LOCUS       apobb.1_homology_arm_3'_gateway_clone_        3398 bp    DNA     circular UNA 13-JUN-2019
DEFINITION  an entry clone created from pDONR P2R-P3 and recombination-seq:
            Copy.
ACCESSION   urn.local...1j-al3wziv
VERSION     urn.local...1j-al3wziv
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>733,<1514..3398)
                     /organism="pDONR P2R-P3"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /standard_name="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /standard_name="rrnB_T1_terminator"
     misc_feature    522..544
                     /standard_name="M13_pUC_fwd_primer"
     promoter        537..553
                     /standard_name="M13_forward20_primer"
     misc_feature    complement(591..>733)
                     /standard_name="attP"
     misc_feature    complement(624..747)
                     /note="Geneious type: attR2"
                     /standard_name="attR2"
     misc_feature    636..724
                     /standard_name="attR2"
     misc_feature    749..1507
                     /standard_name="right homology arm"
     misc_feature    1221..1223
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    complement(1507..1603)
                     /note="Geneious type: attL3"
                     /standard_name="attL3"
     misc_feature    complement(<1514..1602)
                     /standard_name="attP"
     promoter        complement(1640..1658)
                     /standard_name="T7_promoter"
     promoter        complement(1659..1677)
                     /standard_name="M13_reverse_primer"
     CDS             1792..2601
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /standard_name="ORF frame 2"
     gene            1792..2601
                     /gene="KanR2"
                     /standard_name="KanR2"
     rep_origin      2732..3351
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggaat atggtcattc tgaacagaaa gtaaattaag
      781 taaacactac catcttttga aatgaacatt cttggacatc gaagccaatg gacacttcac
      841 tgaagtatag tacaaacatg tatataaagc aattactgac tcaaaacatg tctcattgtt
      901 atgctgctct attattctat ttgtataaat gaggattgaa atactaagct agttatcaaa
      961 aatgtcaact gcaacctatt gtcatccttg ttctgtatcc aacattaatg gcatgatgga
     1021 tggggatcaa taaagaactt ttattatgca acacgaccca atcttgtcat ttctatattc
     1081 tagaagtgtt ttaaactagt ttggaccatt caataatata atataattga atatacaggt
     1141 gtaataacat gcaacaaaat gaaaaataag cagtggtaca tgggtacatt ttagtttttt
     1201 atgcaaacta ctggtgactt taaaaaaaaa attacaaata aaatatttta ttctattttt
     1261 ttcattagga agaaaaaagt ttatcagaac ttttgtagag gtaaaaaaaa ctactaacat
     1321 tttactcaat cccatacctg aaaaatctag taaatcaatt gctttgatta gtggttattg
     1381 aaccttattt taaccagctt caggttaaca catgttaact actccttttt gacatataaa
     1441 ctgaaaatca tttaatcttc attaaaaata ttttggattt gcttacaaac tgatgtctgc
     1501 cttacccaac tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt
     1561 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt
     1621 taacgcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1681 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1741 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1801 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1861 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1921 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1981 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2041 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2101 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2161 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2221 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2281 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2341 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2401 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2461 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2521 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2581 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2641 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2881 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3121 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3361 cggttcctgg ccttttgctg gccttttgct cacatgtt
//
