LOCUS Exported 6880 bp ds-DNA circular SYN 06-JUN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6880) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Jun 24, 2019 from SnapGene Viewer 4.3.10 https://www.snapgene.com COMMENT Ligation of : pMinDisEnhancedExport-X513-L121G4-new.xdna [5357 nt] : (PpuMI[2319] / BglII[800]) to PCR(pRSET-X513-L194D1.xdna) [1516 nt] : (BglII[11] / PpuMI[1530]) FEATURES Location/Qualifiers source 1..6880 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 10..389 /label=human cytomegalovirus immediate early enhancer_ /label=human cytomegalovirus immediate early enhancer_... /note="note=""CMV enhancer"" - note=""human cytomegalovirus immediate early enhancer"" - 10..389" promoter 390..593 /label=human cytomegalovirus (CMV) immediate early _CM /label=human cytomegalovirus (CMV) immediate early _CM... /note="note=""CMV promoter"" - note=""human cytomegalovirus (CMV) immediate early - promoter"" - 390..593" promoter 638..656 /label=promoter for bacteriophage T7 RNA polymerase_T7 /label=promoter for bacteriophage T7 RNA polymerase_T7... /note="note=""T7 promoter"" - note=""promoter for bacteriophage T7 RNA polymerase"" - 638..656" CDS 737..799 /label=Ig-kappa leader_leader sequence from mouse_CDS /note="codon_start=1 - note=""leader sequence from mouse"" - note=""Ig-kappa leader"" - translation=""METDTLLLWVLLLWVPGSTGD"" - 737..799" CDS 737..739 /codon_start=1 /label=Start /translation="M" misc_feature 806..1030 /label=X513OpuB 30-106 /note="note=""X513OpuB 30-106"" - 805..1029" misc_feature 1031..1042 /label=Linker (N) misc_feature 1043..1765 /label=cpsfGFP misc_feature 1766..1777 /label=Linker (C) misc_feature 1778..2368 /label=X513OpuB 107-305 /note="note=""X513OpuB 107-305"" - 1774..2367" CDS 2375..2404 /label=Myc_Myc (human c-Myc oncogene) epitope tag_CDS /note="codon_start=1 - note=""Myc (human c-Myc oncogene) epitope tag"" - note=""Myc"" - translation=""EQKLISEEDL"" - 819..848" misc_feature 2471..2539 /label=PDGFR transmembrane helix /note="note=""PDGFR transmembrane helix"" - 915..983" misc_feature 2531..2608 /label=ER Export Motifs_perLL123016 /note="note=""ProposedExportMotifs_perLL_123016"" - 975..1052" polyA_signal 2636..2860 /label=bovine growth hormone polyadenylation signal_bG /label=bovine growth hormone polyadenylation signal_bG... /note="note=""bGH poly(A) signal"" - note=""bovine growth hormone polyadenylation signal"" - 1080..1304" rep_origin complement(2992..3580) /direction=LEFT /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o... /note="direction=LEFT - note=""LEFT"" - note=""high-copy-number ColE1/pMB1/pBR322/pUC origin of - replication"" - complement(1436..2024)" polyA_signal 3909..3956 /label=herpes simplex virus thymidine kinase _HSV TK p /label=herpes simplex virus thymidine kinase _HSV TK p... /note="note=""HSV TK poly(A) signal"" - note=""herpes simplex virus thymidine kinase - polyadenylation signal"" - 2353..2400" promoter complement(5011..5371) /label=SV40 enhancer and early promoter_SV40 promoter_ /label=SV40 enhancer and early promoter_SV40 promoter_... /note="note=""SV40 promoter"" - note=""SV40 enhancer and early promoter"" - complement(3455..3815)" rep_origin 5025..5163 /direction=RIGHT /label=SV40 origin of replication_SV40 ori_rep_origin /note="direction=RIGHT - note=""SV40 ori"" - note=""SV40 origin of replication"" - 3469..3607" CDS complement(5431..6291) /label=confers resistance to ampicillin, carbenicillin /label=confers resistance to ampicillin, carbenicillin... /note="codon_start=1 - note=""bla"" - note=""confers resistance to ampicillin, carbenicillin, and - related antibiotics"" - translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS - PVTEKHLTDGMTVRELCSA" promoter complement(6292..6396) /label=AmpR promoter_bla_promoter /note="note=""bla"" - note=""AmpR promoter"" - complement(4736..4840)" rep_origin 6423..6878 /direction=RIGHT /label=f1 bacteriophage origin of replication /label=f1 bacteriophage origin of replication; arrow _... /note="direction=RIGHT - note=""RIGHT"" - note=""f1 bacteriophage origin of replication; arrow - indicates direction of (+) strand synthesis"" - 4867..5322" ORIGIN 1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg 721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt 781 tccaggttcc actggtgaca gatctgcgaa cgacaccgta gttgtgggct cgattaacca 841 tacagaacag attatcgtcg caaacatgtt ggcagagatg attgaggcgc atacagacct 901 taaggtggtt cgcaaactga accttggcgg ggttaacgtt aactttgaag ccattaaacg 961 cggaggtgcg aataatggta ttgacattta cctggagtac gttgggtatg gtcttgtgga 1021 tattctgggg tacccggagc cgaacgtcta tatcatcgcc gacaagcaga agaacggcat 1081 caaggcgaac ttcaagatcc gctacaacgt ggaggacggc agcgtgcagc tcgccgacca 1141 ctaccagcag aacaccccca tcggcgacgg ccctgtgctg ctgcctgaca accactacct 1201 gagcacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 1261 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac 1321 cggagggagc atgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 1381 gctggacggc ggcgtaaacg gccacaagtt cagcgtgcgc ggcgagggcg agggcgatgc 1441 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 1501 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 1561 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 1621 catcagcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga 1681 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 1741 ggggcacaag ctggagtaca actttccgcc gcccggtgct actgatccag aaggtgcata 1801 cgaaaccgtg aagaaggagt acaaacgtaa atggaatatt gtatggctca aaccactggg 1861 attcaacgct tcgtatgtgc ttgcggttaa agacgaactg gcgaaacagt ataaccttaa 1921 aaccttcagt gacttagcga aaatctcgga taagctgatt ctgggtgcaa acatgatgtt 1981 tttagaaaat cccgatggtt acccaggcct gcaaaaactg tacaatttca aattcaagca 2041 caccaaaagc atggacgctg gtattccgta taccgccatt gataataacg aagttcaggt 2101 aattgatgcc actgccactg atggcttgct ggtgagccac aaattaaaaa ttctggagga 2161 tgataaagcg ttcttcccgc cgtattatgc tgcccccatc atccgtcagg atgtcttaga 2221 taagcatcct gaactgaagg acgtgctgaa caaactcgcg aatcaaattt cactggaaga 2281 aatgcagaaa ctgaattaca agcgtgacgg tgagggtcag gacccagcga aagtagctaa 2341 ggagtttttg aaagagaaag gtttaattac gcgtgaacaa aaactcatct cagaagagga 2401 tctgaatgct gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt 2461 taaggtggtg gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat 2521 catcctcatc aaatacttgc agaagaggcg ggaaaggcga cgccaggaga acgctaactc 2581 cttttgctat gaaaatgagg tagccctgta ggcggccgct cgagatcagc ctcgactgtg 2641 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 2701 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 2761 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 2821 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 2881 agtggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 2941 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 3001 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3061 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3121 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 3181 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3241 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 3301 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 3361 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 3421 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 3481 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 3541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 3601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 3661 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 3721 aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag 3781 ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta 3841 ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc 3901 gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat 3961 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg 4021 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa 4081 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg 4141 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg 4201 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg 4261 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct 4321 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg 4381 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg 4441 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc 4501 tcttgatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 4561 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 4621 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 4681 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 4741 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 4801 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 4861 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 4921 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga 4981 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc 5041 tcctcactac ttctggaata gctcagaggc cgaggaggcg gcctcggcct ctgcataaat 5101 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg 5161 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat 5221 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg 5281 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca 5341 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata aatcaatcta 5401 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 5461 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 5521 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 5581 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 5641 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 5701 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 5761 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 5821 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 5881 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 5941 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6001 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 6061 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 6121 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 6181 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 6241 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 6301 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 6361 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 6421 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 6481 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 6541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 6601 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 6661 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 6721 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 6781 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 6841 taacgcgaat tttaacaaaa tattaacgct tacaatttac //