LOCUS       pMJA103=Hu\LysozymeD67H        8398 bp    DNA     circular      9-SEP-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|281801258|
COMMENT     VNTDBDATE|600782988|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMJA103=Hu LysozymeD67H|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     promoter        1..948
                     /vntifkey="29"
                     /label=5'-AOX1\promoter
     CDS             949..1215
                     /vntifkey="4"
                     /label=secretion\seq-\a-factor
     misc_feature    1628..1961
                     /vntifkey="21"
                     /label=3'\AOX1\transc.\term.
     CDS             complement(2355..4889)
                     /vntifkey="4"
                     /label=HIS4\ORF
     CDS             5245..6001
                     /vntifkey="4"
                     /label=3'\AOX1\fragment
     rep_origin      complement(6409..7083)
                     /vntifkey="33"
                     /label=ColE1\origin
     CDS             complement(7228..8088)
                     /vntifkey="4"
                     /label=Ampicillin\resistance\gene
     CDS             1204..1596
                     /vntifkey="4"
                     /label=Human\lysozyme
BASE COUNT     2237 a      1976 c      1905 g      2280 t 
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 
     1201 agaaaggttt tcgaaagatg tgagctcgct agaaccttga agagattggg tatggacggg 
     1261 taccgtggta tctctttggc taactggatg tgtttggcta agtgggaatc tggttacaac 
     1321 actcgagcta ccaactacaa cgctggtgac aggtcgaccg actacggtat cttccaaatt 
     1381 aattctagat actggtgtaa ccacggtaag accccaggcg ccgttaacgc ttgtcacttg 
     1441 tcttgttctg ctttgctgca ggacaacatc gctgacgctg ttgcttgtgc taaacgcgtt 
     1501 gttagagacc cacaaggtat cagagcttgg gttgcttgga gaaatcgatg tcaaaacaga 
     1561 gacgtcagac aatacgttca aggttgtggt gtttaggaat tccctagggc ggccgcgaat 
     1621 taattcgcct tagacatgac tgttcctcag ttcaagttgg gcacttacga gaagaccggt 
     1681 cttgctagat tctaatcaag aggatgtcag aatgccattt gcctgagaga tgcaggcttc 
     1741 atttttgata cttttttatt tgtaacctat atagtatagg attttttttg tcattttgtt 
     1801 tcttctcgta cgagcttgct cctgatcagc ctatctcgca gctgatgaat atcttgtggt 
     1861 aggggtttgg gaaaatcatt cgagtttgat gtttttcttg gtatttccca ctcctcttca 
     1921 gagtacagaa gattaagtga gaagttcgtt tgtgcaagct tatcgataag ctttaatgcg 
     1981 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg 
     2041 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc 
     2101 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg 
     2161 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac 
     2221 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg 
     2281 actacgcgat catggcgacc acacccgtcc tgtggatcta tcgaatctaa atgtaagtta 
     2341 aaatctctaa ataattaaat aagtcccagt ttctccatac gaaccttaac agcattgcgg 
     2401 tgagcatcta gaccttcaac agcagccaga tccatcactg cttggccaat atgtttcagt 
     2461 ccctcaggag ttacgtcttg tgaagtgatg aacttctgga aggttgcagt gttaactccg 
     2521 ctgtattgac gggcatatcc gtacgttggc aaagtgtggt tggtaccgga ggagtaatct 
     2581 ccacaactct ctggagagta ggcaccaaca aacacagatc cagcgtgttg tacttgatca 
     2641 acataagaag aagcattctc gatttgcagg atcaagtgtt caggagcgta ctgattggac 
     2701 atttccaaag cctgctcgta ggttgcaacc gatagggttg tagagtgtgc aatacacttg 
     2761 cgtacaattt caacccttgg caactgcaca gcttggttgt gaacagcatc ttcaattctg 
     2821 gcaagctcct tgtctgtcat atcgacagcc aacagaatca cctgggaatc aataccatgt 
     2881 tcagcttgag acagaaggtc tgaggcaacg aaatctggat cagcgtattt atcagcaata 
     2941 actagaactt cagaaggccc agcaggcatg tcaatactac acagggctga tgtgtcattt 
     3001 tgaaccatca tcttggcagc agtaacgaac tggtttcctg gaccaaatat tttgtcacac 
     3061 ttaggaacag tttctgttcc gtaagccata gcagctactg cctgggcgcc tcctgctagc 
     3121 acgatacact tagcaccaac cttgtgggca acgtagatga cttctggggt aagggtacca 
     3181 tccttcttag gtggagatgc aaaaacaatt tctttgcaac cagcaacttt ggcaggaaca 
     3241 cccagcatca gggaagtgga aggcagaatt gcggttccac caggaatata gaggccaact 
     3301 ttctcaatag gtcttgcaaa acgagagcag actacaccag ggcaagtctc aacttgcaac 
     3361 gtctccgtta gttgagcttc atggaatttc ctgacgttat ctatagagag atcaatggct 
     3421 ctcttaacgt tatctggcaa ttgcataagt tcctctggga aaggagcttc taacacaggt 
     3481 gtcttcaaag cgactccatc aaacttggca gttagttcta aaagggcttt gtcaccattt 
     3541 tgacgaacat tgtcgacaat tggtttgact aattccataa tctgttccgt tttctggata 
     3601 ggacgacgaa gggcatcttc aatttcttgt gaggaggcct tagaaacgtc aattttgcac 
     3661 aattcaatac gaccttcaga agggacttct ttaggtttgg attcttcttt aggttgttcc 
     3721 ttggtgtatc ctggcttggc atctcctttc cttctagtga cctttaggga cttcatatcc 
     3781 aggtttctct ccacctcgtc caacgtcaca ccgtacttgg cacatctaac taatgcaaaa 
     3841 taaaataagt cagcacattc ccaggctata tcttccttgg atttagcttc tgcaagttca 
     3901 tcagcttcct ccctaatttt agcgttcaac aaaacttcgt cgtcaaataa ccgtttggta 
     3961 taagaacctt ctggagcatt gctcttacga tcccacaagg tggcttccat ggctctaaga 
     4021 ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac agaaaccaac acctgtttgt 
     4081 tcaaccacaa atttcaagca gtctccatca caatccaatt cgatacccag caacttttga 
     4141 gttgctccag atgtagcacc tttataccac aaaccgtgac gacgagattg gtagactcca 
     4201 gtttgtgtcc ttatagcctc cggaatagac tttttggacg agtacaccag gcccaacgag 
     4261 taattagaag agtcagccac caaagtagtg aatagaccat cggggcggtc agtagtcaaa 
     4321 gacgccaaca aaatttcact gacagggaac tttttgacat cttcagaaag ttcgtattca 
     4381 gtagtcaatt gccgagcatc aataatgggg attataccag aagcaacagt ggaagtcaca 
     4441 tctaccaact ttgcggtctc agaaaaagca taaacagttc tactaccgcc attagtgaaa 
     4501 cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga tactagcatt agcgggcaag 
     4561 gatgcaactt tatcaaccag ggtcctatag ataaccctag cgcctgggat catcctttgg 
     4621 acaactcttt ctgccaaatc taggtccaaa atcacttcat tgataccatt attgtacaac 
     4681 ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg taacggatga ctcaacttgc 
     4741 acattaactt gaagctcagt cgattgagtg aacttgatca ggttgtgcag ctggtcagca 
     4801 gcatagggaa acacggcttt tcctaccaaa ctcaaggaat tatcaaactc tgcaacactt 
     4861 gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg gacagtgagt gtagtcttga 
     4921 gaaattctga agccgtattt ttattatcag tgagtcagtc atcaggagat cctctacgcc 
     4981 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 
     5041 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 
     5101 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 
     5161 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 
     5221 caggagtcgc ataagggaga gcgtcgagta tctatgattg gaagtatggg aatggtgata 
     5281 cccgcattct tcagtgtctt gaggtctcct atcagattat gcccaactaa agcaaccgga 
     5341 ggaggagatt tcatggtaaa tttctctgac ttttggtcat cagtagactc gaactgtgag 
     5401 actatctcgg ttatgacagc agaaatgtcc ttcttggaga cagtaaatga agtcccacca 
     5461 ataaagaaat ccttgttatc aggaacaaac ttcttgtttc gaactttttc ggtgccttga 
     5521 actataaaat gtagagtgga tatgtcgggt aggaatggag cgggcaaatg cttaccttct 
     5581 ggaccttcaa gaggtatgta gggtttgtag atactgatgc caacttcagt gacaacgttg 
     5641 ctatttcgtt caaaccattc cgaatccaga gaaatcaaag ttgtttgtct actattgatc 
     5701 caagccagtg cggtcttgaa actgacaata gtgtgctcgt gttttgaggt catctttgta 
     5761 tgaataaatc tagtctttga tctaaataat cttgacgagc caaggcgata aatacccaaa 
     5821 tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct gcctgtatta aaccccaaat 
     5881 cagctcgtag tctgatcctc atcaacttga ggggcactat cttgttttag agaaatttgc 
     5941 ggagatgcga tatcgagaaa aaggtacgct gattttaaac gtgaaattta tctcaagatc 
     6001 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 
     6061 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 
     6121 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 
     6181 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 
     6241 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 
     6301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     6361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     6421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     6481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     6541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     6601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     6661 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     6721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     6781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     6841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     6901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     6961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     7021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     7081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     7141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     7201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     7261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     7321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     7381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     7441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     7501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc 
     7561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     7621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     7681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     7741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     7801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc 
     7861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     7921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     7981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     8041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     8101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     8161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
     8221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 
     8281 ctttcgtctt caagaattaa ttctcatgtt tgacagctta tcatcgataa gctgactcat 
     8341 gttggtattg tgaaatagac gcagatcggg aacactgaaa aataacagtt attattcg 
//
