LOCUS       Exported                1595 bp ds-DNA     linear   UNA 07-JUN-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2019 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        complement(21..99)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        408..426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    427..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             466..488
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
     CDS             507..524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             534..554
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /note="color: #cc99b2
                     Cleavage site after base 551"
                     /translation="ENLYFQG"
     misc_feature    556..561
                     /label=NcoI
                     /note="color: #ff0000"
     misc_feature    561..1004
                     /label=GRAB (V34-N181)
                     /note="color: #ff9900"
     misc_feature    1008..1013
                     /label=XhoI
                     /note="color: #ff0000"
     CDS             1014..1031
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      1098..1145
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff"
     rep_origin      1182..1565
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 tataacgtta ctggtttcac attcaccacc ctgaattgac tctttttccg ggcgctatca
       61 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
      121 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
      181 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
      241 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
      301 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
      361 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
      421 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      481 gaaggagata tcatatgggc agcagccatc atcatcatca tcacagcagc ggcgaaaacc
      541 tttacttcca gggcgccatg gttgatagcc cgattgaaca gcctcgtatt attccgaatg
      601 gtggcaccct gaccaatctg ctgggtaatg caccggaaaa actggcactg cgtaatgaag
      661 aacgtgcaat tgatgaactg aaaaaacagg ccatcgaaga taaagaagca accaccgcaa
      721 ttgaagcagc aagcagtgat gcactggaag cactggcaga tcagaccgat gcactgcaga
      781 gcgaagaagc agcagttgtt aaagcagata atgcagcatc agacgccctg gaagccctgg
      841 ccgaccaaac agacgcgctg cagtcagaag aagccgaagt tgtacagagt gataatgccg
      901 caagtgatgc atgggaaaaa gcagcaaccc cgattgcact ggatgtgaaa aaaaccaaag
      961 ataccaaacc ggtggtgaaa aaagaagaac gtcagaacgt taattaactc gagcaccacc
     1021 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     1081 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     1141 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
     1201 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1261 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1321 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1381 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1441 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1501 gaacaacact caaccctatc tcggtctatt cttttgaatt taaangggat tttggccgat
     1561 ttcggccnta ttggttaaaa aatggagctg aattt
//
