LOCUS       pMJA153=\pIC9+BN\intronless\-S96A-S1489A\c1485t        8349 bp    DNA     circular      3-JUN-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|377858487|
COMMENT     VNTDBDATE|-12842682|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMJA153= pIC9+BN intronless -S96A-S1489A c1485t|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     promoter        1..948
                     /vntifkey="29"
                     /label=5'-AOX1\promoter
     CDS             949..1215
                     /vntifkey="4"
                     /label=secretion\seq-\a-factor
     misc_feature    1579..1912
                     /vntifkey="21"
                     /label=3'\AOX1\transc.\term.
     CDS             complement(join(2306..4840))
                     /vntifkey="4"
                     /label=HIS4\ORF
     CDS             5196..5952
                     /vntifkey="4"
                     /label=3'\AOX1\fragment
     rep_origin      complement(join(6360..7034))
                     /vntifkey="33"
                     /label=ColE1\origin
     CDS             complement(join(7179..8039))
                     /vntifkey="4"
                     /label=Ampicillin\resistance\gene
     exon            1204..1548
                     /vntifkey="61"
                     /label=Ber\e\1
BASE COUNT     2230 a      1970 c      1924 g      2225 t 
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 
     1201 agagaggctg aagctcagga ggagtgtcgc gagcagatgc agagacagca gatgctcagc 
     1261 cactgccgga tgtatatgag acagcagatg gaggagagca cgtaccagac catgcccagg 
     1321 cggggaatgg agccgcatat gagcgagtgc tgcgagcagc tggaggggat ggacgagagc 
     1381 tgcagatgcg aaggcttaag gatgatgatg aggatgatgc aacagaagga gatgcaaccc 
     1441 cgaggggagc agatgcgaag gatgatgagg ctggccgaga atattcctgc ccgctgcaac 
     1501 ctcagtccca tgagatgccc catgggtggc tccattgccg ggttctgaga attcctaggg 
     1561 cggccgcgaa ttaattcgcc ttagacatga ctgttcctca gttcaagttg ggcacttacg 
     1621 agaagaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt tgcctgagag 
     1681 atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag gatttttttt 
     1741 gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc agctgatgaa 
     1801 tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt ggtatttccc 
     1861 actcctcttc agagtacaga agattaagtg agaagttcgt ttgtgcaagc ttatcgataa 
     1921 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa 
     1981 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg 
     2041 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc 
     2101 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt 
     2161 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg 
     2221 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatct atcgaatcta 
     2281 aatgtaagtt aaaatctcta aataattaaa taagtcccag tttctccata cgaaccttaa 
     2341 cagcattgcg gtgagcatct agaccttcaa cagcagccag atccatcact gcttggccaa 
     2401 tatgtttcag tccctcagga gttacgtctt gtgaagtgat gaacttctgg aaggttgcag 
     2461 tgttaactcc gctgtattga cgggcatatc cgtacgttgg caaagtgtgg ttggtaccgg 
     2521 aggagtaatc tccacaactc tctggagagt aggcaccaac aaacacagat ccagcgtgtt 
     2581 gtacttgatc aacataagaa gaagcattct cgatttgcag gatcaagtgt tcaggagcgt 
     2641 actgattgga catttccaaa gcctgctcgt aggttgcaac cgatagggtt gtagagtgtg 
     2701 caatacactt gcgtacaatt tcaacccttg gcaactgcac agcttggttg tgaacagcat 
     2761 cttcaattct ggcaagctcc ttgtctgtca tatcgacagc caacagaatc acctgggaat 
     2821 caataccatg ttcagcttga gacagaaggt ctgaggcaac gaaatctgga tcagcgtatt 
     2881 tatcagcaat aactagaact tcagaaggcc cagcaggcat gtcaatacta cacagggctg 
     2941 atgtgtcatt ttgaaccatc atcttggcag cagtaacgaa ctggtttcct ggaccaaata 
     3001 ttttgtcaca cttaggaaca gtttctgttc cgtaagccat agcagctact gcctgggcgc 
     3061 ctcctgctag cacgatacac ttagcaccaa ccttgtgggc aacgtagatg acttctgggg 
     3121 taagggtacc atccttctta ggtggagatg caaaaacaat ttctttgcaa ccagcaactt 
     3181 tggcaggaac acccagcatc agggaagtgg aaggcagaat tgcggttcca ccaggaatat 
     3241 agaggccaac tttctcaata ggtcttgcaa aacgagagca gactacacca gggcaagtct 
     3301 caacttgcaa cgtctccgtt agttgagctt catggaattt cctgacgtta tctatagaga 
     3361 gatcaatggc tctcttaacg ttatctggca attgcataag ttcctctggg aaaggagctt 
     3421 ctaacacagg tgtcttcaaa gcgactccat caaacttggc agttagttct aaaagggctt 
     3481 tgtcaccatt ttgacgaaca ttgtcgacaa ttggtttgac taattccata atctgttccg 
     3541 ttttctggat aggacgacga agggcatctt caatttcttg tgaggaggcc ttagaaacgt 
     3601 caattttgca caattcaata cgaccttcag aagggacttc tttaggtttg gattcttctt 
     3661 taggttgttc cttggtgtat cctggcttgg catctccttt ccttctagtg acctttaggg 
     3721 acttcatatc caggtttctc tccacctcgt ccaacgtcac accgtacttg gcacatctaa 
     3781 ctaatgcaaa ataaaataag tcagcacatt cccaggctat atcttccttg gatttagctt 
     3841 ctgcaagttc atcagcttcc tccctaattt tagcgttcaa caaaacttcg tcgtcaaata 
     3901 accgtttggt ataagaacct tctggagcat tgctcttacg atcccacaag gtggcttcca 
     3961 tggctctaag accctttgat tggccaaaac aggaagtgcg ttccaagtga cagaaaccaa 
     4021 cacctgtttg ttcaaccaca aatttcaagc agtctccatc acaatccaat tcgataccca 
     4081 gcaacttttg agttgctcca gatgtagcac ctttatacca caaaccgtga cgacgagatt 
     4141 ggtagactcc agtttgtgtc cttatagcct ccggaataga ctttttggac gagtacacca 
     4201 ggcccaacga gtaattagaa gagtcagcca ccaaagtagt gaatagacca tcggggcggt 
     4261 cagtagtcaa agacgccaac aaaatttcac tgacagggaa ctttttgaca tcttcagaaa 
     4321 gttcgtattc agtagtcaat tgccgagcat caataatggg gattatacca gaagcaacag 
     4381 tggaagtcac atctaccaac tttgcggtct cagaaaaagc ataaacagtt ctactaccgc 
     4441 cattagtgaa acttttcaaa tcgcccagtg gagaagaaaa aggcacagcg atactagcat 
     4501 tagcgggcaa ggatgcaact ttatcaacca gggtcctata gataacccta gcgcctggga 
     4561 tcatcctttg gacaactctt tctgccaaat ctaggtccaa aatcacttca ttgataccat 
     4621 tattgtacaa cttgagcaag ttgtcgatca gctcctcaaa ttggtcctct gtaacggatg 
     4681 actcaacttg cacattaact tgaagctcag tcgattgagt gaacttgatc aggttgtgca 
     4741 gctggtcagc agcataggga aacacggctt ttcctaccaa actcaaggaa ttatcaaact 
     4801 ctgcaacact tgcgtatgca ggtagcaagg gaaatgtcat acttgaagtc ggacagtgag 
     4861 tgtagtcttg agaaattctg aagccgtatt tttattatca gtgagtcagt catcaggaga 
     4921 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg 
     4981 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg 
     5041 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct 
     5101 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct 
     5161 gcttcctaat gcaggagtcg cataagggag agcgtcgagt atctatgatt ggaagtatgg 
     5221 gaatggtgat acccgcattc ttcagtgtct tgaggtctcc tatcagatta tgcccaacta 
     5281 aagcaaccgg aggaggagat ttcatggtaa atttctctga cttttggtca tcagtagact 
     5341 cgaactgtga gactatctcg gttatgacag cagaaatgtc cttcttggag acagtaaatg 
     5401 aagtcccacc aataaagaaa tccttgttat caggaacaaa cttcttgttt cgaacttttt 
     5461 cggtgccttg aactataaaa tgtagagtgg atatgtcggg taggaatgga gcgggcaaat 
     5521 gcttaccttc tggaccttca agaggtatgt agggtttgta gatactgatg ccaacttcag 
     5581 tgacaacgtt gctatttcgt tcaaaccatt ccgaatccag agaaatcaaa gttgtttgtc 
     5641 tactattgat ccaagccagt gcggtcttga aactgacaat agtgtgctcg tgttttgagg 
     5701 tcatctttgt atgaataaat ctagtctttg atctaaataa tcttgacgag ccaaggcgat 
     5761 aaatacccaa atctaaaact cttttaaaac gttaaaagga caagtatgtc tgcctgtatt 
     5821 aaaccccaaa tcagctcgta gtctgatcct catcaacttg aggggcacta tcttgtttta 
     5881 gagaaatttg cggagatgcg atatcgagaa aaaggtacgc tgattttaaa cgtgaaattt 
     5941 atctcaagat ctctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 
     6001 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 
     6061 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 
     6121 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 
     6181 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 
     6241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     6301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     6361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     6421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     6481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     6541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     6601 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     6661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     6721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     6781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     6841 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     6901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     6961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     7021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     7081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     7141 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     7201 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     7261 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     7321 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     7381 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     7441 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc 
     7501 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     7561 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     7621 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     7681 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     7741 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg 
     7801 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     7861 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     7921 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     7981 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     8041 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     8101 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     8161 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 
     8221 atcacgaggc cctttcgtct tcaagaatta attctcatgt ttgacagctt atcatcgata 
     8281 agctgactca tgttggtatt gtgaaataga cgcagatcgg gaacactgaa aaataacagt 
     8341 tattattcg 
//
