LOCUS       Exported               11231 bp ds-DNA     circular SYN 10-MAR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Stasevich
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UBC_human promoter
                     /label=UBC_human
     primer_bind     2536..2556
                     /label=TM_001
                     /note="TM_001"
     primer_bind     2536..2556
                     /label=TM_001
     primer_bind     2618..2692
                     /label=KN_040
     primer_bind     complement(2622..2688)
                     /label=KN_041
     CDS             2628..2630
                     /codon_start=1
                     /label=+0
                     /translation="M"
     primer_bind     complement(2633..2640)
                     /label=KN_028
     misc_feature    2696..2736
                     /label=FSS
     CDS             2696..2701
                     /codon_start=1
                     /label=-1 Frame
                     /translation="FF"
     misc_feature    2703..2710
                     /label=Spacer
     misc_feature    2711..2735
                     /label=RNA Modulator
     misc_feature    2736..2779
                     /label=gag +0
     CDS             2787..2810
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2813..2869
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2871..2894
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2897..2953
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2955..2978
                     /codon_start=1
                     /label=SpaghettiMonster_actb
                     /translation="DYKDDDDK"
     misc_feature    2955..2978
                     /label=insert
     CDS             2955..2978
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     2980..3002
                     /label=KN_056
     CDS             2981..3037
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3039..3062
                     /codon_start=1
                     /label=SpaghettiMonster_actb
                     /translation="DYKDDDDK"
     misc_feature    3039..3062
                     /label=insert
     CDS             3039..3062
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3064..3083)
                     /label=KN_055
     CDS             3065..3121
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3123..3146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3149..3205
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3207..3230
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3233..3289
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3291..3314
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3317..3373
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3375..3398
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     3398..3422
                     /label=KN_056
     CDS             3401..3457
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3459..3482
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3485..3541
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3543..3566
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3569..3625
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3627..3650
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3653..3709
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3711..3734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     3730..3752
                     /label=KN_039
     CDS             3737..3793
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     3804..3824
                     /label=KN_027
     primer_bind     3806..3831
                     /label=KN_033
     gene            3808..5877
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /label=GNAS
                     /note="GNAS complex locus; Derived by automated 
                     computational analysis using gene prediction method: 
                     BestRefSeq."
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
     mRNA            3808..5876
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="GNAS complex locus, transcript variant 2"
                     /label=GNAS mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:117938767"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
     mRNA            3808..5876
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="GNAS complex locus, transcript variant 2"
                     /label=GNAS mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:117938758"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
     CDS             3809..5876
                     /codon_start=1
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="protein GNAS isoform XLas"
                     /label=XXL
                     /note="isoform XLas is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="GI:117938759"
                     /db_xref="CCDS:CCDS46622.1"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
                     /protein_id="NP_536350.2"
                     /translation="MGVRNCLYGNNMSGQRDIPPEIGEQPEQPPLEAPGAAAPGAGPSP
                     AEEMETEPPHNEPIPVENDGEACGPPEVSRPNFQVLNPAFREAGAHGSYSPPPEEAMPF
                     EAEQPSLGGFWPTLEQPGFPSGVHAGLEAFGPALMEPGAFSGARPGLGGYSPPPEEAMP
                     FEFDQPAQRGCSQLLLQVPDLAPGGPGAAGVPGAPPEEPQALRPAKAGSRGGYSPPPEE
                     TMPFELDGEGFGDDSPPPGLSRVIAQVDGSSQFAAVAASSAVRLTPAANAPPLWVPGAI
                     GSPSQEAVRPPSNFTGSSPWMEISGPPFEIGSAPAGVDDTPVNMDSPPIALDGPPIKVS
                     GAPDKRERAERPPVEEEAAEMEGAADAAEGGKVPSPGYGSPAAGAASADTAARAAPAAP
                     ADPDSGATPEDPDSGTAPADPDSGAFAADPDSGAAPAAPADPDSGAAPDAPADPDSGAA
                     PDAPADPDAGAAPEAPAAPAAAETRAAHVAPAAPDAGAPTAPAASATRAAQVRRAASAA
                     PASGARRKIHLRPPSPEIQAADPPTPRPTRASAWRGKSESSRGRRVYYDEGVASSDDDS
                     SGDESDDGTSGCLRWFQHRRNRRRRKPQRNLLRNFLVQAFGGCFGRSESPQPKASRSLK
                     VKKVPLAEKRRQMRKEALEKRAQKRAEKKRSKLIDKQLQDEKMGYMCTHRLLLL"
     primer_bind     complement(3822..3844)
                     /label=KN_038
     CDS             3996..5876
                     /codon_start=1
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="protein ALEX isoform Alex"
                     /label=ALEXX
                     /note="isoform Alex is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="GI:117938768"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
                     /protein_id="NP_001070958.1"
                     /translation="MMARPVDPQRSPDPTFRSSTRHSGKLEPMEATAHLLRKQCPSRLN
                     SPAWEASGLHWSSLDSPVGSMQALRPSAQHSWSPEPSVVPDQAWEDTALHQKKLCPLSL
                     TSLPREAAVNFSYRSQTLLQEAQVLQGSPELLPRSPKPSGLQRLAPEEATALPLRRLCH
                     LSLMEKDLGTTAHPRGFPELSHKSTAAASSRQSRPRVRSASLPPRTRLPSGSQAPSAAH
                     PKRLSDLLLTSRAAAPGWRSPDPRSRLAAPPLGSTTLPSTWTAPQSRLTARPSRSPEPQ
                     IRESEQRDPQLRRKQQRWKEPLMPRREEKYPLRGTDPLPPGQPQRIPLPGQPLQPQPIL
                     TPGQPQKIPTPGQHQPILTPGHSQPIPTPGQPLPPQPIPTPGRPLTPQPIPTPGRPLTP
                     QPIQMPGRPLRLPPPLRLLRPGQPMSPQLRQTQGLPLPQPLLPPGQPKSAGRPLQPLPP
                     GPDARSISDPPAPRSRLPIRLLRGLLARLPGGASPRAAAAAACTTMKGWPAATMTPAET
                     SPTMGPPDASAGFSIGEIAAAESPSATYSATFSCKPSGAASVDLRVPSPKPRALSRSRR
                     YPWRRSADRCAKKPWRSGPRSAQRRNAVSSSTNNSRTKRWATCVRTACCF"
     primer_bind     4722..4739
                     /label=KN_063
     primer_bind     complement(4939..4956)
                     /label=KN_034
     primer_bind     5620..5627
                     /label=KN_027
     primer_bind     complement(5858..5884)
                     /label=KN_028
     protein_bind    5887..5916
                     /label=Zipcode
     primer_bind     complement(5960..5980)
                     /label=TM_002
     protein_bind    6343..6365
                     /label=MS2_1
     protein_bind    6396..6418
                     /label=MS2_2
     protein_bind    6449..6471
                     /label=MS2_3
     protein_bind    6503..6523
                     /label=MS2_4
     protein_bind    6555..6577
                     /label=MS2_5
     protein_bind    6608..6630
                     /label=MS2_6
     protein_bind    6661..6683
                     /label=MS2_7
     protein_bind    6714..6736
                     /label=MS2_8
     protein_bind    6767..6789
                     /label=MS2_9
     protein_bind    6820..6842
                     /label=MS2_10
     protein_bind    6873..6895
                     /label=MS2_11
     protein_bind    6926..6948
                     /label=MS2_12
     protein_bind    6979..7001
                     /label=MS2_13
     primer_bind     6981..6989
                     /label=KN_010
     protein_bind    7032..7054
                     /label=MS2_14
     protein_bind    7085..7107
                     /label=MS2_15
     protein_bind    7138..7160
                     /label=MS2_16
     protein_bind    7191..7213
                     /label=MS2_17
     protein_bind    7244..7266
                     /label=MS2_18
     protein_bind    7297..7319
                     /label=MS2_19
     protein_bind    7350..7372
                     /label=MS2_20
     protein_bind    7403..7425
                     /label=MS2_21
     protein_bind    7456..7478
                     /label=MS2_22
     protein_bind    7509..7531
                     /label=MS2_23
     protein_bind    7562..7584
                     /label=MS2_24
     misc_feature    7621..8209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8092..8103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8284..8517
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        8722..8826
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8827..9687
                     /label=AmpR
     CDS             9025..9684
                     /label=AmpR(1)
     rep_origin      9782..10464
                     /label=ColE1 origin
     rep_origin      9832..10505
                     /label=pUC_replication_origin
     rep_origin      9858..10446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10052..10059)
                     /label=KN_028
     protein_bind    10857..10878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10893..10923
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_binding    10927..10949
                     /label=LacO
     protein_bind    10931..10947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10955..10971
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg agcaccgcct
     2641 cctctggcac agccagtagt gggactgcat cctcaggtgc gggtaccctg catggttttt
     2701 tagggaagat ctggccttcc cacaagggaa ggccagggaa ttttcttcag agcagaccag
     2761 agccaacagc cgcaccgttt ctagaggact acaaggacga cgacgacaaa gggaagaact
     2821 tttgtcaaag aattatcatc tcgagaacga agtggctcgt ctcaagaaag gattacaaag
     2881 acgacgacga caaggggaag aactgctttc aaagaattac cacctggaaa acgaggtcgc
     2941 gagactcaaa aagagattac aaagacgacg acgacaaggg gaagaattac tcagcaaaaa
     3001 ttatcatctg gaaaacgagg ttgcgagact caaaaaggga ctacaaagac gacgacgaca
     3061 aagcgaagaa cttttgtcca agaattatca tctggagaac gaagtggctc gtctcaagaa
     3121 aggattacaa ggacgacgac gacaagggga agaactgctt tcaaagaatt accacctgga
     3181 aaatgaggtg gctagactca aaaagggatt acaaggacga cgacgacaag gggaggaact
     3241 actttcaaag aattaccacc tcgaaaacga agtggctcga cttaagaaag gactacaaag
     3301 acgacgacga caaggggagg aattgctatc gaaaaattat catcttgaga acgaagttgc
     3361 taggctcaaa aagcgattac aaggacgacg acgacaagcg gaagaattac tgtccaaaaa
     3421 ttatcatctg gaaaatgagg tggcgagact caaaaaggga ctacaaggac gacgacgaca
     3481 aaccgaggaa ctgctctcga agaactatca tcttgaaaat gaggtcgctc gactcaaaaa
     3541 gagattacaa ggacgacgac gacaagcaga agagctatta tctaaaaact accacctcga
     3601 aaatgaggtg gcacgcctca aaaagggatt acaaagacga cgacgacaaa aggaagagct
     3661 actatccaag aattatcatc ttgagaacga ggtggcgcgt ctcaagaagc gactacaagg
     3721 acgacgacga caaagagagg agttgcttag caaaaattat catttggaga acgaagtcgc
     3781 acgactcaag aaaactagtg gcggtcatat gggcgtgcgc aactgcctct acggcaataa
     3841 tatgtcagga caacgcgata tcccccctga aatcggggaa cagcccgagc aaccaccttt
     3901 ggaggcccca ggggcagctg cccccggtgc tgggcctagc ccagccgaag agatggagac
     3961 cgaaccgcct cacaacgagc ccatccccgt cgagaatgat ggcgaggcct gtggaccccc
     4021 agaggtctcc agacccaact ttcaggtcct caacccggca ttcagggaag ctggagccca
     4081 tggaagctac agcccacctc ctgaggaagc aatgcccttc gaggctgaac agcccagctt
     4141 gggaggcttc tggcctacac tggagcagcc tggattcccc agtggggtcc atgcaggcct
     4201 tgaggccttc ggcccagcac tcatggagcc cggagccttc agtggtgcca gaccaggcct
     4261 gggaggatac agccctccac cagaagaagc tatgcccttt gagtttgacc agcctgccca
     4321 gagaggctgc agtcaacttc tcttacaggt cccagacctt gctccaggag gcccaggtgc
     4381 tgcaggggtc cccggagctc ctcccgagga gccccaagcc ctcaggcctg caaaggctgg
     4441 ctccagagga ggctacagcc ctccccctga ggagactatg ccatttgagc ttgatggaga
     4501 aggatttggg gacgacagcc cacccccggg gctttcccga gttatcgcac aagtcgacgg
     4561 cagcagccag ttcgcggcag tcgcggcctc gagtgcggtc cgcctcactc ccgccgcgaa
     4621 cgcgcctccc ctctgggtcc caggcgccat cggcagccca tcccaagagg ctgtcagacc
     4681 tccttctaac ttcacgggca gcagcccctg gatggagatc tccggacccc cgttcgagat
     4741 tggcagcgcc cccgctgggg tcgacgacac tcccgtcaac atggacagcc ccccaatcgc
     4801 gcttgacggc ccgcccatca aggtctccgg agccccagat aagagagagc gagcagagag
     4861 acccccagtt gaggaggaag cagcagagat ggaaggagcc gctgatgccg cggagggagg
     4921 aaaagtaccc tctccggggt acggatcccc tgccgccggg gcagcctcag cggataccgc
     4981 tgccagggca gcccctgcag ccccagccga tcctgactcc ggggcaaccc cagaagatcc
     5041 cgactccggg acagcaccag ccgatcctga ctccggggca ttcgcagccg atcccgactc
     5101 cggggcagcc cctgccgccc cagccgatcc cgactccggg gcggcccctg acgccccagc
     5161 cgatcccgac tccggggcgg cccctgacgc cccagccgat ccagatgccg gggcggcccc
     5221 tgaggctccc gccgcccctg cggctgctga gacccgggca gcccatgtcg ccccagctgc
     5281 gccagacgca ggggctccca ctgccccagc cgcttctgcc acccgggcag cccaagtccg
     5341 ccgggcggcc tctgcagccc ctgcctccgg ggccagacgc aagatccatc tcagaccccc
     5401 cagccccgag atccaggctg ccgatccgcc tactccgcgg cctactcgcg cgtctgcctg
     5461 gcggggcaag tccgagagca gccgcggccg ccgcgtgtac tacgatgaag gggtggccag
     5521 cagcgacgat gactccagcg gagacgagtc cgacgatggg acctccggat gcctccgctg
     5581 gtttcagcat cggcgaaatc gccgccgccg aaagccccag cgcaacttac tccgcaactt
     5641 tctcgtgcaa gccttcgggg gctgcttcgg tcgatctgag agtccccagc ccaaagcctc
     5701 gcgctctctc aaggtcaaga aggtacccct ggcggagaag cgcagacaga tgcgcaaaga
     5761 agccctggag aagcgggccc agaagcgcgc agagaagaaa cgcagtaagc tcatcgacaa
     5821 acaactccag gacgaaaaga tgggctacat gtgtacgcac cgcctgctgc ttctaggcta
     5881 gctaggcgga ctgttactga gctgcgtttt acaccctttc tttgacaaaa cctaacttgc
     5941 gcagaaaaaa aaaaaataag agacaacatt ggcatggctt tgttttttta aatttttttt
     6001 aaagtttttt tttttttttt tttttttttt tttaagtttt tttgttttgt tttggcgctt
     6061 ttgactcagg atttaaaaac tggaacggtg aaggcgacag cagttggttg gagcaaacat
     6121 cccccaaagt tctacaaatg tggctgagga ctttgtacat tgttttgttt tttttttttt
     6181 ttggttttgt ctttttttaa tagtcattcc aagtatccat gaaataagtg gttacaggaa
     6241 gtccctcacc ctcccaaaag ccacccccac tcctaagagg aggatggtcg cgtccatgcc
     6301 ctgagtccac cccggggaag gtgacaaccg gtaacctaca aacgggtgga ggatcacccc
     6361 acccgacact tcacaatcaa ggggtacaat acacaagggt ggaggaacac cccaccctcc
     6421 agacacatta cacagaaatc caatcaaaca gaagcaccat cagggcttct gctaccaaat
     6481 ttatctcaaa aaactacaac aaggaatcac catcagggat tccctgtgca atatacgtca
     6541 aacgagggcc acgacgggag gacgatcacg cctcccgaat atcggcatgt ctggctttcg
     6601 aattcagtgc gtggagcatc agcccacgca gccaatcaga gtcgaataca agtcgacttt
     6661 cgcgaagagc atcagccttc gcgccattct tacacaaacc acactctccc ctacaggaac
     6721 agcatcagcg ttcctgccca gtacccaact caagaaaatt tatgtcccca tgcagcatca
     6781 gcgcatgggc cccaagaata catccccaac aaaatcacat ccgagcacca acagggctcg
     6841 gagtgttgtt tcttgtccaa ctggacaaac cctccatgga ccatcaggcc atggactctc
     6901 accaacaaga caaaaactac tcttctcgaa gcagcatcag cgcttcgaaa cactcgagca
     6961 tacattgtgc ctatttcttg ggtggacgat cacgccaccc atgctctcac gaatttcaaa
     7021 acacggacaa ggacgagcac caccagggct cgtcgttcca cgtccaatac gattacttac
     7081 ctttcgggat cacgatcacg gatcccgcag ctacatcact tccactcagg acattcaagc
     7141 atgcacgatc acggcatgct ccacaagtct caaccacaga aactaccaaa tgggttcagc
     7201 accagcgaac ccactcctac ctcaaacctc ttcccacaaa actggcaagc aggatcaccg
     7261 cttgcccatt ccaacatacc aaatcaaaaa caattactgg tacagcatca gcgtaccagc
     7321 ccacatctct cactactatc aaaaaccaaa ccgttcagca acagcgaacg gtacacacgg
     7381 aaaaatcaac tggtttacaa atacgaaaga cgatcacgct ttcgtccagc gcaaactatt
     7441 acgaaaaaca tccgacggga agagcaacag ccttcccgcg gcggaaaacc tcacaaaaac
     7501 acgacaaacg gatgcacgaa cacggcatcc gccgacaacc cacaaactta caaccaggca
     7561 aacggtgcag gatcaccgca ccgtacatca aacacctcag atctcatatc gatagatcct
     7621 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7681 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7741 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7801 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7861 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7921 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7981 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8041 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8101 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8161 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
     8221 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8281 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     8341 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8401 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8461 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     8521 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8581 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
     8641 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     8701 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8761 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8821 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8881 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8941 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9001 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9061 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9121 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9181 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9241 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9301 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9361 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9421 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9481 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9541 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9601 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9661 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9721 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9781 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9841 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9901 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9961 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10021 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10081 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10141 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10201 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10261 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10321 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10381 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10441 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10501 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10561 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10621 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10681 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    10741 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10801 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    10861 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    10921 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
    10981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    11041 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    11101 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    11161 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    11221 aacttagtag t
//
