LOCUS       Exported                5081 bp ds-DNA     circular SYN 15-APR-2019
DEFINITION  Expression vector for FucciOrange.
ACCESSION   .
VERSION     .
KEYWORDS    pCS2+FucciOrange
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Rusty Lansford
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 7, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="recombinant plasmid"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     primer_bind     34..52
                     /label=SP6
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             111..1088
                     /codon_start=1
                     /label=Fucci Orange
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHYSMLP
                     SQGGYPSHWRPLEPSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSVD
                     EVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     misc_feature    1089..1093
                     /label=P2A
     misc_feature    1103..1130
                     /label=stop codons
                     /note="stop codons in all three reading frames"
     polyA_signal    1130..1264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1208..1227
                     /label=SV40pA-R
     primer_bind     1298..1314
                     /label=EGFP-C-REV
     primer_bind     complement(1375..1394)
                     /label=T3
     promoter        complement(1377..1395)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1416..1432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1416..1432)
                     /label=M13R
     primer_bind     complement(1430..1449)
                     /label=M13-48REV
     protein_bind    1440..1456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1464..1494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(1818..2406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2577..3437)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3438..3542)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3568..4023)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4097..5081
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     primer_bind     5023..5040
                     /label=CMV-Forward
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattct gcaggccacc atggtgagtg
      121 tgattaaacc agagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt
      181 tcacaattga aggtgaaggc acaggcagac cttacgaggg acatcaagag atgacactac
      241 gcgtcacaat ggccgagggc gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt
      301 tctgttacgg ccacagagta tttactaaat atccagaaga gataccagac tatttcaaac
      361 aagcatttcc tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg
      421 cttcagtcag tgcgcatata agccttagag gaaacacctt ctaccacaaa tccaaattta
      481 ctggggttaa ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc
      541 catcaaccga gaaaattact gccagcgacg gagttctgaa gggtgatgtt acgatgtacc
      601 taaaacttga aggaggcggc aatcacaaat gccaaatgaa gactacttac aaggcggcaa
      661 aagagattct tgaaatgcca ggagaccatt acatcggcca tcgcctcgtc aggaaaaccg
      721 aaggcaacat tactgagcag gtagaagatg cagtagctca ttactcaatg ttgccttccc
      781 agggcggata tccatcacac tggcggccgc tcgagcccag ccccgccagg cccgcactcc
      841 gcgccccggc ctccgctacc agtggcagcc gcaagcgcgc ccgcccgccc gccgcccccg
      901 gacgcgacca ggccaggcca ccggcccgca ggagactgcg gctgtcggtg gacgaggttt
      961 ccagccccag tacccccgag gccccagaca tcccagcctg cccttctccg ggccagaaga
     1021 taaagaaatc caccccggca gcaggtcagc cgccccacct gacatccgcg caggaccagg
     1081 acaccatcgg atctcgagcc tctagaacta tagtgagtcg tattacgtag atccagacat
     1141 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     1201 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     1261 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     1321 tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt ttgttccctt
     1381 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1441 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1561 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2221 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3181 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3541 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     3601 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3661 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3721 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3781 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3841 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3901 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3961 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4021 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     4081 cgctattacg ccagtcgacc atagccaatt caatatggcg tatatggact catgccaatt
     4141 caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact cgtgccaatt
     4201 caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac catgccaatt
     4261 caatatggcg gacttggcac tgtgccaact ggggaggggt ctacttggca cggtgccaag
     4321 tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca tggtgccaat
     4381 aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc aatatggccc
     4441 tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg ccatatagta
     4501 ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg gtcccatata
     4561 ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt caatggtctc
     4621 tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat atggcgcctc
     4681 ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt gacgtcaata
     4741 ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc cgttctcacg
     4801 ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa tatctattaa
     4861 tagtaacttg gcaagtacat tactattgga aggacgccag ggtacattgg cagtactccc
     4921 attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc cattgacgtc
     4981 aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg
     5041 cctaatggga ggtctatata agcaatgctc gtttagggaa c
//
