LOCUS       Exported                5028 bp ds-DNA     circular SYN 15-APR-2019
DEFINITION  Expression vector for pmTurq2-Golgi.
ACCESSION   .
VERSION     .
KEYWORDS    pCS2+pmTurquoise2-Golgi
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Rusty Lansford
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 7, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="recombinant plasmid"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    98..280
                     /label=hGalT
     CDS             320..1029
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDE"
     CDS             1030..1039
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="YK"
     misc_feature    1050..1077
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     polyA_signal    1077..1211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1155..1174
                     /label=SV40pA-R
     primer_bind     complement(1322..1341)
                     /label=T3
     promoter        complement(1324..1342)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1363..1379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1363..1379)
                     /label=M13R
     protein_bind    1387..1403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1411..1441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(1765..2353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2524..3384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3385..3489)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3515..3970)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4044..5028
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atctcgagct cgccaccatg aggcttcggg agccgctcct
      121 gagcggcagc gccgcgatgc caggcgcgtc cctacagcgg gcctgccgcc tgctcgtggc
      181 cgtctgcgct ctgcaccttg gcgtcaccct cgtttactac ctggctggcc gcgacctgag
      241 ccgcctgccc caactggtcg gagtctccac accgctgcag tcgacggtac cgcgggcccg
      301 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      361 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      421 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      481 gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg
      541 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      601 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      661 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      721 cggcaacatc ctggggcaca agctggagta caactacttt agcgacaacg tctatatcac
      781 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
      841 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      901 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
      961 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1021 ggacgagctg tacaagtaag tcgagcctct agaactatag tgagtcgtat tacgtagatc
     1081 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1141 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     1201 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     1261 gggaggtttt ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg
     1321 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     1381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1561 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2461 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2521 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2581 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2641 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2701 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2761 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2821 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2881 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2941 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3001 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3061 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3121 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3181 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3241 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3301 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3361 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3421 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3481 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     3541 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3601 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3661 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3721 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3781 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3841 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3901 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3961 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4021 gcctcttcgc tattacgcca gtcgaccata gccaattcaa tatggcgtat atggactcat
     4081 gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt
     4141 gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat
     4201 gccaattcaa tatggcggac ttggcactgt gccaactggg gaggggtcta cttggcacgg
     4261 tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg
     4321 tgccaataat ggcggccata ttggctatat gccaggatca atatataggc aatatccaat
     4381 atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca
     4441 tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc
     4501 ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa
     4561 tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg
     4621 gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac
     4681 gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt
     4741 tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat
     4801 ctattaatag taacttggca agtacattac tattggaagg acgccagggt acattggcag
     4861 tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat
     4921 tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt
     4981 aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaac
//
