LOCUS       pGJM\12\=pPIC9+SFA-8\(N-term)\+BN\(C-term)        8367 bp    DNA     circular      9-SEP-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|328638018|
COMMENT     VNTDBDATE|600782961|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGJM 12 =pPIC9+SFA-8 (N-term) +BN (C-term)|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     promoter        6802..7749
                     /vntifkey="29"
                     /label=5'-AOX1\promoter
     misc_feature    31..364
                     /vntifkey="21"
                     /label=3'\AOX1\transc.\term.
     CDS             complement(758..3292)
                     /vntifkey="4"
                     /label=HIS4\ORF
     CDS             3648..4404
                     /vntifkey="4"
                     /label=3'\AOX1\fragment
     rep_origin      complement(4812..5486)
                     /vntifkey="33"
                     /label=ColE1\origin
     CDS             complement(5631..6491)
                     /vntifkey="4"
                     /label=Ampicillin\resistance\gene
     CDS             8017..8367
                     /gene="SFA8"
                     /codon_start=1
                     /product="pre-pro-seed albumin"
                     /protein_id="CAA40015.1"
                     /db_xref="GI:18821"
                     /db_xref="SWISS-PROT:P23110"
                     /vntifkey="4"
                     /label=CDS(SFA8)_1
     mat_peptide     8017..8367
                     /gene="SFA8"
                     /product="seed albumin"
                     /vntifkey="84"
                     /label=Mat_Peptide(SFA8)_1
     misc_feature    7750..8017
                     /vntifkey="21"
                     /label=a-factor\secretion\signal
BASE COUNT     2246 a      1972 c      1911 g      2238 t 
ORIGIN
        1 aattccctag ggcggccgcg aattaattcg ccttagacat gactgttcct cagttcaagt 
       61 tgggcactta cgagaagacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca 
      121 tttgcctgag agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat 
      181 aggatttttt ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc 
      241 gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc 
      301 ttggtatttc ccactcctct tcagagtaca gaagattaag tgagaagttc gtttgtgcaa 
      361 gcttatcgat aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc 
      421 accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc 
      481 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc 
      541 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct 
      601 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc 
      661 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat 
      721 ctatcgaatc taaatgtaag ttaaaatctc taaataatta aataagtccc agtttctcca 
      781 tacgaacctt aacagcattg cggtgagcat ctagaccttc aacagcagcc agatccatca 
      841 ctgcttggcc aatatgtttc agtccctcag gagttacgtc ttgtgaagtg atgaacttct 
      901 ggaaggttgc agtgttaact ccgctgtatt gacgggcata tccgtacgtt ggcaaagtgt 
      961 ggttggtacc ggaggagtaa tctccacaac tctctggaga gtaggcacca acaaacacag 
     1021 atccagcgtg ttgtacttga tcaacataag aagaagcatt ctcgatttgc aggatcaagt 
     1081 gttcaggagc gtactgattg gacatttcca aagcctgctc gtaggttgca accgataggg 
     1141 ttgtagagtg tgcaatacac ttgcgtacaa tttcaaccct tggcaactgc acagcttggt 
     1201 tgtgaacagc atcttcaatt ctggcaagct ccttgtctgt catatcgaca gccaacagaa 
     1261 tcacctggga atcaatacca tgttcagctt gagacagaag gtctgaggca acgaaatctg 
     1321 gatcagcgta tttatcagca ataactagaa cttcagaagg cccagcaggc atgtcaatac 
     1381 tacacagggc tgatgtgtca ttttgaacca tcatcttggc agcagtaacg aactggtttc 
     1441 ctggaccaaa tattttgtca cacttaggaa cagtttctgt tccgtaagcc atagcagcta 
     1501 ctgcctgggc gcctcctgct agcacgatac acttagcacc aaccttgtgg gcaacgtaga 
     1561 tgacttctgg ggtaagggta ccatccttct taggtggaga tgcaaaaaca atttctttgc 
     1621 aaccagcaac tttggcagga acacccagca tcagggaagt ggaaggcaga attgcggttc 
     1681 caccaggaat atagaggcca actttctcaa taggtcttgc aaaacgagag cagactacac 
     1741 cagggcaagt ctcaacttgc aacgtctccg ttagttgagc ttcatggaat ttcctgacgt 
     1801 tatctataga gagatcaatg gctctcttaa cgttatctgg caattgcata agttcctctg 
     1861 ggaaaggagc ttctaacaca ggtgtcttca aagcgactcc atcaaacttg gcagttagtt 
     1921 ctaaaagggc tttgtcacca ttttgacgaa cattgtcgac aattggtttg actaattcca 
     1981 taatctgttc cgttttctgg ataggacgac gaagggcatc ttcaatttct tgtgaggagg 
     2041 ccttagaaac gtcaattttg cacaattcaa tacgaccttc agaagggact tctttaggtt 
     2101 tggattcttc tttaggttgt tccttggtgt atcctggctt ggcatctcct ttccttctag 
     2161 tgacctttag ggacttcata tccaggtttc tctccacctc gtccaacgtc acaccgtact 
     2221 tggcacatct aactaatgca aaataaaata agtcagcaca ttcccaggct atatcttcct 
     2281 tggatttagc ttctgcaagt tcatcagctt cctccctaat tttagcgttc aacaaaactt 
     2341 cgtcgtcaaa taaccgtttg gtataagaac cttctggagc attgctctta cgatcccaca 
     2401 aggtggcttc catggctcta agaccctttg attggccaaa acaggaagtg cgttccaagt 
     2461 gacagaaacc aacacctgtt tgttcaacca caaatttcaa gcagtctcca tcacaatcca 
     2521 attcgatacc cagcaacttt tgagttgctc cagatgtagc acctttatac cacaaaccgt 
     2581 gacgacgaga ttggtagact ccagtttgtg tccttatagc ctccggaata gactttttgg 
     2641 acgagtacac caggcccaac gagtaattag aagagtcagc caccaaagta gtgaatagac 
     2701 catcggggcg gtcagtagtc aaagacgcca acaaaatttc actgacaggg aactttttga 
     2761 catcttcaga aagttcgtat tcagtagtca attgccgagc atcaataatg gggattatac 
     2821 cagaagcaac agtggaagtc acatctacca actttgcggt ctcagaaaaa gcataaacag 
     2881 ttctactacc gccattagtg aaacttttca aatcgcccag tggagaagaa aaaggcacag 
     2941 cgatactagc attagcgggc aaggatgcaa ctttatcaac cagggtccta tagataaccc 
     3001 tagcgcctgg gatcatcctt tggacaactc tttctgccaa atctaggtcc aaaatcactt 
     3061 cattgatacc attattgtac aacttgagca agttgtcgat cagctcctca aattggtcct 
     3121 ctgtaacgga tgactcaact tgcacattaa cttgaagctc agtcgattga gtgaacttga 
     3181 tcaggttgtg cagctggtca gcagcatagg gaaacacggc ttttcctacc aaactcaagg 
     3241 aattatcaaa ctctgcaaca cttgcgtatg caggtagcaa gggaaatgtc atacttgaag 
     3301 tcggacagtg agtgtagtct tgagaaattc tgaagccgta tttttattat cagtgagtca 
     3361 gtcatcagga gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg 
     3421 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg 
     3481 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt 
     3541 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc 
     3601 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga gtatctatga 
     3661 ttggaagtat gggaatggtg atacccgcat tcttcagtgt cttgaggtct cctatcagat 
     3721 tatgcccaac taaagcaacc ggaggaggag atttcatggt aaatttctct gacttttggt 
     3781 catcagtaga ctcgaactgt gagactatct cggttatgac agcagaaatg tccttcttgg 
     3841 agacagtaaa tgaagtccca ccaataaaga aatccttgtt atcaggaaca aacttcttgt 
     3901 ttcgaacttt ttcggtgcct tgaactataa aatgtagagt ggatatgtcg ggtaggaatg 
     3961 gagcgggcaa atgcttacct tctggacctt caagaggtat gtagggtttg tagatactga 
     4021 tgccaacttc agtgacaacg ttgctatttc gttcaaacca ttccgaatcc agagaaatca 
     4081 aagttgtttg tctactattg atccaagcca gtgcggtctt gaaactgaca atagtgtgct 
     4141 cgtgttttga ggtcatcttt gtatgaataa atctagtctt tgatctaaat aatcttgacg 
     4201 agccaaggcg ataaataccc aaatctaaaa ctcttttaaa acgttaaaag gacaagtatg 
     4261 tctgcctgta ttaaacccca aatcagctcg tagtctgatc ctcatcaact tgaggggcac 
     4321 tatcttgttt tagagaaatt tgcggagatg cgatatcgag aaaaaggtac gctgatttta 
     4381 aacgtgaaat ttatctcaag atctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 
     4441 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 
     4501 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 
     4561 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 
     4621 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 
     4681 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     4741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     4801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     4861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     4921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     4981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     5041 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag 
     5101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     5161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     5221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     5281 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     5341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     5401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     5461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     5521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     5581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     5641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     5701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     5761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     5821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     5881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     5941 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     6001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     6061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     6121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     6181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     6241 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     6301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     6361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     6421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     6481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     6541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     6601 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 
     6661 aaaaataggc gtatcacgag gccctttcgt cttcaagaat taattctcat gtttgacagc 
     6721 ttatcatcga taagctgact catgttggta ttgtgaaata gacgcagatc gggaacactg 
     6781 aaaaataaca gttattattc gagatctaac atccaaagac gaaaggttga atgaaacctt 
     6841 tttgccatcc gacatccaca ggtccattct cacacataag tgccaaacgc aacaggaggg 
     6901 gatacactag cagcagaccg ttgcaaacgc aggacctcca ctcctcttct cctcaacacc 
     6961 cacttttgcc atcgaaaaac cagcccagtt attgggcttg attggagctc gctcattcca 
     7021 attccttcta ttaggctact aacaccatga ctttattagc ctgtctatcc tggcccccct 
     7081 ggcgaggttc atgtttgttt atttccgaat gcaacaagct ccgcattaca cccgaacatc 
     7141 actccagatg agggctttct gagtgtgggg tcaaatagtt tcatgttccc caaatggccc 
     7201 aaaactgaca gtttaaacgc tgtcttggaa cctaatatga caaaagcgtg atctcatcca 
     7261 agatgaacta agtttggttc gttgaaatgc taacggccag ttggtcaaaa agaaacttcc 
     7321 aaaagtcgcc ataccgtttg tcttgtttgg tattgattga cgaatgctca aaaataatct 
     7381 cattaatgct tagcgcagtc tctctatcgc ttctgaaccc cggtgcacct gtgccgaaac 
     7441 gcaaatgggg aaacacccgc tttttggatg attatgcatt gtctccacat tgtatgcttc 
     7501 caagattctg gtgggaatac tgctgatagc ctaacgttca tgatcaaaat ttaactgttc 
     7561 taacccctac ttgacagcaa tatataaaca gaaggaagct gccctgtctt aaaccttttt 
     7621 ttttatcatc attattagct tactttcata attgcgactg gttccaattg acaagctttt 
     7681 gattttaacg acttttaacg acaacttgag aagatcaaaa aacaactaat tattcgaagg 
     7741 atccaaacga tgagatttcc ttcaattttt actgcagttt tattcgcagc atcctccgca 
     7801 ttagctgctc cagtcaacac tacaacagaa gatgaaacgg cacaaattcc ggctgaagct 
     7861 gtcatcggtt actcagattt agaaggggat ttcgatgttg ctgttttgcc attttccaac 
     7921 agcacaaata acgggttatt gtttataaat actactattg ccagcattgc tgctaaagaa 
     7981 gaaggggtat ctctcgagaa aagagaggct gaagctccct atggccgagg tagaactgaa 
     8041 tccggatgct atcagcagat ggaggaggcg gagatgctca accactgcgg gatgtacctc 
     8101 atgaaaaatt taggagaaag gagccaagtt agtcccagaa tgagagaaga ggatcacaag 
     8161 caactttgct gcatgcaact gaagaaccta gatgagaagt gcatgtgcga aggcttaagg 
     8221 atgatgatga ggatgatgca acagaaggag atgcaacccc gaggggagca gatgcgaagg 
     8281 atgatgaggc tggccgagaa tatcccttcc cgctgcaacc tcagtcccat gagatgcccc 
     8341 atgggtggct ccattgccgg gttctga 
//
