LOCUS       pMJA295=NCBIpMJA251+Treenut-1\Nut\Gamma7Delta7        7557 bp    DNA     circular SYN 28-MAR-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12746396|
COMMENT     VNTDBDATE|-12746396|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMJA295=NCBIpMJA251+Treenut-1 Nut Gamma7Delta7|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Invitrogen|
COMMENT     VNTOAUTHORTEL|(800) 955-6288|
COMMENT     VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTOAUTHORAD2|5791 Van Allen Way|
COMMENT     VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     source          1..7557
                     /invitrogen="157"
                     /vntifkey="98"
     CDS             complement(6561..7421)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      3570..3794
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(3564..3581)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     promoter        complement(7422..7520)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     promoter        4658..4725
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     rep_origin      3840..4268
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     rep_origin      complement(5743..6416)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        4273..4643
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    5230..5360
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     CDS             4726..5100
                     /invitrogen="1370000"
                     /vntifkey="4"
                     /label=Zeo(R)
     promoter        1553..2319
                     /vntifkey="29"
                     /label=Bidirectional\promoter
     terminator      complement(251..772)
                     /vntifkey="43"
                     /label=Rabbit\terminator
     terminator      3570..3794
                     /vntifkey="43"
                     /label=BGH\pA
     exon            2413..3532
                     /vntifkey="61"
                     /label=Delta\chain
     misc_feature    2413..3038
                     /vntifkey="21"
                     /label=TRDV\Treenut-1\Nut\7
     misc_feature    2461..2683
                     /vntifkey="21"
                     /label=intro
     misc_feature    complement(779..1481)
                     /vntifkey="21"
                     /label=TRGV4
BASE COUNT     1906 a      1801 c      1851 g      1999 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagtaagaga agagggacag ctatgactgg gagtagtcag gagaggagga 
      301 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca 
      361 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat 
      421 ggacagcagg gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc 
      481 agccagcata tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa 
      541 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt 
      601 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg 
      661 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg 
      721 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttgaattcct 
      781 taataaaccc aatgcctttt tcttattaat gcccaacttt gtgacccaag aaaggttggg 
      841 gttagatttt cttttccatt tgcaggaaga aaacccgttt cttttaaggc agacagcagg 
      901 tggatatggc caaaataggc ccccctcttg aggagccgga ggaggtacgt gtaatatgca 
      961 gaggtgtttg tgagctgcag cattagtgta tcatttgcat cttttgacca attgtctttg 
     1021 gggtccattg tgatgacatc atttgcatct tttgaataac tgtctttggg gtccactgtg 
     1081 gtgacatctg tctttattgg aggaaagata atttcttgat caattccgtt tttattattc 
     1141 tcatgtctga cgatacatct gtgttctttg tccagtgact cttctggcac cgttaaccag 
     1201 ctaaatttca tgtatgtgtc gttagtcttc atggtgttcc cctcctggga gcccagaatc 
     1261 gtgttgctct tcttttcttg ccaatgtatc ttaataatat ctgggaaaaa tttctcaaga 
     1321 agacaaaggt atgttccagc cttctggagt tttgtttcag caattgaagg aagaaaaata 
     1381 gtgggcttgg gggaaacatc tgcatcaagt tgtttatctg tgacaacaag tgttgttcca 
     1441 ctgccgagac gaaagagttt cttataatac gcgtctcaca tgccttcctc tctgaggcct 
     1501 tggaaggaaa gggaccagat gaagaggagc agaagatcct cttaaaactc aggccccact 
     1561 caacaaggaa gtgatcccta gcacagaaga cagtaggagg gaggatccct agcggatctg 
     1621 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat 
     1681 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca 
     1741 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg 
     1801 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa 
     1861 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca 
     1921 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg 
     1981 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt 
     2041 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt 
     2101 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg 
     2161 gctatgaact aatgaccccg taattgatta ctattaataa ctactcgagt atgtcgaggt 
     2221 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct 
     2281 ggaggtacca ccggtcatat gccgcgggag cccagatcct gtgtgtgtgt gtgtgtgctt 
     2341 gtgcacatct gttctctgat tggctgactg tacttcctga gagtccctgt ttaatgttac 
     2401 aagaaacagc tgatgctgtt ctccagcctg ctgtgtgtat ttgtggcctt cagctactct 
     2461 ggtatggatg gttccaggtg tgagtaaaaa tgttaaggat ggaaggattc caaacaggca 
     2521 tatggtattt ctcatggact tgaggaggaa aggagaaatt gtaaaaaagc gagcatatta 
     2581 tgattttagt ttggtttgtc tctgggtcct aaattagtct agctcttacg atattttatt 
     2641 cactatatca aaggatcagc tctgttttct gatttttccc acaggatcaa gtgtggccca 
     2701 gaaggttact caagcccagt catcagtatc catgccagtg aggaaagcag tcaccctgaa 
     2761 ctgcctgtat gaaacaagtt ggtggtcata ttatattttt tggtacaagc agcctcctag 
     2821 caaagagatg attttcctta ttcgccaggg ttctgatgaa cagaatgcaa aaagtggtcg 
     2881 ctattctgtc aacttcaaga aagcagcgaa atccgtcgcc ttaaccattt cagccttaca 
     2941 gctagaagat tcagcaaagt acttttgtgc tcttggggtc tatattccac ccctaaactg 
     3001 ggggatacgc ctcacgtaca ccgataaact catctttgga aaaggaaccc gtgtgactgt 
     3061 ggaaccaaga agtcagcctc ataccaaacc atccgttttt gtcatgaaaa atggaacaaa 
     3121 tgtcgcttgt ctggtgaagg aattttaccc caaggatata agaataaatc tcgtgtcatc 
     3181 caagaagata acagagtttg atcctgctat tgtcatctct cccggtggga agtacaatgc 
     3241 tgtcaagctt ggtaaatatg aagattcaaa ttcagtgaca tgttcagttc aacacgacaa 
     3301 taaaactgtg cactccactg actttgaagt gaagacagat tctacagatc acgtaaaacc 
     3361 aaaggaaact gaaaacacaa agcaaccttc aaagagctgc cataaaccca aagccatagt 
     3421 tcataccgag aaggtgaaca tgatgtccct cacagtgctt gggctacgaa tgctgtttgc 
     3481 aaagactgtt gccgtcaatt ttctcttgac tgccaagtta tttttcttgt aatctagagg 
     3541 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 
     3601 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 
     3661 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 
     3721 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 
     3781 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 
     3841 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
     3901 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     3961 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag 
     4021 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     4081 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     4141 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     4201 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     4261 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
     4321 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     4381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     4441 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     4501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     4561 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     4621 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 
     4681 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc 
     4741 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac 
     4801 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac 
     4861 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg 
     4921 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac 
     4981 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag 
     5041 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga 
     5101 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     5161 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     5221 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     5281 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     5341 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     5401 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     5461 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     5521 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5581 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     5641 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     5701 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     5761 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     5821 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     5881 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     5941 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 
     6001 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     6061 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     6121 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     6181 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     6241 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     6301 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     6361 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6421 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6481 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6541 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6601 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6661 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     6721 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     6781 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     6841 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     6901 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     6961 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     7021 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     7081 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     7141 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     7201 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     7261 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     7321 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     7381 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     7441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     7501 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//
