LOCUS       pT3TS-TAL-PR1-RA        5941 bp    DNA     circular UNA 13-JUN-2019
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        146..165
                     /note="T3 Promoter"
                     /standard_name="T3 Promoter"
     5'UTR           173..216
                     /note="Xenopus beta-globin 5' UTR"
                     /standard_name="5'UTR"
     misc_feature    270..272
                     /note="start codon"
                     /standard_name="start codon"
     misc_feature    710..746
                     /note="left"
                     /standard_name="left"
     misc_feature    complement(741..746)
                     /standard_name="BsMBI"
     source          <749..>952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    749..2476
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCA
                     ACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
                     GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGT
                     TGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTA
                     CCGGGCCCCCCCTCGAGGTCCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGC
                     GCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT
                     TCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTG
                     AGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCACCGG"
     misc_feature    845..850
                     /standard_name="NG"
     misc_feature    947..952
                     /standard_name="NG"
     misc_feature    <1050..1054
                     /standard_name="HD"
     misc_feature    1151..1156
                     /standard_name="NG"
     misc_feature    1253..1258
                     /standard_name="NN"
     misc_feature    1355..1360
                     /standard_name="NG"
     misc_feature    1457..1462
                     /standard_name="NG"
     misc_feature    1559..1564
                     /standard_name="HD"
     misc_feature    1661..1666
                     /standard_name="NI"
     misc_feature    1763..1768
                     /standard_name="NN"
     misc_feature    <1866..1870
                     /standard_name="NI"
     misc_feature    complement(<1968..1972)
                     /standard_name="NI"
     misc_feature    2069..2074
                     /standard_name="NG"
     misc_feature    <2172..2176
                     /standard_name="NN"
     misc_feature    2273..2278
                     /standard_name="NI"
     misc_feature    2375..2380
                     /standard_name="HD"
     misc_feature    2478..2518
                     /note="right"
                     /standard_name="right"
     misc_feature    2478..2483
                     /standard_name="BsMBI"
     misc_feature    2635..3222
                     /note="FokI Homodimer CDS"
                     /standard_name="FokI Homodimer CDS"
     modified_base   3083..3085
                     /note="D450A codon"
     3'UTR           3247..3417
                     /note="Xenopus beta-globin 3' UTR"
                     /standard_name="3' UTR"
     misc_feature    3448..3454
                     /note="Missing sites"
                     /standard_name="Missing sites"
     misc_feature    3479..3481
                     /note="missing bases"
                     /standard_name="missing bases"
     promoter        complement(3496..3516)
                     /note="T7"
                     /standard_name="T7"
     rep_origin      3679..4135
     CDS             4266..5123
                     /codon_start=1
                     /note="Amp"
                     /note="Amp resistance gene"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="Amp"
     rep_origin      5187..>5941
ORIGIN      
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgaatta accctcacta aagggaagct tgcttgttct
      181 ttttgcagaa gctcagaata aacgctcaac tttggcagat ctaactcgag aaagatattg
      241 tatatatcgt aacaatagga ggttcaacaa tggcttcctc ccctccaaag aaaaagagaa
      301 aggttagttg gaaggacgca agtggttggt ctagagtgga tctacgcacg ctcggctaca
      361 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg
      421 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg
      481 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga
      541 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct
      601 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg
      661 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg
      721 cactgacggg tgcccccctg gagacggggg cggcaagcaa gcgctcgaaa cggtgcagcg
      781 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
      841 cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
      901 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa
      961 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     1021 cccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt
     1081 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
     1141 tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     1201 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg
     1261 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     1321 cctgaccccg gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc aagcgctcga
     1381 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
     1441 ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     1501 gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca
     1561 cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     1621 ccatggcctg accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc
     1681 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tcacacccga
     1741 tcaggtcgtg gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg
     1801 gctgnngccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg tggctatcgc
     1861 cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     1921 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca ttggcggcaa
     1981 gcaagcgctc gaaacggtgc agcsgctgtt gccggtgctg tgccaggacc atggcctgac
     2041 cccggaccaa gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt
     2101 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg actccggacc aagtggtggc
     2161 tatcgccagc aacaatggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     2221 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg
     2281 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     2341 cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc aagcgctcga
     2401 aagcattgtg gcccagctga gccggcctga tccggcgttg gccgcgttga ccaacgacca
     2461 cctcgtcgcc nnggcctcgt ctccaacgac cacctcgtcg ccttggcctg cctcggcgga
     2521 cgtcctgcca tggatgcagt gaaaaaggga ttgccgcacg cgccggaatt gatcagaaga
     2581 gtcaatcgcc gtattggcga acgcacgtcc catcgcgttg cctctagatc ccagctagtg
     2641 aaatctgaat tggaagagaa gaaatctgaa cttagacata aattgaaata tgtgccacat
     2701 gaatatattg aattgattga aatcgcaaga aattcaactc aggatagaat ccttgaaatg
     2761 aaggtgatgg agttctttat gaaggtttat ggttatcgtg gtaaacattt gggtggatca
     2821 aggaaaccag acggagcaat ttatactgtc ggatctccta ttgattacgg tgtgatcgtt
     2881 gatactaagg catattcagg aggttataat cttccaattg gtcaagcaga tgaaatgcaa
     2941 agatatgtcg aagagaatca aacaagaaac aagcatatca accctaatga atggtggaaa
     3001 gtctatccat cttcagtaac agaatttaag ttcttgtttg tgagtggtca tttcaaagga
     3061 aactacaaag ctcagcttac aagattgaat catatcacta attgtaatgg agctgttctt
     3121 agtgtagaag agcttttgat tggtggagaa atgattaaag ctggtacatt gacacttgag
     3181 gaagtgagaa ggaaatttaa taacggtgag ataaactttt aataggctag tgactgacta
     3241 ggatctggtt accactaaac cagcctcaag aacacccgaa tggagtctct aagctacata
     3301 ataccaactt acacttacaa aatgttgtcc cccaaaatgt agccattcgt atctgctcct
     3361 aataaaaaga aagtttcttc acattctaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaccc
     3421 cccccccccc cccccccccc cccccccgca tgcctgcagg tcgactagga tccccgggta
     3481 ccgagctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     3541 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3601 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     3661 agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     3721 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3781 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3841 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3901 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3961 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4021 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4081 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     4141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4261 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4321 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4381 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4441 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4501 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4561 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4621 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4681 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4741 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4801 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4861 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4921 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4981 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5041 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5101 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5161 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5941 a
//
