LOCUS       pTrc99S_ssDsbA_T        6021 bp ds-DNA     circular     12-JUL-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2589..2651
                     /locus_tag="DsbA signal sequence"
                     /label="DsbA signal sequence"
                     /ApEinfo_label="DsbA signal sequence"
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2652..2666
                     /locus_tag="SfiI GGCCNNNN^NGGCC"
                     /label="SfiI GGCCNNNN^NGGCC"
                     /ApEinfo_label="SfiI GGCCNNNN^NGGCC"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2509..2582
                     /locus_tag="trc promoter"
                     /label="trc promoter"
                     /ApEinfo_label="trc promoter"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1..620
                     /vntifkey="33"
                     /locus_tag="pBR322 origin"
                     /label="pBR322 origin"
                     /ApEinfo_label="pBR322 origin"
                     /ApEinfo_fwdcolor="#fefe0a"
                     /ApEinfo_revcolor="#fefe0a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1186..2277
                     /locus_tag="LacI"
                     /label="LacI"
                     /ApEinfo_label="LacI"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4234..4391
                     /locus_tag="rrnB terminator"
                     /label="rrnB terminator"
                     /ApEinfo_label="rrnB terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4035..4118
                     /locus_tag="4xDQNAT"
                     /label="4xDQNAT"
                     /ApEinfo_label="4xDQNAT"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4083..4097
                     /locus_tag="DQNAT"
                     /label="DQNAT"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#fc8008"
                     /ApEinfo_revcolor="#fc8008"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4125..4142
                     /locus_tag="6xHis"
                     /label="6xHis"
                     /ApEinfo_label="6xHis"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4705..5826
                     /locus_tag="SpecR region"
                     /label="SpecR region"
                     /ApEinfo_label="SpecR region"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4041..4055
                     /locus_tag="DQNAT(1)"
                     /label="DQNAT(1)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4357..4400
                     /locus_tag="rrnB T1 terminator"
                     /label="rrnB T1 terminator"
                     /ApEinfo_label="rrnB T1 terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4062..4076
                     /locus_tag="DQNAT(2)"
                     /label="DQNAT(2)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4532..4559
                     /locus_tag="rrnB T2 terminator"
                     /label="rrnB T2 terminator"
                     /ApEinfo_label="rrnB T2 terminator"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4104..4118
                     /locus_tag="DQNAT(3)"
                     /label="DQNAT(3)"
                     /ApEinfo_label="DQNAT"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4601..4629
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4146..4151
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2667..4034
                     /locus_tag="C. tetani light chain domain"
                     /label="C. tetani light chain domain"
                     /ApEinfo_label="C. tetani light chain domain"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3363..3365
                     /locus_tag="E234A mutation"
                     /label="E234A mutation"
                     /ApEinfo_label="E234A mutation"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
       61 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      121 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
      181 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      241 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      301 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      361 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      421 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      481 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      541 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      601 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
      661 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
      721 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
      781 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
      841 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC
      901 GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC
      961 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG
     1021 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCAGATCAA
     1081 TTCGCGCGCG AAGGCGAAGC GGCATGCATT TACGTTGACA CCATCGAATG GTGCAAAACC
     1141 TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA
     1201 CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC
     1261 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG
     1321 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG
     1381 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG
     1441 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC
     1501 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG
     1561 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT
     1621 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC
     1681 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC
     1741 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT
     1801 AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC
     1861 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG
     1921 CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG
     1981 CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT
     2041 ATCCCGCCGT CAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC
     2101 CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA
     2161 CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
     2221 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
     2281 CAACGCAATT AATGTGAGTT AGCGCGAATT GATCTGGTTT GACAGCTTAT CATCGACTGC
     2341 ACGGTGCACC AATGCTTCTG GCGTCAGGCA GCCATCGGAA GCTGTGGTAT GGCTGTGCAG
     2401 GTCGTAAATC ACTGCATAAT TCGTGTCGCT CAAGGCGCAC TCCCGTTCTG GATAATGTTT
     2461 TTTGCGCCGA CATCATAACG GTTCTGGCAA ATATTCTGAA ATGAGCTGTT GACAATTAAT
     2521 CATCCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGA
     2581 CCATGGGCAT GAAAAAGATT TGGCTGGCGC TGGCTGGTTT AGTTTTAGCG TTTAGCGCAT
     2641 CGGCGTCTAG AGGCCGGCTC GGCCTTCCTA TCACCATCAA TAACTTCCGC TACTCAGATC
     2701 CAGTGAACAA CGACACTATT ATTATGATGG AACCCCCCTA TTGCAAGGGA TTAGATATCT
     2761 ATTATAAAGC CTTCAAGATT ACCGATCGTA TCTGGATTGT CCCCGAGCGC TACGAATTCG
     2821 GGACCAAACC CGAAGATTTT AATCCACCAA GCAGCTTGAT CGAGGGTGCT TCGGAGTACT
     2881 ATGATCCCAA CTACCTGCGT ACCGATTCGG ATAAAGACCG TTTTCTGCAG ACGATGGTAA
     2941 AACTTTTCAA TCGTATTAAG AATAATGTCG CGGGCGAAGC CTTATTGGAT AAGATTATCA
     3001 ATGCGATTCC TTATCTTGGC AACAGTTACT CGTTACTGGA CAAGTTTGAT ACGAATTCAA
     3061 ATAGCGTGAG TTTTAACCTG TTGGAACAGG ACCCTTCAGG CGCGACCACT AAAAGCGCAA
     3121 TGTTAACCAA CTTAATCATT TTTGGACCCG GTCCCGTATT GAACAAAAAT GAGGTTCGCG
     3181 GGATCGTACT GCGTGTAGAC AATAAGAATT ACTTTCCGTG CCGCGATGGC TTTGGCTCGA
     3241 TTATGCAAAT GGCGTTTTGC CCAGAGTACG TGCCAACATT CGACAATGTC ATCGAAAATA
     3301 TCACCTCTCT GACCATCGGA AAGAGTAAAT ATTTCCAAGA TCCTGCTTTG TTGCTGATGC
     3361 ATGCCCTGAT CCATGTGTTA CATGGTTTAT ACGGTATGCA AGTTTCTTCG CACGAAATTA
     3421 TTCCCTCGAA ACAGGAAATT TACATGCAAC ATACATACCC GATTAGTGCG GAGGAGCTGT
     3481 TCACCTTTGG CGGGCAGGAC GCGAATTTAA TCTCTATTGA TATCAAGAAC GACTTATATG
     3541 AAAAGACATT GAACGACTAC AAAGCAATCG CAAACAAACT GAGTCAGGTC ACCTCATGCA
     3601 ATGACCCGAA CATTGATATT GACAGTTATA AGCAGATCTA CCAACAAAAA TACCAATTTG
     3661 ATAAGGATTC TAATGGTCAA TATATTGTGA ACGAGGATAA GTTTCAGATT CTTTATAATA
     3721 GCATCATGTA TGGTTTTACT GAGATTGAGC TGGGGAAAAA ATTCAACATT AAAACCCGTC
     3781 TTTCGTATTT TTCGATGAAC CACGATCCCG TTAAAATTCC GAACTTACTG GATGACACTA
     3841 TCTATAACGA CACCGAAGGA TTTAACATTG AGAGCAAGGA TCTGAAGTCT GAATACAAGG
     3901 GCCAAAACAT GCGCGTAAAT ACGAATGCCT TCCGCAATGT CGATGGATCG GGACTGGTGA
     3961 GCAAATTGAT CGGCTTATGT AAGAAGATTA TCCCGCCTAC AAACATCCGT GAGAACTTAT
     4021 ACAACCGCAC TGCCCTCGAG GATCAGAACG CGACCGGCGG TGACCAAAAT GCCACAGGTG
     4081 GCGATCAAAA CGCCACCGGC GGTGACCAGA ATGCGACAGT CGACCATCAC CATCATCACC
     4141 ATTAAAAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA CAGATTAAAT
     4201 CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC
     4261 CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT
     4321 CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
     4381 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT
     4441 CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC
     4501 CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT
     4561 GCGTTTCTAC AAACTCTTTT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
     4621 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
     4681 AACATTTCCG TGTCGCCCGA CGTCGATATC CGGATGAAGG CACGAACCCA GTGGACATAA
     4741 GCCTCGTTCG GTTCGTAAGC TGTAATGCAA GTAGCGTAAC TGCCGTCACG CAACTGGTCC
     4801 ACAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTCTTGT
     4861 TATGACATGT TTTTTTGGGG TACAGTCTAT GCCTCGGGCA TCCAAGCAGC AAGCGCGTTA
     4921 CGCCGTGGGT CGATGTTTGA TGTTATGGAG CAGCAACGAT GTTACGCAGC AGGGCAGTCG
     4981 CCCTAAAACA AAGTTAAACA TCATGAGGGA AGCGGTGATC GCCGAAGTAT CGACTCAACT
     5041 ATCAGAGGTA GTTGGCGTCA TCGAGCGCCA TCTCGAACCG ACGTTGCTGG CCGTACATTT
     5101 GTACGGCTCC GCAGTGGATG GCGGCCTGAA GCCACACAGT GATATTGATT TGCTGGTTAC
     5161 GGTGACCGTA AGGCTTGATG AAACAACGCG GCGAGCTTTG ATCAACGACC TTTTGGAAAC
     5221 TTCGGCTTCC CCTGGAGAGA GCGAGATTCT CCGCGCTGTA GAAGTCACCA TTGTTGTGCA
     5281 CGACGACATC ATTCCGTGGC GTTATCCAGC TAAGCGCGAA CTGCAATTTG GAGAATGGCA
     5341 GCGCAATGAC ATTCTTGCAG GTATCTTCGA GCCAGCCACG TACGACATTG ATCTGGCTAT
     5401 CTTGCTGACA AAAGCAAGAG AACATAGCGT TGCCTTGGTA GGTCCAGCGG CGGAGGAACT
     5461 CTTTGATCCG GTTCCTGAAC AGGATCTATT TGAGGCGCTA AATGAAACCT TAACGCTATG
     5521 GAACTCGCCG CCCGACTGGG CTGGCGATGA GCGAAATGTA GTGCTTACGT TGTCCCGCAT
     5581 TTGGTACAGC GCAGTAACCG GCAAAATCGC GCCGAAGGAT GTCGCTGCCG ACTGGGCAAT
     5641 GGAGCGCCTG CCGGCCCAGT ATCAGCCCGT CATACTTGAA GCTAGACAGG CTTATCTTGG
     5701 ACAAGAAGAA GATCGCTTGG CCTCGCGCGC AGATCAGTTG GAAGAATTTG TCCACTACGT
     5761 GAAAGGCGAG ATCACCAAGG TAGTCGGCAA ATAATGTCTA ACAATTCGTT CAAGCCGACG
     5821 GATATCCTCG AGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
     5881 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG
     5941 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT
     6001 GAGCGTCAGA CCCCGTAGAA A   
//
