LOCUS       Exported                6907 bp ds-DNA     circular SYN 31-MAY-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 6, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
COMMENT     Ligation of : pAAV-CAG-FLEX-YMAT-PDGFR-3.xdna [5064 nt] : 
            (SpeI[2952] / XhoI[1113]) to PCR(pMinDis-L310E10-PDGFR-EnhExp.xdna) 
            [1883 nt] : (XhoI[1891] / SpeI[4])
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..249
                     /label=CMV Enhancer
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    251..528
                     /label=Chicken B-actin Promoter
                     /note="color: #92abc4; direction: RIGHT"
     primer_bind     808..830
                     /label=CAG-F
                     /note="color: #9b43df; direction: RIGHT"
     misc_feature    892..911
                     /label=pCAG_F_primer
                     /note="label=""pCAG_F_primer"" - ApEinfo_revcolor=#f58a5e -
                     ApEinfo_fwdcolor=#f58a5e - 3542..3561"
                     /note="color: #9b43df; direction: RIGHT"
     misc_feature    946..979
                     /label=lox2722
                     /note="label=""loxP"" - ApEinfo_revcolor=#9eafd2 - 
                     ApEinfo_fwdcolor=#9eafd2 - 3596..3629"
                     /note="color: #1da0ff; direction: RIGHT"
     misc_feature    1042..1075
                     /label=LoxP
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    complement(1119..1205)
                     /label=PDGFR transmembrane helix
                     /note="note=""PDGFR transmembrane helix"" - 915..983"
                     /note="color: #529cff; direction: LEFT"
     CDS             complement(1272..1301)
                     /label=Myc_Myc (human c-Myc oncogene) epitope tag_CDS
                     /note="codon_start=1 - note=""Myc (human c-Myc oncogene) 
                     epitope tag"" - note=""Myc"" - translation=""EQKLISEEDL"" -
                     819..848"
                     /note="color: #8080ff; direction: LEFT"
     misc_feature    complement(1311..1907)
                     /label=X513OpuB 107-305
                     /note="note=""X513OpuB 107-305"" - 1774..2367"
                     /note="color: #dc143c; direction: LEFT"
     misc_feature    complement(1908..1916)
                     /label=Linker (C)
                     /note="color: #0378ff; direction: LEFT"
     misc_feature    complement(1917..2639)
                     /label=cpsfGFP
                     /note="color: #5fff45; direction: LEFT"
     misc_feature    complement(2640..2651)
                     /label=Linker (N)
                     /note="color: #0378ff; direction: LEFT"
     misc_feature    complement(2652..2876)
                     /label=X513OpuB 30-106
                     /note="note=""X513OpuB 30-106"" - 805..1029"
                     /note="color: #dc143c; direction: LEFT"
     misc_feature    complement(2877..2882)
                     /label=MCS_misc_feature
                     /note="note=""MCS"" - 799..804"
                     /note="color: #b5978d; direction: LEFT"
     CDS             complement(2883..2945)
                     /label=Ig-kappa leader_leader sequence from mouse_CDS
                     /note="codon_start=1 - note=""leader sequence from mouse"" 
                     - note=""Ig-kappa leader"" - 
                     translation=""METDTLLLWVLLLWVPGSTGD"" - 737..799"
                     /note="color: #f08080; direction: LEFT"
     CDS             complement(2943..2945)
                     /codon_start=1
                     /label=Start
                     /note="color: #993366"
                     /translation="M"
     misc_feature    2980..3013
                     /label=lox2722
                     /note="color: #1792ff; direction: RIGHT"
     misc_recomb     3084..3117
                     /label=LoxP
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    3161..3748
                     /label=WPRE
                     /note="label=""WPRE"" - ApEinfo_revcolor=#d59687 - 
                     ApEinfo_fwdcolor=#d59687 - 5531..6118"
                     /note="color: #a7a8a8; direction: RIGHT"
     primer_bind     3295..3314
                     /label=pCAG-C
                     /note="color: #9b43df; direction: RIGHT"
     CDS             complement(3630..3641)
                     /label=FactorXa site
                     /note="color: #008000; direction: LEFT"
     terminator      complement(3787..3906)
                     /label=SV40_PA_terminator
                     /note="label=""SV40_PA_terminator"" - 
                     ApEinfo_revcolor=#f58a5e - ApEinfo_fwdcolor=#f58a5e - 
                     complement(6157..6276)"
                     /note="color: #a7a8a8; direction: LEFT"
     misc_feature    complement(3801..3820)
                     /label=EBV_rev_primer
                     /note="label=""EBV_rev_primer"" - ApEinfo_revcolor=#84b0dc 
                     - ApEinfo_fwdcolor=#84b0dc - complement(6171..6190)"
                     /note="color: #9b43df; direction: LEFT"
     misc_feature    complement(4035..4202)
                     /label=R-ITR
                     /note="color: #80ffff; direction: LEFT"
     rep_origin      complement(4421..5049)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #a7a8a8"
     gene            complement(5198..6058)
                     /label=Ampicillin
                     /note="label=""Ampicillin"" - ApEinfo_revcolor=#d6b295 - 
                     ApEinfo_fwdcolor=#d6b295 - complement(941..1801)"
                     /note="color: #a7a8a8; direction: LEFT"
     promoter        complement(6059..6163)
                     /label=AmpR promoter_bla_promoter
                     /note="gene=""bla"" - note=""AmpR promoter"" - 937..1041"
                     /note="color: #a7a8a8; direction: LEFT"
     rep_origin      complement(6189..6644)
                     /direction=LEFT
                     /label=f1 bacteriophage origin of replication; arrow _...
                     /note="direction=RIGHT - note=""f1 ori"" - note=""f1 
                     bacteriophage origin of replication; arrow - indicates 
                     direction of (+) strand synthesis"" - 456..911"
                     /note="color: #a7a8a8"
     rep_origin      6199..6639
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #a7a8a8"
     CDS             complement(6646..6714)
                     /label=LacZ alpha
                     /note="color: #a7a8a8; direction: LEFT"
     misc_feature    6716..6883
                     /label=L-ITR
                     /note="color: #80ffff; direction: RIGHT"
ORIGIN
        1 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
       61 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      121 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      181 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      241 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
      301 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
      361 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
      421 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
      481 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
      541 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      601 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      661 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      721 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      781 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      841 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
      901 tggttattgt gctgtctcat cattttggca aagaattgga tccccataac ttcgtataaa
      961 gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc gacccgaatt
     1021 gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa gttatactag
     1081 ggatcccctt acttgtacag agctcggcgc gcctcgagct aacgtggctt cttctgccaa
     1141 agcatgatga ggatgataag ggagatgatg gtgagcacca ccagggccag gatggctgag
     1201 atcaccacca ccttaaaggg caaggagtgt ggcaccacga tgacctcctg cgtgtcctgg
     1261 cccacagcat tcagatcctc ttctgagatg agtttttgtt cgtcgacctg cagaattaaa
     1321 cctttctctt tcaaaaactc cttagctact ttcgctgggt cctgaccctc accgtcacgc
     1381 ttgtaattca gtttctgcat ttcttccagt gaaatttgat tcgcgagttt gttcagcacg
     1441 tccttcagtt caggatgctt atctaagaca tcctgacgga tgatgggggc agcataatac
     1501 ggcgggaaga acgctttatc atcctccaga atttttaatt tgtggctcac cagcaagcca
     1561 tcagtggcag tggcatcaat tacctgaact tcgttattat caatggcggt atacggaata
     1621 ccagcgtcca tgcttttggt gtgcttgaat ttgaaattgt acagtttttg caggcctggg
     1681 taaccatcgg gattttctaa aaacatcatg tttgcaccca gaatcagctt atccgagatt
     1741 ttcgctaagt cactgaaggt tttaaggtta tactgtttcg ccagttcgtc tttaaccgca
     1801 agcacatacg aagcgttgaa tcccagtggt ttgagccata caatattcca tttacgtttg
     1861 tactccttct tcacggtttc gtatgcacct tctggatcag tagcaccggg cggcggaaag
     1921 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     1981 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtaggtg
     2041 ccgtcgtcct tgaagctgat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     2101 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     2161 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     2221 agggtcagct tgccgttggt ggcatcgccc tcgccctcgc cgcgcacgct gaacttgtgg
     2281 ccgtttacgc cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     2341 cccttgctca tgctccctcc ggtaccgccc ttgtacagct cgtccatgcc gagagtgatc
     2401 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     2461 ctcagcacgg actgggtgct caggtagtgg ttgtcaggca gcagcacagg gccgtcgccg
     2521 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc cacgttgtag
     2581 cggatcttga agttcgcctt gatgccgttc ttctgcttgt cggcgatgat atagacgttc
     2641 ggctccgggt accccagaat atccacaaga ccatacccaa cgtactccag gtaaatgtca
     2701 ataccattat tcgcacctcc gcgtttaatg gcttcaaagt taacgttaac cccgccaagg
     2761 ttcagtttgc gaaccacctt aaggtctgta tgcgcctcaa tcatctctgc caacatgttt
     2821 gcgacgataa tctgttctgt atggttaatc gagcccacaa ctacggtgtc gttcgcagat
     2881 ctgtcaccag tggaacctgg aacccagagc agcagtaccc atagcaggag tgtgtctgtc
     2941 tccatggtgg cactagtgct agcggtaccg aattctagta taacttcgta taggatactt
     3001 tatacgaagt tatcattggg attcttccta ttttgatcca agcatcacca tcgaccctct
     3061 agtccagatc tcaccatcga cccataactt cgtatagcat acattatacg aagttatgtc
     3121 cctcgaagag gttcgaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     3181 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3241 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3301 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3361 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3421 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3481 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3541 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3601 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3661 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3721 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acccgggcgg ccgcttcgag
     3781 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3841 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3901 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
     3961 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc
     4021 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     4081 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4141 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4201 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4261 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4321 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4381 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4441 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4501 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4561 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4621 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4681 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4741 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4801 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4861 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4921 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4981 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5041 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5101 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5161 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5221 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5281 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5341 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5401 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5461 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5521 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5581 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5641 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5701 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5761 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5821 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5881 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5941 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6001 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6061 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6121 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6181 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6241 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6301 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6361 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6421 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6481 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6541 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6601 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     6661 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctgcg
     6721 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
     6781 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
     6841 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
     6901 gatcgta
//
