LOCUS Exported 7410 bp ds-DNA circular SYN 06-JUN-2019 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7410) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jun 6, 2019 from SnapGene Viewer 4.3.8 https://www.snapgene.com COMMENT Ligation of : pAAV-CAG-YMAT-Nlgn.xdna [5837 nt] : (BamHI[3114] / EcoRI[1545]) to PCR(pMinDisEnhancedExport-X513-L121G4-new) [Backup 2018-11-27 15_13_31_3:13 PM] [1567 nt] : (EcoRI[6] / BamHI[1577]) FEATURES Location/Qualifiers source 1..7410 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding activates transcription in the pres... /note="note=""E. coli catabolite activator protein"" - note=""CAP binding activates transcription in the presence - of cAMP."" - 107..128" /note="color: #c0c0c0; direction: RIGHT" promoter 143..173 /label=promoter for the E. coli lac operon_lac promote... /note="note=""lac promoter"" - note=""promoter for the E. coli lac operon"" - 143..173" /note="color: #c0c0c0; direction: RIGHT" protein_bind 181..197 /label=The lac repressor binds to the lac operator to ... /note="note=""lac repressor encoded by lacI"" - note=""The lac repressor binds to the lac operator to - inhibit transcription in E. coli. This inhibition can be - relieved by adding lactose or - isopropyl-beta-D-thiogalactopyranoside (IPTG)."" - 181..197" /note="color: #c0c0c0; direction: RIGHT" misc_feature 259..426 /label=ITR_misc_feature /note="note=""ITR"" - 259..426" /note="color: #80ffff; direction: RIGHT" promoter 451..1385 /label=CBA promoter_promoter /note="note=""CBA promoter"" - 451..1385" /note="color: #c0c0c0; direction: RIGHT" misc_feature 1256..1278 /label=pCAG-N 0.03 /note="color: #9b43df; direction: RIGHT" misc_feature 1401..1538 /label=Signal peptide /note="color: #529cff; direction: RIGHT" misc_feature 1551..1775 /label=X513OpuB 30-106 /note="note=""X513OpuB 30-106"" - 805..1029" /note="color: #dc143c; direction: RIGHT" misc_feature 1776..1787 /label=Linker (N) /note="color: #0378ff; direction: RIGHT" misc_feature 1788..2510 /label=cpsfGFP /note="color: #5fff45; direction: RIGHT" misc_feature 2511..2519 /label=Linker (C) /note="color: #0378ff; direction: RIGHT" misc_feature 2520..3113 /label=X513OpuB 107-305 /note="note=""X513OpuB 107-305"" - 1774..2367" /note="color: #dc143c; direction: RIGHT" misc_feature 3120..3332 /label=neuroligin extracellular /note="color: #84a4c0; direction: RIGHT" misc_feature 3333..3392 /label=transmembrane predicted /note="color: #84a4c0; direction: RIGHT" CDS complement(3387..3398) /label=FactorXa site /note="color: #c0c0c0; direction: LEFT" misc_feature 3393..3773 /label=neuroligin intracellular /note="color: #84a4c0; direction: RIGHT" misc_feature 3774..3791 /label=Linker stop /note="color: #84a4c0; direction: RIGHT" misc_feature 3804..4392 /label=WPRE_misc_feature /note="note=""WPRE"" - 2764..3352" /note="color: #c0c0c0; direction: RIGHT" misc_feature 3940..3959 /label=pCAG-C 0.02 /note="color: #9b43df; direction: RIGHT" CDS complement(4275..4286) /label=Factor Xa site_Factor Xa recognition and cleava... /note="codon_start=1 - note=""Factor Xa recognition and cleavage site"" - note=""Factor Xa site"" - translation=""IEGR"" - complement(3235..3246)" /note="color: #c0c0c0; direction: LEFT" misc_feature 4420..4652 /label=SV40_misc_feature /note="note=""SV40"" - 3380..3612" /note="color: #c0c0c0; direction: RIGHT" misc_feature complement(4680..4847) /label=ITR_misc_feature /note="note=""ITR"" - 3640..3807" /note="color: #6ef8ff; direction: LEFT" rep_origin 5024..5479 /direction=RIGHT /label=f1 bacteriophage origin of replication; arrow _... /note="direction=RIGHT - note=""RIGHT"" - note=""f1 bacteriophage origin of replication; arrow - indicates direction of (+) strand synthesis"" - 3984..4439" /note="color: #c0c0c0" promoter 5505..5609 /label=AmpR promoter_bla_promoter /note="note=""bla"" - note=""AmpR promoter"" - 4465..4569" /note="color: #c0c0c0; direction: RIGHT" CDS 5610..6470 /label=confers resistance to ampicillin, carbenicillin... /note="codon_start=1 - note=""bla"" - note=""confers resistance to ampicillin, carbenicillin, and - related antibiotics"" - translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS - PVTEKHLTDGMTVRELCSA" /note="color: #c0c0c0; direction: RIGHT" rep_origin 6641..7229 /direction=RIGHT /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o... /note="direction=RIGHT - note=""RIGHT"" - note=""high-copy-number ColE1/pMB1/pBR322/pUC origin of - replication"" - 5601..6189" /note="color: #c0c0c0" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta 241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc 301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga 361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta 421 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg 721 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta 781 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg 841 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg 901 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg 961 aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg 1021 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc 1081 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt 1141 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg 1201 gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc 1261 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc 1321 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa 1381 gaattggatc tgccgccacc atggcacttc ctagatgtat gtggcctaat tatgtatggc 1441 gagctatgat ggcctgcgtg gtgcaccgag ggagtggcgc cccacttaca ctctgcctgt 1501 tgggttgcct cctccagact ttccacgttc tttcacagaa gctggaattc gcgaacgaca 1561 ccgtagttgt gggctcgatt aaccatacag aacagattat cgtcgcaaac atgttggcag 1621 agatgattga ggcgcataca gaccttaagg tggttcgcaa actgaacctt ggcggggtta 1681 acgttaactt tgaagccatt aaacgcggag gtgcgaataa tggtattgac atttacctgg 1741 agtacgttgg gtatggtctt gtggatattc tggggtaccc ggagccgaac gtctatatca 1801 tcgccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgctac aacgtggagg 1861 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccctg 1921 tgctgctgcc tgacaaccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg 1981 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 2041 tggacgagct gtacaagggc ggtaccggag ggagcatgag caagggcgag gagctgttca 2101 ccggggtggt gcccatcctg gtcgagctgg acggcggcgt aaacggccac aagttcagcg 2161 tgcgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca 2221 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 2281 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 2341 ccgaaggcta cgtccaggag cgcaccatca gcttcaagga cgacggcacc tacaagaccc 2401 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 2461 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttt ccgccgcccg 2521 gtgctactga tccagaaggt gcatacgaaa ccgtgaagaa ggagtacaaa cgtaaatgga 2581 atattgtatg gctcaaacca ctgggattca acgcttcgta tgtgcttgcg gttaaagacg 2641 aactggcgaa acagtataac cttaaaacct tcagtgactt agcgaaaatc tcggataagc 2701 tgattctggg tgcaaacatg atgtttttag aaaatcccga tggttaccca ggcctgcaaa 2761 aactgtacaa tttcaaattc aagcacacca aaagcatgga cgctggtatt ccgtataccg 2821 ccattgataa taacgaagtt caggtaattg atgccactgc cactgatggc ttgctggtga 2881 gccacaaatt aaaaattctg gaggatgata aagcgttctt cccgccgtat tatgctgccc 2941 ccatcatccg tcaggatgtc ttagataagc atcctgaact gaaggacgtg ctgaacaaac 3001 tcgcgaatca aatttcactg gaagaaatgc agaaactgaa ttacaagcgt gacggtgagg 3061 gtcaggaccc agcgaaagta gctaaggagt ttttgaaaga gaaaggttta attggatcca 3121 cgctcgagct ggtgccccac ttgcataacc tcaatgacat cagccaatac acaagtacaa 3181 ccaccaaggt cccaagcact gacatcaccc ttcgaccaac tcggaaaaac tcaacccctg 3241 tgaccagcgc atttccaacc gctaagcaag acgaccccaa acaacagcct agtccattta 3301 gcgtagacca aagggactac agcacagagc tgtccgtgac catagctgta ggagcttccc 3361 tgcttttcct gaacattctg gctttcgcgg ccctctatta caagaaggac aaaaggcgcc 3421 acgacgtaca taggagatgt agtccgcaac gaactacaac caatgatctt acgcacgcac 3481 ctgaggagga gatcatgagt cttcagatga aacacacaga cctggaccac gagtgtgaat 3541 ccatccatcc ccatgaggtg gtgctgagga cagcatgccc tccggactat accctggcaa 3601 tgcgccgaag cccagacgac attcccctga tgacacccaa taccataacc atgattccca 3661 acactattcc aggaatccag cctctccaca ctttcaacac attcactggt ggacagaata 3721 atactctccc tcatccgcat ccacaccccc actcccacag cacaacacgg gtgggtggca 3781 gcggtggcta aaagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 3841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 3901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 3961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 4081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 4141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 4201 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 4261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 4321 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 4381 ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat 4441 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 4501 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 4561 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa 4621 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac 4681 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg 4741 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 4801 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct 4861 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 4921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 4981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 5041 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 5101 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 5161 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 5221 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 5281 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 5341 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 5401 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 5461 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 5521 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 5581 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 5641 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 5701 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 5761 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 5821 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 5881 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 5941 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 6001 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 6061 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 6121 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 6181 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 6241 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 6301 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 6361 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 6421 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 6481 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 6541 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 6601 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 6661 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 6721 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 6781 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 6841 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 6901 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 6961 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 7021 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 7081 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 7141 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 7201 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 7261 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 7321 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 7381 gcagcgagtc agtgagcgag gaagcggaag //