LOCUS       Exported                9087 bp ds-DNA     circular SYN 28-MAY-2019
DEFINITION  rFuGW-AscI-KanR filler-SbfI-mKate2 [pLN383 Forward library plasmid; 
            corrected].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2019 from SnapGene 4.2.4
            http://www.snapgene.com
COMMENT     Vector molecule:  VLN490
               Fragment ends: AscI and SbfI
               Fragment size: 8833
            Insert molecule:  KanR filler
               Fragment ends: AscI and SbfI
               Fragment size: 262
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(121..708)
                     /label=WPRE
                     /note="The WPRE seems to increase the expression of the 
                     marker gene when introduced in its 3' untranslated region. 
                     This element is believed to exert some posttranslational 
                     regulation."
     primer_bind     616..635
                     /label=Pi5
     primer_bind     731..750
                     /label=PseqF(490)
     misc_feature    1009..1059
                     /label=lateADEp
                     /note="major late promoter of adenovirus"
     misc_feature    join(1055..9087,1..53)
                     /label=TSS
     misc_feature    1060..1105
                     /label=5' UTR
     primer_bind     complement(1080..1100)
                     /label=Pi7
     misc_signal     1113..1118
                     /label=KOZAK
     gene            1122..1829
                     /label=mKATE
     CDS             1857..1890
                     /gene="HSV1-pA'"
                     /label=HSV1-pA''
     misc_feature    complement(1893..3654)
                     /label=5' Viral freatures'
     misc_feature    complement(2030..2045)
                     /label=cPPT
     misc_feature    complement(2576..2809)
                     /label=RRE
     misc_feature    complement(3319..3363)
                     /label=Psi
     misc_feature    complement(3474..3654)
                     /label=5'LTR
                     /note="HIV-1_5_LTR"
     misc_feature    complement(3674..4250)
                     /label=CMVp
                     /note="CMV_immearly_promoter"
     misc_feature    complement(3884..4171)
                     /label=CAG_enhancer
     misc_feature    4552..4580
                     /label=Ampp
     misc_feature    4622..5482
                     /label=Amp
     misc_feature    complement(6691..6810)
                     /label=SV40-pA
     misc_feature    complement(6946..7317)
                     /label=Bleo
     misc_feature    complement(7318..7385)
                     /label=EM7p
     misc_feature    complement(7479..7747)
                     /label=SV40p
     misc_feature    complement(7503..7580)
                     /label=SV40-Ori
     misc_feature    7544..7759
                     /label=SV40_enhancer
     misc_feature    complement(8248..8475)
                     /label=bGH-Ter
                     /note="bGH_PA_terminator"
     misc_feature    complement(8504..8684)
                     /label=del3'LTR
                     /note="truncHIV-1_3_LTR"
     misc_feature    complement(9000..9021)
                     /label=U3PPT
                     /note="reverse transcriptase-associated RNase H cleavage of
                     the RNA at the polypurine tract (PPT), a short (ca. 11 to 
                     19 nucleotides [nt]) string of purines located at the 
                     upstream border of the 3a€² long terminal repeat"
     misc_feature    complement(9006..9021)
                     /label=cPPT
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg ccctgatgct cttcgtccag atcatcctga
      781 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
      841 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
      901 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
      961 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga ggcgcgccag acgctagcgg
     1021 ggggctataa aagggggtgg gggcgttcgt cctcactcta gatctgcgat ctaagtaagc
     1081 ttggcattcc ggtactgttg gtaaaccagc tcgccaccat ggtgtctaag ggcgaagagc
     1141 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     1201 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     1261 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     1321 acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     1381 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     1441 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     1501 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca
     1561 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc
     1621 tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg
     1681 ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg
     1741 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     1801 tccctagcaa actggggcac aaacttaatt gataaaccgg ttgcagtccc gggtaagcgg
     1861 gactctgggg ttcgaaatga ccgaccaagc attaaccaaa ctggatctct gctgtccctg
     1921 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     1981 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     2041 cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga
     2101 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     2161 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     2221 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     2281 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     2341 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     2401 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     2461 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     2521 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     2581 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     2641 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     2701 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     2761 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     2821 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     2881 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     2941 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     3001 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     3061 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     3121 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     3181 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     3241 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     3301 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     3361 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     3421 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     3481 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     3541 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     3601 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     3661 ggcaaaacgc gctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     3721 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     3781 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     3841 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     3901 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     3961 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     4021 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     4081 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     4141 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     4201 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat
     4261 atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc
     4321 aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga
     4381 cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg
     4441 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
     4501 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4561 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4621 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4681 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4741 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4801 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4861 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4921 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4981 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5041 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5101 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5161 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5221 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5281 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5341 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5401 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5461 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5521 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5581 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5761 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6361 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6421 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6481 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6541 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     6601 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     6661 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     6721 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6781 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
     6841 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     6901 ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc
     6961 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
     7021 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
     7081 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
     7141 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
     7201 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
     7261 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt
     7321 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
     7381 aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag
     7441 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc
     7501 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     7561 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     7621 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     7681 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7741 ccacacccta actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca
     7801 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     7861 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7921 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     7981 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8041 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8101 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8161 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
     8221 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     8281 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     8341 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     8401 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     8461 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctgctaga gattttccac
     8521 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     8581 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     8641 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca gtccggatgc
     8701 agctctcggg ccatgtgatg aaatgctagg cggctgtcaa acctccactc taatacttct
     8761 ctctccgggt catccatccc atgcaggctc acagggtgta acaagcgggt gttctctcct
     8821 tcattggctt cttctacctt ctcttgctca actggtacta gcttgtagca ccatccaaag
     8881 gtcagtggat atctgatccc tggccctggt gtgtagttct gccaatcagg gaagtagcct
     8941 tgtgtgtggt agatccacag atcaaggata tcttgtcttc gttgggagtg aattagccct
     9001 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt
     9061 ggtcttaaag gtacctgagg tgtgact
//
