LOCUS       Exported               12266 bp ds-DNA     circular SYN 14-DEC-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Stasevich
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UBC_human promoter
                     /label=UBC_human
     primer_bind     2536..2556
                     /label=TM_001
                     /note="TM_001"
     primer_bind     2536..2556
                     /label=TM_001
     CDS             2628..3674
                     /codon_start=1
                     /label=smFP_HA
                     /translation="MYPYDVPDYAGYPYDVPDYAGYPYDVPDYAGGVSKGEELFTGVVP
                     ILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGGGVQCFS
                     RYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFK
                     EDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEGGYPYDVPDYAGGYPYDV
                     PDYAGGGGYPYDVPDYAGGYPYDVPDYAGGDGSVQLADHYQQNTPIGDGPVLLPDNHYL
                     STQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGYPYDVPDYAGYPYDVPDYAG
                     YPYDVPDYA"
     CDS             2631..2657
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     2631..2657
                     /label=KN_071
     CDS             2661..2687
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2691..2717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3246..3272
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3279..3305
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3318..3344
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3351..3377
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3588..3614
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3618..3644
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3644..3711)
                     /label=TM_014
     CDS             3648..3674
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3653..3675)
                     /label=KN_072
     primer_bind     3717..3727
                     /label=KN_040
     primer_bind     3729..3755
                     /label=KN_070
     misc_feature    3731..3771
                     /label=FSS
     CDS             3731..3736
                     /codon_start=1
                     /label=-1 Frame
                     /translation="FF"
     misc_feature    3738..3745
                     /label=Spacer
     misc_feature    3746..3770
                     /label=RNA Modulator
     primer_bind     complement(3762..3771)
                     /label=KN_070
     misc_feature    3771..3814
                     /label=gag +0
     primer_bind     complement(3795..3815)
                     /label=KN_071
     CDS             3822..3845
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3848..3904
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3906..3929
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3932..3988
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3990..4013
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3990..4013
                     /label=insert
     CDS             3990..4013
                     /codon_start=1
                     /label=SpaghettiMonster_actb
                     /translation="DYKDDDDK"
     CDS             4016..4072
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4074..4097
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4074..4097
                     /label=insert
     CDS             4074..4097
                     /codon_start=1
                     /label=SpaghettiMonster_actb
                     /translation="DYKDDDDK"
     primer_bind     complement(4099..4118)
                     /label=KN_055
     CDS             4100..4156
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4158..4181
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4184..4240
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4242..4265
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4268..4324
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4326..4349
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4352..4408
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4410..4433
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     4433..4457
                     /label=KN_056
     CDS             4436..4492
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4494..4517
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4520..4576
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4578..4601
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4604..4660
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4662..4685
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4688..4744
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4746..4769
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     4765..4787
                     /label=KN_039
     CDS             4772..4828
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     4839..4859
                     /label=KN_027
     primer_bind     4841..4866
                     /label=KN_033
     gene            4843..6912
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /label=GNAS
                     /note="GNAS complex locus; Derived by automated 
                     computational analysis using gene prediction method: 
                     BestRefSeq."
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
     mRNA            4843..6911
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="GNAS complex locus, transcript variant 2"
                     /label=GNAS mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:117938758"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
     mRNA            4843..6911
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="GNAS complex locus, transcript variant 2"
                     /label=GNAS mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:117938767"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
     CDS             4844..6911
                     /codon_start=1
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="protein GNAS isoform XLas"
                     /label=XXL
                     /note="isoform XLas is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="GI:117938759"
                     /db_xref="CCDS:CCDS46622.1"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
                     /protein_id="NP_536350.2"
                     /translation="MGVRNCLYGNNMSGQRDIPPEIGEQPEQPPLEAPGAAAPGAGPSP
                     AEEMETEPPHNEPIPVENDGEACGPPEVSRPNFQVLNPAFREAGAHGSYSPPPEEAMPF
                     EAEQPSLGGFWPTLEQPGFPSGVHAGLEAFGPALMEPGAFSGARPGLGGYSPPPEEAMP
                     FEFDQPAQRGCSQLLLQVPDLAPGGPGAAGVPGAPPEEPQALRPAKAGSRGGYSPPPEE
                     TMPFELDGEGFGDDSPPPGLSRVIAQVDGSSQFAAVAASSAVRLTPAANAPPLWVPGAI
                     GSPSQEAVRPPSNFTGSSPWMEISGPPFEIGSAPAGVDDTPVNMDSPPIALDGPPIKVS
                     GAPDKRERAERPPVEEEAAEMEGAADAAEGGKVPSPGYGSPAAGAASADTAARAAPAAP
                     ADPDSGATPEDPDSGTAPADPDSGAFAADPDSGAAPAAPADPDSGAAPDAPADPDSGAA
                     PDAPADPDAGAAPEAPAAPAAAETRAAHVAPAAPDAGAPTAPAASATRAAQVRRAASAA
                     PASGARRKIHLRPPSPEIQAADPPTPRPTRASAWRGKSESSRGRRVYYDEGVASSDDDS
                     SGDESDDGTSGCLRWFQHRRNRRRRKPQRNLLRNFLVQAFGGCFGRSESPQPKASRSLK
                     VKKVPLAEKRRQMRKEALEKRAQKRAEKKRSKLIDKQLQDEKMGYMCTHRLLLL"
     primer_bind     complement(4857..4879)
                     /label=KN_038
     CDS             5031..6911
                     /codon_start=1
                     /gene="GNAS"
                     /gene_synonym="AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; 
                     PHP1A; PHP1B; PHP1C; POH"
                     /product="protein ALEX isoform Alex"
                     /label=ALEXX
                     /note="isoform Alex is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="GI:117938768"
                     /db_xref="GeneID:2778"
                     /db_xref="HGNC:HGNC:4392"
                     /db_xref="HPRD:00761"
                     /db_xref="MIM:139320"
                     /protein_id="NP_001070958.1"
                     /translation="MMARPVDPQRSPDPTFRSSTRHSGKLEPMEATAHLLRKQCPSRLN
                     SPAWEASGLHWSSLDSPVGSMQALRPSAQHSWSPEPSVVPDQAWEDTALHQKKLCPLSL
                     TSLPREAAVNFSYRSQTLLQEAQVLQGSPELLPRSPKPSGLQRLAPEEATALPLRRLCH
                     LSLMEKDLGTTAHPRGFPELSHKSTAAASSRQSRPRVRSASLPPRTRLPSGSQAPSAAH
                     PKRLSDLLLTSRAAAPGWRSPDPRSRLAAPPLGSTTLPSTWTAPQSRLTARPSRSPEPQ
                     IRESEQRDPQLRRKQQRWKEPLMPRREEKYPLRGTDPLPPGQPQRIPLPGQPLQPQPIL
                     TPGQPQKIPTPGQHQPILTPGHSQPIPTPGQPLPPQPIPTPGRPLTPQPIPTPGRPLTP
                     QPIQMPGRPLRLPPPLRLLRPGQPMSPQLRQTQGLPLPQPLLPPGQPKSAGRPLQPLPP
                     GPDARSISDPPAPRSRLPIRLLRGLLARLPGGASPRAAAAAACTTMKGWPAATMTPAET
                     SPTMGPPDASAGFSIGEIAAAESPSATYSATFSCKPSGAASVDLRVPSPKPRALSRSRR
                     YPWRRSADRCAKKPWRSGPRSAQRRNAVSSSTNNSRTKRWATCVRTACCF"
     primer_bind     5757..5774
                     /label=KN_063
     primer_bind     complement(5974..5991)
                     /label=KN_034
     primer_bind     complement(6893..6919)
                     /label=KN_028
     protein_bind    6922..6951
                     /label=Zipcode
     primer_bind     complement(6995..7015)
                     /label=TM_002
     protein_bind    7378..7400
                     /label=MS2_1
     protein_bind    7431..7453
                     /label=MS2_2
     protein_bind    7484..7506
                     /label=MS2_3
     protein_bind    7538..7558
                     /label=MS2_4
     protein_bind    7590..7612
                     /label=MS2_5
     protein_bind    7643..7665
                     /label=MS2_6
     protein_bind    7696..7718
                     /label=MS2_7
     protein_bind    7749..7771
                     /label=MS2_8
     protein_bind    7802..7824
                     /label=MS2_9
     protein_bind    7855..7877
                     /label=MS2_10
     protein_bind    7908..7930
                     /label=MS2_11
     protein_bind    7961..7983
                     /label=MS2_12
     protein_bind    8014..8036
                     /label=MS2_13
     protein_bind    8067..8089
                     /label=MS2_14
     protein_bind    8120..8142
                     /label=MS2_15
     protein_bind    8173..8195
                     /label=MS2_16
     protein_bind    8226..8248
                     /label=MS2_17
     protein_bind    8279..8301
                     /label=MS2_18
     protein_bind    8332..8354
                     /label=MS2_19
     protein_bind    8385..8407
                     /label=MS2_20
     protein_bind    8438..8460
                     /label=MS2_21
     primer_bind     complement(8457..8470)
                     /label=KN_010
     protein_bind    8491..8513
                     /label=MS2_22
     protein_bind    8544..8566
                     /label=MS2_23
     protein_bind    8597..8619
                     /label=MS2_24
     misc_feature    8656..9244
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9127..9138)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9319..9552
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9757..9861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9862..10722
                     /label=AmpR
     CDS             10060..10719
                     /label=AmpR(1)
     rep_origin      10817..11499
                     /label=ColE1 origin
     rep_origin      10867..11540
                     /label=pUC_replication_origin
     rep_origin      10893..11481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11892..11913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11928..11958
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_binding    11962..11984
                     /label=LacO
     protein_bind    11966..11982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11990..12006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg tacccttatg
     2641 atgtgcccga ttatgctggc tacccttatg atgttcctga ttacgccgga tatccgtatg
     2701 acgtgccaga ctacgcggga ggtgtgagca agggagagga gttgtttacc ggcgtggtcc
     2761 ccatactggt ggagttggat ggcgacgtta atggacataa attctcggtc cgcggcgagg
     2821 gagagggaga cgccaccaat ggcaagctga cgcttaagtt catttgcacg actggaaaat
     2881 tgcctgtccc ctggccgacc ctggtcacca cactgggcgg aggagtgcag tgcttctccc
     2941 gttacccaga ccacatgaag cagcacgatt tcttcaagag cgcaatgccg gagggatacg
     3001 tgcaagaacg gactatttcc ttcaaggatg acggaaccta taagacccgt gcggaggtca
     3061 aattcgaggg tgacaccctg gtgaaccgaa ttgaactcaa aggaatcgat ttcaaggagg
     3121 atggaaatat tctgggtcac aagctggaat acaacttcaa cagccataat gtgtacatta
     3181 cggctgataa gcagaagaac ggcattaagg ccaatttcaa gatccgccac aacgttgagg
     3241 gtggatatcc ctacgacgtg cccgattatg cgggcggata tccgtatgat gttccagatt
     3301 atgctggtgg tggaggctat ccctatgatg tccccgacta cgccggaggt tacccatacg
     3361 acgtgcccga ctacgctgga ggcgatggca gcgtgcagct ggcagatcat tatcaacaga
     3421 atacccccat aggtgatggc cccgttctgc ttccagataa tcactacctt tccacccaga
     3481 gcgtgctttc gaaagacccg aacgaaaagc gtgatcacat ggtcctgctg gagtttgtga
     3541 ccgcggctgg aatcaccctg ggtatggacg aactctacaa gggtggctac ccctacgatg
     3601 tgccggatta cgctggatat ccgtatgacg taccggacta tgccggttac ccgtacgatg
     3661 tcccggacta cgctggaggc ggtggatcag gtggaggagg ttcactgcag gctcgtggta
     3721 ccctgcatgg ttttttaggg aagatctggc cttcccacaa gggaaggcca gggaattttc
     3781 ttcagagcag accagagcca acagccgcac cgtttctaga ggactacaag gacgacgacg
     3841 acaaagggaa gaacttttgt caaagaatta tcatctcgag aacgaagtgg ctcgtctcaa
     3901 gaaaggatta caaagacgac gacgacaagg ggaagaactg ctttcaaaga attaccacct
     3961 ggaaaacgag gtcgcgagac tcaaaaagag attacaaaga cgacgacgac aaggggaaga
     4021 attactcagc aaaaattatc atctggaaaa cgaggttgcg agactcaaaa agggactaca
     4081 aagacgacga cgacaaagcg aagaactttt gtccaagaat tatcatctgg agaacgaagt
     4141 ggctcgtctc aagaaaggat tacaaggacg acgacgacaa ggggaagaac tgctttcaaa
     4201 gaattaccac ctggaaaatg aggtggctag actcaaaaag ggattacaag gacgacgacg
     4261 acaaggggag gaactacttt caaagaatta ccacctcgaa aacgaagtgg ctcgacttaa
     4321 gaaaggacta caaagacgac gacgacaagg ggaggaattg ctatcgaaaa attatcatct
     4381 tgagaacgaa gttgctaggc tcaaaaagcg attacaagga cgacgacgac aagcggaaga
     4441 attactgtcc aaaaattatc atctggaaaa tgaggtggcg agactcaaaa agggactaca
     4501 aggacgacga cgacaaaccg aggaactgct ctcgaagaac tatcatcttg aaaatgaggt
     4561 cgctcgactc aaaaagagat tacaaggacg acgacgacaa gcagaagagc tattatctaa
     4621 aaactaccac ctcgaaaatg aggtggcacg cctcaaaaag ggattacaaa gacgacgacg
     4681 acaaaaggaa gagctactat ccaagaatta tcatcttgag aacgaggtgg cgcgtctcaa
     4741 gaagcgacta caaggacgac gacgacaaag agaggagttg cttagcaaaa attatcattt
     4801 ggagaacgaa gtcgcacgac tcaagaaaac tagtggcggt catatgggcg tgcgcaactg
     4861 cctctacggc aataatatgt caggacaacg cgatatcccc cctgaaatcg gggaacagcc
     4921 cgagcaacca cctttggagg ccccaggggc agctgccccc ggtgctgggc ctagcccagc
     4981 cgaagagatg gagaccgaac cgcctcacaa cgagcccatc cccgtcgaga atgatggcga
     5041 ggcctgtgga cccccagagg tctccagacc caactttcag gtcctcaacc cggcattcag
     5101 ggaagctgga gcccatggaa gctacagccc acctcctgag gaagcaatgc ccttcgaggc
     5161 tgaacagccc agcttgggag gcttctggcc tacactggag cagcctggat tccccagtgg
     5221 ggtccatgca ggccttgagg ccttcggccc agcactcatg gagcccggag ccttcagtgg
     5281 tgccagacca ggcctgggag gatacagccc tccaccagaa gaagctatgc cctttgagtt
     5341 tgaccagcct gcccagagag gctgcagtca acttctctta caggtcccag accttgctcc
     5401 aggaggccca ggtgctgcag gggtccccgg agctcctccc gaggagcccc aagccctcag
     5461 gcctgcaaag gctggctcca gaggaggcta cagccctccc cctgaggaga ctatgccatt
     5521 tgagcttgat ggagaaggat ttggggacga cagcccaccc ccggggcttt cccgagttat
     5581 cgcacaagtc gacggcagca gccagttcgc ggcagtcgcg gcctcgagtg cggtccgcct
     5641 cactcccgcc gcgaacgcgc ctcccctctg ggtcccaggc gccatcggca gcccatccca
     5701 agaggctgtc agacctcctt ctaacttcac gggcagcagc ccctggatgg agatctccgg
     5761 acccccgttc gagattggca gcgcccccgc tggggtcgac gacactcccg tcaacatgga
     5821 cagcccccca atcgcgcttg acggcccgcc catcaaggtc tccggagccc cagataagag
     5881 agagcgagca gagagacccc cagttgagga ggaagcagca gagatggaag gagccgctga
     5941 tgccgcggag ggaggaaaag taccctctcc ggggtacgga tcccctgccg ccggggcagc
     6001 ctcagcggat accgctgcca gggcagcccc tgcagcccca gccgatcctg actccggggc
     6061 aaccccagaa gatcccgact ccgggacagc accagccgat cctgactccg gggcattcgc
     6121 agccgatccc gactccgggg cagcccctgc cgccccagcc gatcccgact ccggggcggc
     6181 ccctgacgcc ccagccgatc ccgactccgg ggcggcccct gacgccccag ccgatccaga
     6241 tgccggggcg gcccctgagg ctcccgccgc ccctgcggct gctgagaccc gggcagccca
     6301 tgtcgcccca gctgcgccag acgcaggggc tcccactgcc ccagccgctt ctgccacccg
     6361 ggcagcccaa gtccgccggg cggcctctgc agcccctgcc tccggggcca gacgcaagat
     6421 ccatctcaga ccccccagcc ccgagatcca ggctgccgat ccgcctactc cgcggcctac
     6481 tcgcgcgtct gcctggcggg gcaagtccga gagcagccgc ggccgccgcg tgtactacga
     6541 tgaaggggtg gccagcagcg acgatgactc cagcggagac gagtccgacg atgggacctc
     6601 cggatgcctc cgctggtttc agcatcggcg aaatcgccgc cgccgaaagc cccagcgcaa
     6661 cttactccgc aactttctcg tgcaagcctt cgggggctgc ttcggtcgat ctgagagtcc
     6721 ccagcccaaa gcctcgcgct ctctcaaggt caagaaggta cccctggcgg agaagcgcag
     6781 acagatgcgc aaagaagccc tggagaagcg ggcccagaag cgcgcagaga agaaacgcag
     6841 taagctcatc gacaaacaac tccaggacga aaagatgggc tacatgtgta cgcaccgcct
     6901 gctgcttcta ggctagctag gcggactgtt actgagctgc gttttacacc ctttctttga
     6961 caaaacctaa cttgcgcaga aaaaaaaaaa ataagagaca acattggcat ggctttgttt
     7021 ttttaaattt tttttaaagt tttttttttt tttttttttt ttttttttaa gtttttttgt
     7081 tttgttttgg cgcttttgac tcaggattta aaaactggaa cggtgaaggc gacagcagtt
     7141 ggttggagca aacatccccc aaagttctac aaatgtggct gaggactttg tacattgttt
     7201 tgtttttttt tttttttggt tttgtctttt tttaatagtc attccaagta tccatgaaat
     7261 aagtggttac aggaagtccc tcaccctccc aaaagccacc cccactccta agaggaggat
     7321 ggtcgcgtcc atgccctgag tccaccccgg ggaaggtgac aaccggtaac ctacaaacgg
     7381 gtggaggatc accccacccg acacttcaca atcaaggggt acaatacaca agggtggagg
     7441 aacaccccac cctccagaca cattacacag aaatccaatc aaacagaagc accatcaggg
     7501 cttctgctac caaatttatc tcaaaaaact acaacaagga atcaccatca gggattccct
     7561 gtgcaatata cgtcaaacga gggccacgac gggaggacga tcacgcctcc cgaatatcgg
     7621 catgtctggc tttcgaattc agtgcgtgga gcatcagccc acgcagccaa tcagagtcga
     7681 atacaagtcg actttcgcga agagcatcag ccttcgcgcc attcttacac aaaccacact
     7741 ctcccctaca ggaacagcat cagcgttcct gcccagtacc caactcaaga aaatttatgt
     7801 ccccatgcag catcagcgca tgggccccaa gaatacatcc ccaacaaaat cacatccgag
     7861 caccaacagg gctcggagtg ttgtttcttg tccaactgga caaaccctcc atggaccatc
     7921 aggccatgga ctctcaccaa caagacaaaa actactcttc tcgaagcagc atcagcgctt
     7981 cgaaacactc gagcatacat tgtgcctatt tcttgggtgg acgatcacgc cacccatgct
     8041 ctcacgaatt tcaaaacacg gacaaggacg agcaccacca gggctcgtcg ttccacgtcc
     8101 aatacgatta cttacctttc gggatcacga tcacggatcc cgcagctaca tcacttccac
     8161 tcaggacatt caagcatgca cgatcacggc atgctccaca agtctcaacc acagaaacta
     8221 ccaaatgggt tcagcaccag cgaacccact cctacctcaa acctcttccc acaaaactgg
     8281 caagcaggat caccgcttgc ccattccaac ataccaaatc aaaaacaatt actggtacag
     8341 catcagcgta ccagcccaca tctctcacta ctatcaaaaa ccaaaccgtt cagcaacagc
     8401 gaacggtaca cacggaaaaa tcaactggtt tacaaatacg aaagacgatc acgctttcgt
     8461 ccagcgcaaa ctattacgaa aaacatccga cgggaagagc aacagccttc ccgcggcgga
     8521 aaacctcaca aaaacacgac aaacggatgc acgaacacgg catccgccga caacccacaa
     8581 acttacaacc aggcaaacgg tgcaggatca ccgcaccgta catcaaacac ctcagatctc
     8641 atatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     9001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     9061 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9181 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     9241 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9301 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9361 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9421 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9481 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9541 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9601 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
     9661 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     9721 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9841 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10081 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
    10141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    11641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    11701 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
    11761 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    11821 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
    11881 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    11941 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    12001 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    12061 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    12121 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    12181 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    12241 taaatatgta ctacaaactt agtagt
//
