LOCUS Exported 9928 bp ds-DNA circular SYN 28-MAY-2019 DEFINITION Ligation of ECFP into pFuGW. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9928) AUTHORS . TITLE Direct Submission JOURNAL Exported May 28, 2019 from SnapGene 4.2.4 http://www.snapgene.com COMMENT Vector molecule: Fragment 2 Fragment ends: blunt Fragment size: 9161 Insert molecule: mKATE Fragment ends: blunt Fragment size: 708 FEATURES Location/Qualifiers source 1..9928 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 239..815 /label=CMVp /note="CMV_immearly_promoter" misc_feature 318..605 /label=CAG_enhancer misc_feature 835..2601 /label=5' Viral freatures misc_feature 835..1015 /label=5'LTR /note="HIV-1_5_LTR" misc_feature 1126..1170 /label=Psi misc_feature 1680..1913 /label=RRE misc_feature 2444..2459 /label=cPPT misc_feature 2629..3829 /label=hUbCp /note="Ubiquitin-C promoter" misc_feature 3854..3862 /label=KOZAK misc_feature 3860..4576 /label=ECFP /note="(48 diff)" misc_feature 4622..5209 /label=WPRE /note="The WPRE seems to increase the expression of the marker gene when introduced in its 3' untranslated region. This element is believed to exert some posttranslational regulation." misc_feature 5396..5417 /label=U3PPT /note="reverse transcriptase-associated RNase H cleavage of the RNA at the polypurine tract (PPT), a short (ca. 11 to 19 nucleotides [nt]) string of purines located at the upstream border of the 3a€² long terminal repeat" misc_feature 5396..5411 /label=cPPT misc_feature 5733..5913 /label=del3'LTR /note="truncHIV-1_3_LTR" misc_feature 5942..6169 /label=bGH-Ter /note="bGH_PA_terminator" misc_feature complement(6658..6873) /label=SV40_enhancer misc_feature 6670..6938 /label=SV40p misc_feature 6837..6914 /label=SV40-Ori misc_feature 7032..7099 /label=EM7p misc_feature 7100..7471 /label=Bleo misc_feature 7607..7726 /label=SV40-pA misc_feature complement(8965..9795) /label=Amp ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt 2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg 2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga 2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc 2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg 2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc 2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga 3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc 3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt 3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt 3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg 3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc 3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg 3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc 3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt 3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc 3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct 3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc 3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg 3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt 3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacaggatcc 3841 ccgggtaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 3901 catcctggtc gagctggacg gcgacgtaaa cggccacagg ttcagcgtgt ccggcgaggg 3961 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 4021 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg 4081 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 4141 ccaggagcgt accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 4201 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 4261 cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg tctatatcac 4321 cgccgacaag cagaagaacg gcatcaaggc ccacttcaag atccgccaca acatcgagga 4381 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 4441 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 4501 gaagcgcgat cacatggtcc tgaaggagtt cgtgaccgcc gccgggatca ctctcggcat 4561 ggacgagctg tacaagtaaa gcggccgcga ctctagaatt cgatatcaag cttatcgata 4621 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 4681 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 4741 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 4801 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 4861 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 4921 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 4981 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 5041 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 5101 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 5161 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc 5221 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 5281 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 5341 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 5401 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 5461 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga 5521 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg 5581 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga 5641 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc 5701 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag 5761 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 5821 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 5881 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc 5941 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 6001 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 6061 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 6121 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 6181 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 6241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 6301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 6361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 6421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 6481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 6541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 6601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 6661 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 6721 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 6781 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 6841 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 6901 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 6961 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 7021 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga 7081 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg 7141 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt 7201 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca 7261 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct 7321 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat 7381 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa 7441 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac 7501 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 7561 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 7621 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 7681 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 7741 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 7801 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 7861 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7921 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7981 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 8041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 8101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 8161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 8221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 8281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 8341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 8401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 8461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 8521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 8581 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 8641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 8701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8821 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8881 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8941 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 9001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 9061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 9121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 9181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 9241 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 9301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 9361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 9421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 9481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 9541 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 9601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 9661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 9721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 9781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 9841 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9901 cacatttccc cgaaaagtgc cacctgac //