LOCUS       apobb.1_homology_arm_5'_gateway_clone_        3183 bp    DNA     circular UNA 13-JUN-2019
DEFINITION  an entry clone created from pDONR™ P4-P1r and recombination-seq:.
ACCESSION   urn.local...4k-a4uy03x
VERSION     urn.local...4k-a4uy03x
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     terminator      268..295
                     /note="<html><body>transcription terminator T2 from the
                     <i>E. coli rrnB</i> gene</body></html>"
                     /standard_name="rrnB T2 terminator"
     terminator      387..473
                     /gene="<html><body><i>Escherichia coli
                     rrnB</i></body></html>"
                     /note="<html><body>transcription terminator T1 from the
                     <i>E. coli rrnB</i> gene</body></html>"
                     /standard_name="rrnB T1 terminator"
     primer_bind     537..553
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 fwd"
     misc_feature    592..688
                     /note="Geneious type: attL4"
                     /standard_name="attL4"
     protein_bind    593..>674
                     /gene="<html><body>mutant version of
                     <i>att</i>P</body></html>"
                     /note="<html><body>recombination site for the Gateway® BP
                     reaction</body></html>"
                     /bound_moiety="<html><body>BP Clonase™</body></html>"
                     /standard_name="attP4"
     misc_feature    complement(689..1228)
                     /standard_name="Left homology arm"
     misc_feature    1229..1352
                     /note="Geneious type: attR1"
                     /standard_name="attR1"
     protein_bind    <1236..1385
                     /gene="<html><body>mutant version of
                     <i>att</i>P</body></html>"
                     /note="<html><body>recombination site for the Gateway® BP
                     reaction</body></html>"
                     /bound_moiety="<html><body>BP Clonase™</body></html>"
                     /standard_name="attP1"
     primer_bind     complement(1448..1464)
                     /note="<html><body>common sequencing primer, one of
                     multiple similar variants</body></html>"
                     /standard_name="M13 rev"
     CDS             1577..2386
                     /product="<html><body>aminoglycoside
                     phosphotransferase</body></html>"
                     /transl_table=1
                     /gene="<html><body><i>aph(3')-Ia</i></body></html>"
                     /note="<html><body>confers resistance to kanamycin in
                     bacteria or G418 (Geneticin®) in eukaryotes</body></html>"
                     /codon_start=1
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /standard_name="KanR"
     rep_origin      2533..3121
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgcg ctgcctggaa tgaatgaagc tacactggct
      721 gaaatttgca agaaaatcca attagttgtt gtagaaatgc tgaggaattt tggtaacaac
      781 ttagaggtat acttctctcc catcatggat aactttaata caattcaatt gacatttccc
      841 aatggaaagg tcatgacagt agctgagttc caacaaaatg tgcaaagtat cctgagaagc
      901 aacctagtta tgatagcaga tgcaatgaag caaatcgaaa gtcctgatgt agttcttaag
      961 aaacttggac aaactttaca agaggttgtt gaaaaaggac aagaatttgt ggacaaaatg
     1021 aatttaagtt tattagaaga tattgccgcg gccattaaca cgttctacca ggagctgatg
     1081 aagatcatag aagacattag tgaggcagtc atctttggtt tttcaatccc tgcaattaaa
     1141 atatatgaaa tttctcaaaa catagggaaa gtgttgaatg caaataatgg aatacatcaa
     1201 tttgagcttc ctctcccatt tttccagaca agtttgtaca aaaaagttga acgagaaacg
     1261 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     1321 tactgtaaaa cacaacatat gcagtcacta tgaatcaact acttagatgg tattagtgac
     1381 ctgtagaatt cgagctctag agctgcaggg cggccgcgat atcccctata gtgagtcgta
     1441 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     1501 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1561 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1621 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1681 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1741 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1801 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1861 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1921 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1981 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2041 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2101 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     2161 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2221 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     2281 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     2341 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     2401 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     2461 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     2521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2641 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     2941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3001 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3061 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3121 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3181 gtt
//
