LOCUS       Exported                7410 bp ds-DNA     circular SYN 06-JUN-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 6, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
COMMENT     Ligation of : pAAV-CAG-YMAT-Nlgn.xdna [5837 nt] : (BamHI[3114] / 
            EcoRI[1545]) to PCR(pMinDisEnhancedExport-X513-L121G4-new) [Backup 
            2018-11-27 15_13_31_3:13 PM] [1567 nt] : (EcoRI[6] / BamHI[1577])
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding activates transcription in the pres...
                     /note="note=""E. coli catabolite activator protein"" - 
                     note=""CAP binding activates transcription in the presence 
                     - of cAMP."" - 107..128"
                     /note="color: #c0c0c0; direction: RIGHT"
     promoter        143..173
                     /label=promoter for the E. coli lac operon_lac promote...
                     /note="note=""lac promoter"" - note=""promoter for the E. 
                     coli lac operon"" - 143..173"
                     /note="color: #c0c0c0; direction: RIGHT"
     protein_bind    181..197
                     /label=The lac repressor binds to the lac operator to ...
                     /note="note=""lac repressor encoded by lacI"" - note=""The 
                     lac repressor binds to the lac operator to - inhibit 
                     transcription in E. coli. This inhibition can be - relieved
                     by adding lactose or - 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."" - 
                     181..197"
                     /note="color: #c0c0c0; direction: RIGHT"
     misc_feature    259..426
                     /label=ITR_misc_feature
                     /note="note=""ITR"" - 259..426"
                     /note="color: #80ffff; direction: RIGHT"
     promoter        451..1385
                     /label=CBA promoter_promoter
                     /note="note=""CBA promoter"" - 451..1385"
                     /note="color: #c0c0c0; direction: RIGHT"
     misc_feature    1256..1278
                     /label=pCAG-N 0.03
                     /note="color: #9b43df; direction: RIGHT"
     misc_feature    1401..1538
                     /label=Signal peptide
                     /note="color: #529cff; direction: RIGHT"
     misc_feature    1551..1775
                     /label=X513OpuB 30-106
                     /note="note=""X513OpuB 30-106"" - 805..1029"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    1776..1787
                     /label=Linker (N)
                     /note="color: #0378ff; direction: RIGHT"
     misc_feature    1788..2510
                     /label=cpsfGFP
                     /note="color: #5fff45; direction: RIGHT"
     misc_feature    2511..2519
                     /label=Linker (C)
                     /note="color: #0378ff; direction: RIGHT"
     misc_feature    2520..3113
                     /label=X513OpuB 107-305
                     /note="note=""X513OpuB 107-305"" - 1774..2367"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    3120..3332
                     /label=neuroligin extracellular
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    3333..3392
                     /label=transmembrane predicted
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             complement(3387..3398)
                     /label=FactorXa site
                     /note="color: #c0c0c0; direction: LEFT"
     misc_feature    3393..3773
                     /label=neuroligin intracellular
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    3774..3791
                     /label=Linker stop
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    3804..4392
                     /label=WPRE_misc_feature
                     /note="note=""WPRE"" - 2764..3352"
                     /note="color: #c0c0c0; direction: RIGHT"
     misc_feature    3940..3959
                     /label=pCAG-C 0.02
                     /note="color: #9b43df; direction: RIGHT"
     CDS             complement(4275..4286)
                     /label=Factor Xa site_Factor Xa recognition and cleava...
                     /note="codon_start=1 - note=""Factor Xa recognition and 
                     cleavage site"" - note=""Factor Xa site"" - 
                     translation=""IEGR"" - complement(3235..3246)"
                     /note="color: #c0c0c0; direction: LEFT"
     misc_feature    4420..4652
                     /label=SV40_misc_feature
                     /note="note=""SV40"" - 3380..3612"
                     /note="color: #c0c0c0; direction: RIGHT"
     misc_feature    complement(4680..4847)
                     /label=ITR_misc_feature
                     /note="note=""ITR"" - 3640..3807"
                     /note="color: #6ef8ff; direction: LEFT"
     rep_origin      5024..5479
                     /direction=RIGHT
                     /label=f1 bacteriophage origin of replication; arrow _...
                     /note="direction=RIGHT - note=""RIGHT"" - note=""f1 
                     bacteriophage origin of replication; arrow - indicates 
                     direction of (+) strand synthesis"" - 3984..4439"
                     /note="color: #c0c0c0"
     promoter        5505..5609
                     /label=AmpR promoter_bla_promoter
                     /note="note=""bla"" - note=""AmpR promoter"" - 4465..4569"
                     /note="color: #c0c0c0; direction: RIGHT"
     CDS             5610..6470
                     /label=confers resistance to ampicillin, carbenicillin...
                     /note="codon_start=1 - note=""bla"" - note=""confers 
                     resistance to ampicillin, carbenicillin, and - related 
                     antibiotics"" - 
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                      - 
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                      - PVTEKHLTDGMTVRELCSA"
                     /note="color: #c0c0c0; direction: RIGHT"
     rep_origin      6641..7229
                     /direction=RIGHT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o...
                     /note="direction=RIGHT - note=""RIGHT"" - 
                     note=""high-copy-number ColE1/pMB1/pBR322/pUC origin of - 
                     replication"" - 5601..6189"
                     /note="color: #c0c0c0"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      721 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      781 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      841 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      901 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      961 aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg
     1021 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1081 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1141 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1201 gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     1261 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     1321 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     1381 gaattggatc tgccgccacc atggcacttc ctagatgtat gtggcctaat tatgtatggc
     1441 gagctatgat ggcctgcgtg gtgcaccgag ggagtggcgc cccacttaca ctctgcctgt
     1501 tgggttgcct cctccagact ttccacgttc tttcacagaa gctggaattc gcgaacgaca
     1561 ccgtagttgt gggctcgatt aaccatacag aacagattat cgtcgcaaac atgttggcag
     1621 agatgattga ggcgcataca gaccttaagg tggttcgcaa actgaacctt ggcggggtta
     1681 acgttaactt tgaagccatt aaacgcggag gtgcgaataa tggtattgac atttacctgg
     1741 agtacgttgg gtatggtctt gtggatattc tggggtaccc ggagccgaac gtctatatca
     1801 tcgccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgctac aacgtggagg
     1861 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccctg
     1921 tgctgctgcc tgacaaccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
     1981 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2041 tggacgagct gtacaagggc ggtaccggag ggagcatgag caagggcgag gagctgttca
     2101 ccggggtggt gcccatcctg gtcgagctgg acggcggcgt aaacggccac aagttcagcg
     2161 tgcgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca
     2221 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2281 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2341 ccgaaggcta cgtccaggag cgcaccatca gcttcaagga cgacggcacc tacaagaccc
     2401 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2461 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttt ccgccgcccg
     2521 gtgctactga tccagaaggt gcatacgaaa ccgtgaagaa ggagtacaaa cgtaaatgga
     2581 atattgtatg gctcaaacca ctgggattca acgcttcgta tgtgcttgcg gttaaagacg
     2641 aactggcgaa acagtataac cttaaaacct tcagtgactt agcgaaaatc tcggataagc
     2701 tgattctggg tgcaaacatg atgtttttag aaaatcccga tggttaccca ggcctgcaaa
     2761 aactgtacaa tttcaaattc aagcacacca aaagcatgga cgctggtatt ccgtataccg
     2821 ccattgataa taacgaagtt caggtaattg atgccactgc cactgatggc ttgctggtga
     2881 gccacaaatt aaaaattctg gaggatgata aagcgttctt cccgccgtat tatgctgccc
     2941 ccatcatccg tcaggatgtc ttagataagc atcctgaact gaaggacgtg ctgaacaaac
     3001 tcgcgaatca aatttcactg gaagaaatgc agaaactgaa ttacaagcgt gacggtgagg
     3061 gtcaggaccc agcgaaagta gctaaggagt ttttgaaaga gaaaggttta attggatcca
     3121 cgctcgagct ggtgccccac ttgcataacc tcaatgacat cagccaatac acaagtacaa
     3181 ccaccaaggt cccaagcact gacatcaccc ttcgaccaac tcggaaaaac tcaacccctg
     3241 tgaccagcgc atttccaacc gctaagcaag acgaccccaa acaacagcct agtccattta
     3301 gcgtagacca aagggactac agcacagagc tgtccgtgac catagctgta ggagcttccc
     3361 tgcttttcct gaacattctg gctttcgcgg ccctctatta caagaaggac aaaaggcgcc
     3421 acgacgtaca taggagatgt agtccgcaac gaactacaac caatgatctt acgcacgcac
     3481 ctgaggagga gatcatgagt cttcagatga aacacacaga cctggaccac gagtgtgaat
     3541 ccatccatcc ccatgaggtg gtgctgagga cagcatgccc tccggactat accctggcaa
     3601 tgcgccgaag cccagacgac attcccctga tgacacccaa taccataacc atgattccca
     3661 acactattcc aggaatccag cctctccaca ctttcaacac attcactggt ggacagaata
     3721 atactctccc tcatccgcat ccacaccccc actcccacag cacaacacgg gtgggtggca
     3781 gcggtggcta aaagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     3841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4201 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4321 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4381 ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat
     4441 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4501 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4561 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
     4621 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac
     4681 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
     4741 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4801 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct
     4861 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5041 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5101 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5161 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5221 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5281 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5341 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5401 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5461 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5521 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5581 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5641 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5701 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5761 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5821 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5881 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5941 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6001 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6061 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6121 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6181 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6241 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6301 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6361 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6421 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6481 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6541 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6601 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6661 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6721 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6781 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6841 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6901 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6961 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7021 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7081 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7141 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7201 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7261 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7321 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7381 gcagcgagtc agtgagcgag gaagcggaag
//
