCTGGATC
     3301 GCTGCAATAA CAACAATCAA TATGTGAGCA TCGACAAATT TCGCATTTTT TGTAAGGCTC
     3361 TTAACCCCAA GGAGATCGAA AAGTTATATA CGAGTTACCT GAGCATTACC TTTCTGCGCG
     3421 ACTTTTGGGG GAATCCGTTA CGCTATGACA CAGAATATTA TTTAATCCCT GTGGCATACT
     3481 CTTCGAAAGA CGTCCAGCTG AAAAACATTA CCGACTATAT GTACTTAACG AACGCTCCAA
     3541 GCTACACGAA TGGAAAATTA AACATCTATT ACCGTCGTTT ATATTCTGGT CTGAAATTTA
     3601 TCATTAAACG CTATACACCC AATAATGAGA TTGATTCGTT CGTCCGTTCC GGTGACTTCA
     3661 TTAAGTTGTA TGTTTCGTAC AACAACAACG AACATATTGT TGGGTACCCC AAAGATGGAA
     3721 ACGCTTTCAA TAATTTGGAC CGCATCTTAC GTGTAGGCTA CAACGCTCCA GGGATCCCTC
     3781 TTTACAAAAA AATGGAAGCG GTAAAACTTC GCGACTTAAA AACTTATTCA GTCCAGCTTA
     3841 AGCTTTATGA CGATAAAGAT GCCTCGCTTG GCCTTGTTGG GACTCATAAC GGCCAAATCG
     3901 GTAATGACCC CAACCGCGAC ATCCTTATTG CATCCAATTG GTACTTTAAT CACCTGAAGG
     3961 ATAAGACTCT GACTTGCGAT TGGTACTTCG TACCGACTGA CGAAGGGTGG ACGAACGATC
     4021 TCGAGGATCA GAACGCGACC GGCGGTGACC AAAATGCCAC AGGTGGCGAT CAAAACGCCA
     4081 CCGGCGGTGA CCAGAATGCG ACAGTCGACC ATCACCATCA TCACCATTAA AAGCTTGGCT
     4141 GTTTTGGCGG ATGAGAGAAG ATTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG
     4201 GTCTGATAAA ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC
     4261 CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG
     4321 TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT
     4381 TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT
     4441 TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC
     4501 AGGCATCAAA TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT
     4561 CTTTTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
     4621 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG
     4681 CCCGACGTCG ATATCCGGAT GAAGGCACGA ACCCAGTGGA CATAAGCCTC GTTCGGTTCG
     4741 TAAGCTGTAA TGCAAGTAGC GTAACTGCCG TCACGCAACT GGTCCACAAC CTTGACCGAA
     4801 CGCAGCGGTG GTAACGGCGC AGTGGCGGTT TTCATGGCTT CTTGTTATGA CATGTTTTTT
     4861 TGGGGTACAG TCTATGCCTC GGGCATCCAA GCAGCAAGCG CGTTACGCCG TGGGTCGATG
     4921 TTTGATGTTA TGGAGCAGCA ACGATGTTAC GCAGCAGGGC AGTCGCCCTA AAACAAAGTT
     4981 AAACATCATG AGGGAAGCGG TGATCGCCGA AGTATCGACT CAACTATCAG AGGTAGTTGG
     5041 CGTCATCGAG CGCCATCTCG AACCGACGTT GCTGGCCGTA CATTTGTACG GCTCCGCAGT
     5101 GGATGGCGGC CTGAAGCCAC ACAGTGATAT TGATTTGCTG GTTACGGTGA CCGTAAGGCT
     5161 TGATGAAACA ACGCGGCGAG CTTTGATCAA CGACCTTTTG GAAACTTCGG CTTCCCCTGG
     5221 AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT CACCATTGTT GTGCACGACG ACATCATTCC
     5281 GTGGCGTTAT CCAGCTAAGC GCGAACTGCA ATTTGGAGAA TGGCAGCGCA ATGACATTCT
     5341 TGCAGGTATC TTCGAGCCAG CCACGTACGA CATTGATCTG GCTATCTTGC TGACAAAAGC
     5401 AAGAGAACAT AGCGTTGCCT TGGTAGGTCC AGCGGCGGAG GAACTCTTTG ATCCGGTTCC
     5461 TGAACAGGAT CTATTTGAGG CGCTAAATGA AACCTTAACG CTATGGAACT CGCCGCCCGA
     5521 CTGGGCTGGC GATGAGCGAA ATGTAGTGCT TACGTTGTCC CGCATTTGGT ACAGCGCAGT
     5581 AACCGGCAAA ATCGCGCCGA AGGATGTCGC TGCCGACTGG GCAATGGAGC GCCTGCCGGC
     5641 CCAGTATCAG CCCGTCATAC TTGAAGCTAG ACAGGCTTAT CTTGGACAAG AAGAAGATCG
     5701 CTTGGCCTCG CGCGCAGATC AGTTGGAAGA ATTTGTCCAC TACGTGAAAG GCGAGATCAC
     5761 CAAGGTAGTC GGCAAATAAT GTCTAACAAT TCGTTCAAGC CGACGGATAT CCTCGAGCTG
     5821 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC
     5881 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG
     5941 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG
     6001 TAGAAA     
//
